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Q5R636

- PFKAP_PONAB

UniProt

Q5R636 - PFKAP_PONAB

Protein

ATP-dependent 6-phosphofructokinase, platelet type

Gene

PFKP

Organism
Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 63 (01 Oct 2014)
      Sequence version 1 (21 Dec 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

    Catalytic activityi

    ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Enzyme regulationi

    Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei34 – 341ATP; via amide nitrogenUniRule annotation
    Metal bindingi128 – 1281Magnesium; catalyticUniRule annotation
    Active sitei175 – 1751Proton acceptorUniRule annotation
    Binding sitei210 – 2101Substrate; shared with dimeric partnerUniRule annotation
    Binding sitei273 – 2731SubstrateUniRule annotation
    Binding sitei301 – 3011Substrate; shared with dimeric partnerUniRule annotation
    Binding sitei481 – 4811Allosteric activator fructose 2,6-bisphosphateUniRule annotation
    Binding sitei576 – 5761Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation
    Binding sitei639 – 6391Allosteric activator fructose 2,6-bisphosphateUniRule annotation
    Binding sitei665 – 6651Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation
    Binding sitei744 – 7441Allosteric activator fructose 2,6-bisphosphateUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi97 – 982ATPUniRule annotation
    Nucleotide bindingi127 – 1304ATPUniRule annotation

    GO - Molecular functioni

    1. 6-phosphofructokinase activity Source: UniProtKB
    2. ATP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. carbohydrate phosphorylation Source: GOC
    2. fructose 6-phosphate metabolic process Source: InterPro
    3. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent 6-phosphofructokinase, platelet typeUniRule annotation (EC:2.7.1.11UniRule annotation)
    Short name:
    ATP-PFKUniRule annotation
    Short name:
    PFK-P
    Alternative name(s):
    6-phosphofructokinase type C
    Phosphofructo-1-kinase isozyme C
    Short name:
    PFK-C
    PhosphohexokinaseUniRule annotation
    Gene namesi
    Name:PFKP
    OrganismiPongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
    Taxonomic identifieri9601 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaePongo
    ProteomesiUP000001595: Unplaced

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. 6-phosphofructokinase complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 784784ATP-dependent 6-phosphofructokinase, platelet typePRO_0000284442Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei12 – 121PhosphoserineBy similarity
    Modified residuei386 – 3861PhosphoserineBy similarity
    Modified residuei395 – 3951N6-acetyllysineBy similarity
    Modified residuei486 – 4861N6-acetyllysineBy similarity
    Glycosylationi540 – 5401O-linked (GlcNAc)By similarity
    Modified residuei651 – 6511PhosphotyrosineBy similarity
    Modified residuei688 – 6881N6-acetyllysineBy similarity
    Modified residuei783 – 7831PhosphoserineBy similarity

    Post-translational modificationi

    GlcNAcylation decreases enzyme activity.By similarity

    Keywords - PTMi

    Acetylation, Glycoprotein, Phosphoprotein

    Proteomic databases

    PRIDEiQ5R636.

    Interactioni

    Subunit structurei

    Homo- and heterotetramers.UniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliQ5R636.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 399399N-terminal catalytic PFK domain 1Add
    BLAST
    Regioni173 – 1753Substrate bindingUniRule annotation
    Regioni217 – 2193Substrate bindingUniRule annotation
    Regioni307 – 3104Substrate bindingUniRule annotation
    Regioni400 – 41112Interdomain linkerAdd
    BLAST
    Regioni412 – 784373C-terminal regulatory PFK domain 2Add
    BLAST
    Regioni538 – 5425Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation
    Regioni583 – 5853Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation
    Regioni671 – 6744Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.UniRule annotation

    Phylogenomic databases

    HOVERGENiHBG000976.
    InParanoidiQ5R636.
    KOiK00850.

