Q5R5Z6 (UBP20_PONAB) Reviewed, UniProtKB/Swiss-Prot
Last modified
April 3, 2013.
Version 51.
History...
Clusters with 
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Ubiquitin carboxyl-terminal hydrolase 20 EC=3.4.19.12 Alternative name(s): Deubiquitinating enzyme 20 Ubiquitin thioesterase 20 Ubiquitin-specific-processing protease 20 | ||
| Gene names |
| ||
| Organism | Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii) [Reference proteome] | ||
| Taxonomic identifier | 9601 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo |
Protein attributes
| Sequence length | 913 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at transcript level |
General annotation (Comments)
| Function | Deubiquitinating enzyme involved in beta-2 adrenergic receptor (ADRB2) recycling. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination beta-2 adrenergic receptor (ADRB2). Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta-arrestins, possibly leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Deubiquitinates HIF1A, leading to stabilize HIF1A and enhance HIF1A-mediated activity. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains By similarity. |
| Catalytic activity | Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). |
| Subunit structure | Interacts with VHL, leading to its ubiquitination and subsequent degradation. Interacts with DIO2 and HIF1A. |
| Subcellular location | Cytoplasm › perinuclear region By similarity. |
| Domain | The UBP-type zinc finger binds 3 zinc ions. However, it does not bind ubiquitin, probably because the conserved Arg in position 55 is replaced by a Glu residue By similarity. |
| Post-translational modification | Ubiquitinated via a VHL-dependent pathway for proteasomal degradation By similarity. |
| Sequence similarities | Belongs to the peptidase C19 family. USP20/USP33 subfamily. Contains 2 DUSP domains. Contains 1 UBP-type zinc finger. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 913 | 913 | Ubiquitin carboxyl-terminal hydrolase 20 | PRO_0000390419 | |||||
Regions | |||||||||
| Domain | 686 – 779 | 94 | DUSP 1 | ||||||
| Domain | 788 – 891 | 104 | DUSP 2 | ||||||
| Zinc finger | 28 – 92 | 65 | UBP-type | ||||||
Sites | |||||||||
| Active site | 153 | 1 | Nucleophile By similarity | ||||||
| Active site | 642 | 1 | Proton acceptor By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 131 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 133 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 257 | 1 | Phosphothreonine By similarity | ||||||
| Modified residue | 376 | 1 | Phosphothreonine By similarity | ||||||
| Modified residue | 412 | 1 | Phosphoserine By similarity | ||||||
Sequences
| ||||||||||||||||||
References
| [1] | The German cDNA consortium Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain cortex. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | CR860704 mRNA. Translation: CAH92820.1. |
| RefSeq | NP_001126647.1. NM_001133175.1. |
| UniGene | Pab.18678. |
3D structure databases | |
| ProteinModelPortal | Q5R5Z6. |
| SMR | Q5R5Z6. Positions 5-97. |
| ModBase | Search... |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| GeneID | 100173645. |
| KEGG | pon:100173645. |
Organism-specific databases | |
| CTD | 10868. |
Phylogenomic databases | |
| HOGENOM | HOG000286031. |
| HOVERGEN | HBG054196. |
| InParanoid | Q5R5Z6. |
| KO | K11848. |
Family and domain databases | |
| Gene3D | 3.30.40.10. 1 hit. |
| InterPro | IPR006615. Pept_C19_DUSP. IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. IPR013083. Znf_RING/FYVE/PHD. IPR001607. Znf_UBP. [Graphical view] |
| Pfam | PF06337. DUSP. 1 hit. PF00443. UCH. 1 hit. PF02148. zf-UBP. 1 hit. [Graphical view] |
| SMART | SM00695. DUSP. 2 hits. [Graphical view] |
| PROSITE | PS51283. DUSP. 2 hits. PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. PS50271. ZF_UBP. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | UBP20_PONAB | ||||||||
| Accession | Primary (citable) accession number: Q5R5Z6 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
Relevant documents
| Peptidase families Classification of peptidase families and list of entries |
| SIMILARITY comments Index of protein domains and families |