Q5R646 (ECHM_PONAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Enoyl-CoA hydratase, mitochondrial
EC=4.2.1.17

Alternative name(s):
Enoyl-CoA hydratase 1
Short-chain enoyl-CoA hydratase
Short name=SCEH

Gene names

Name:

ECHS1

Organism

Pongo abelii (Sumatran orangutan)

Taxonomic identifier

9601 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo

Protein attributes

Sequence length	290 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Straight-chain enoyl-CoA thioesters from C4 up to at least C16 are processed, although with decreasing catalytic rate By similarity.
Catalytic activity	(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.
Pathway	Lipid metabolism; fatty acid beta-oxidation.
Subunit structure	Homohexamer; dimer of trimers By similarity.
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the enoyl-CoA hydratase/isomerase family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Cellular component	Mitochondrion
Domain	Transit peptide
Molecular function	Lyase
PTM	Acetylation
Gene Ontology (GO)
Biological process	fatty acid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	enoyl-CoA hydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 27	27	Mitochondrion By similarity
	Chain	28 – 290	263	Enoyl-CoA hydratase, mitochondrial		PRO_0000007413
Regions
	Region	98 – 101	4	Substrate binding By similarity
Sites
	Binding site	141	1	Substrate; via amide nitrogen By similarity
	Site	164	1	Important for catalytic activity By similarity
Amino acid modifications
	Modified residue	101	1	N6-acetyllysine By similarity
	Modified residue	118	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5R646 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: 53636860E6592104	FASTA	290	31,428
	10 20 30 40 50 60 MATLRVLLSC VRGPLRPPVR CPAWRPFASG ANFEYIIAEK RGKNNTVGLI QLNRPKALNA 70 80 90 100 110 120 LCDGLIDELN QALKIFEEDP AVGAIVLTGG DKAFAAGADI KEMQNLSFQD CYSSKFLKHW 130 140 150 160 170 180 DHLTQIKKPV IAAVNGYAFG GGCELAMMCD IIYAGEKAQF AQPEILIGTI PGAGGTQRLT 190 200 210 220 230 240 RTVGKSLAME MVLTGDRISA QDAKQAGLVS KICPVETLVE EAIQCAEKIA SNSKIIAAMA 250 260 270 280 290 KESVNAAFEM TLAEGSKLEK KLFYSTFATN DRKEGMTAFV EKRKANFKDQ

References

[1]	The German cDNA consortium Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain cortex.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR860650 mRNA. Translation: CAH92770.1.
RefSeq	NP_001126615.1. NM_001133143.1.
UniGene	Pab.18315.
3D structure databases
ProteinModelPortal	Q5R646.
SMR	Q5R646. Positions 31-290.
ModBase	Search...
Proteomic databases
PRIDE	Q5R646.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100173612.
KEGG	pon:100173612.
Organism-specific databases
CTD	1892.
Phylogenomic databases
HOVERGEN	HBG010157.
InParanoid	Q5R646.
Family and domain databases
InterPro	IPR014748. Crontonase_C. IPR001753. Crotonase_core. IPR018376. Enoyl-CoA_hyd/isom_CS. [Graphical view]
Gene3D	G3DSA:1.10.12.10. Crontonase_C. 1 hit.
KO	K07511.
Pfam	PF00378. ECH. 1 hit. [Graphical view]
PROSITE	PS00166. ENOYL_COA_HYDRATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ECHM_PONAB

Accession

Primary (citable) accession number: Q5R646

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	December 21, 2004
Last modified:	November 16, 2011
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program