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Q5R6L3 (DNLI4_PONAB) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA ligase 4

EC=6.5.1.1
Alternative name(s):
DNA ligase IV
Polydeoxyribonucleotide synthase [ATP] 4
Gene names
Name:DNL4
OrganismPongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii) [Reference proteome]
Taxonomic identifier9601 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaePongo

Protein attributes

Sequence length911 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends By similarity.

Catalytic activity

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).

Cofactor

Magnesium By similarity.

Subunit structure

Binds to XRCC4. The LIG4-XRCC4 complex has probably a 1:2 stoichiometry. The LIG4-XRCC4 heteromer associates in a DNA-dependent manner with the DNA-dependent protein kinase complex DNA-PK, formed by the Ku p70/p86 dimer (G22P1/G22P2) and PRKDC. Interacts with APLF By similarity.

Subcellular location

Nucleus By similarity.

Sequence similarities

Belongs to the ATP-dependent DNA ligase family.

Contains 2 BRCT domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 911911DNA ligase 4
PRO_0000059578

Regions

Domain654 – 74390BRCT 1
Domain808 – 911104BRCT 2

Sites

Active site2731N6-AMP-lysine intermediate By similarity
Metal binding3311Magnesium 1 Potential
Metal binding4271Magnesium 2 Potential
Binding site2711ATP By similarity
Binding site2781ATP By similarity
Binding site2931ATP By similarity
Binding site4321ATP By similarity
Binding site4431ATP By similarity
Binding site4491ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5R6L3 [UniParc].

Last modified December 21, 2004. Version 1.
Checksum: C6AD9817E9EC2B59

FASTA911104,053
        10         20         30         40         50         60 
MAASQTSQTV ASHVPFADLC STLERIQKSK GRAEKIRHFR EFLDSWRKFH DAFHKNQKDV 

        70         80         90        100        110        120 
TDSFYPAMRL ILPQLERERM AYGIKETMLA KLYIELLNLP RDGKDALKLL NYRTPTGTHG 

       130        140        150        160        170        180 
DAGDFAMIAY FVLKPRCLQK GSLTIQQVND LLDSIASNNS AKRKDLIKKS LLQLITQSSA 

       190        200        210        220        230        240 
LEQKWLIRMI IKDLKLGVSQ QTIFSVFHSD AVELHNVTTD LEKVCRQLHD PSVGLSDISI 

       250        260        270        280        290        300 
TLFSAFKPML AAIADIEHIE KDMKHQSFYI ETKLDGERMQ MHKDGDVYKY FSRNGYNYTD 

       310        320        330        340        350        360 
QFGASPTEGS LTPFIHNAFK TDIQICILDG EMMAYNPNTQ TFMQKGTKFD IKRMVEDSDL 

       370        380        390        400        410        420 
QTCYCVFDVL MVNNKKLGHE TLRKRYEILS SIFTPIPGRI EIVQKTQAHT KNEVIDALNE 

       430        440        450        460        470        480 
AIDKREEGIM IKQPLSIYKP DKRGEGWLKI KPEYVSGLMD ELDILIVGGY WGKGSRGGMM 

       490        500        510        520        530        540 
SHFLCAVAEK PPPGEKPSVF HTLSRVGSGC TMKELYDLGL KLAKYWKPFH KKAPPSSILC 

       550        560        570        580        590        600 
GTEKPEVYIE PCNSVIVQIK AAEIVPSDMY KTGCTLRFPR IEKIRDDKEW HECMTLDDLE 

       610        620        630        640        650        660 
QLRGKASGKL ASKHFYVGGD DEPQEKKRKA APKMKKVIGI IEHLKAPNLT NVNKISNIFE 

       670        680        690        700        710        720 
DVEFCVMSGT DSQPKPDLEN RIAEFGGYIV QNPGPDTYCV IAGSKNIRVK NIILSNKHDV 

       730        740        750        760        770        780 
VKPAWLLECF KTKSFVPWQP HFMIHMCPST KEHFAREYDC YGDSYFVDTD LNQLKEVFSG 

       790        800        810        820        830        840 
IKNSNEQTPE EMASLIADLE YRYSWDCSPL SMFRRHTVYL DLYAVINDLS TKNEGTRLAI 

       850        860        870        880        890        900 
KALELRFHGA KVVSCLAEGV SHVIIGEDHS RIADFKAFRR TFKRKFKILK ESWITDSIDK 

       910 
CELQEENQYL I 

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References

[1]The German cDNA consortium
Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain cortex.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CR860475 mRNA. Translation: CAH92597.1.
RefSeqNP_001126522.1. NM_001133050.1.

3D structure databases

ProteinModelPortalQ5R6L3.
SMRQ5R6L3. Positions 654-759.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ5R6L3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID100173511.
KEGGpon:100173511.

Organism-specific databases

CTD3981.

Phylogenomic databases

HOVERGENHBG005516.
InParanoidQ5R6L3.
KOK10777.

Family and domain databases

Gene3D1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
3.40.50.10190. 2 hits.
InterProIPR001357. BRCT_dom.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR021536. DNA_ligase_IV.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamPF00533. BRCT. 2 hits.
PF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
PF11411. DNA_ligase_IV. 1 hit.
[Graphical view]
SMARTSM00292. BRCT. 2 hits.
[Graphical view]
SUPFAMSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF52113. SSF52113. 2 hits.
TIGRFAMsTIGR00574. dnl1. 1 hit.
PROSITEPS50172. BRCT. 2 hits.
PS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDNLI4_PONAB
AccessionPrimary (citable) accession number: Q5R6L3
Entry history
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: December 21, 2004
Last modified: October 16, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families