    Family and domain databases

    HAMAPiMF_03184. Phosphofructokinase_I_E.
    InterProiIPR009161. 6-phosphofructokinase_euk.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view]
    PfamiPF00365. PFK. 2 hits.
    [Graphical view]
    PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 2 hits.
    TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
    PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5R636-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDADDSRAPK GSLRKFLEHL SGAGKAIGVL TSGGDAQGMN AAVRAVVRMG    50
    IYVGAKVCFI YEGYQGMVDG GSNIAEADWE SVSSILQVGG TIIGSARCQA 100
    FRTREGRLKA ACNLLQRGIT NLCVIGGDGS LTGANLFRKE WSGLLEELAR 150
    NGQIDKEAVQ KYAYLNVVGM VGSIDNDFCG TDMTIGTDSA LHRIIEVVDA 200
    IMTTAQSHQR TFVLEVMGRH CGYLALVSAL ACGADWVFLP ESPPEEGWEE 250
    QMCVKLSENR ARKKRLNIII VAEGAIDTQN KPITSEKIKE LVVTQLGYDT 300
    RVTILGHVQR GGTPSAFDRI LASRMGVEAV IALLEATPDT PACVVSLNGN 350
    HAVRLPLMEC VQMTQDVQKA MDERRFQDAV RLRGRSFAGN LNTYKRLAIK 400
    LPDDQIPKTN CNVAVINVGA PAAGMNAAVR SAVRVGIADG HRMLAIYDGF 450
    DGFAKGQIKE IGWTDVGGWT GQGGSILGTK RVLPGKYLEE IATQMRTHSI 500
    NALLIIGGFE AYLGLLELSA AREKHEEFCV PMVMVPATVS NNVPGSDFSI 550
    GADTALNTIT DTCDRIKQSA SGTKRRVFII ETMGGYCGYL ANMGGLAAGA 600
    DAAYIFEEPF DIRDLQSSVE HLTEKMKTTI QRGLVLRNES CSENYTTDFI 650
    YQLYSEEGKG VFDCRKNVLG HMQQGGAPSP FDRNFGTKIS ARAMEWITAK 700
    LKETRGRGKK FTTDDSVCVL GISKRNVIFQ PVAELKKQTD FEHRIPKEQW 750
    WLKLRPLMKI LAKYKASYDV SDSGQLEHVQ PWSV 784
    Length:784
    Mass (Da):85,525
    Last modified:December 21, 2004 - v1
    Checksum:i6DC468B4629E235D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR860660 mRNA. Translation: CAH92780.1.
    RefSeqiNP_001126622.1. NM_001133150.1.
    UniGeneiPab.4970.

    Genome annotation databases

    GeneIDi100173619.
    KEGGipon:100173619.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR860660 mRNA. Translation: CAH92780.1 .
    RefSeqi NP_001126622.1. NM_001133150.1.
    UniGenei Pab.4970.

    3D structure databases

    ProteinModelPortali Q5R636.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q5R636.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100173619.
    KEGGi pon:100173619.

    Organism-specific databases

    CTDi 5214.

    Phylogenomic databases

    HOVERGENi HBG000976.
    InParanoidi Q5R636.
    KOi K00850.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00182 .

    Family and domain databases

    HAMAPi MF_03184. Phosphofructokinase_I_E.
    InterProi IPR009161. 6-phosphofructokinase_euk.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view ]
    Pfami PF00365. PFK. 2 hits.
    [Graphical view ]
    PIRSFi PIRSF000533. ATP_PFK_euk. 1 hit.
    PRINTSi PR00476. PHFRCTKINASE.
    SUPFAMi SSF53784. SSF53784. 2 hits.
    TIGRFAMsi TIGR02478. 6PF1K_euk. 1 hit.
    PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. The German cDNA consortium
      Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain cortex.

    Entry informationi

    Entry nameiPFKAP_PONAB
    AccessioniPrimary (citable) accession number: Q5R636
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 17, 2007
    Last sequence update: December 21, 2004
    Last modified: October 1, 2014
    This is version 63 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3