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Q5R5Z6

- UBP20_PONAB

UniProt

Q5R5Z6 - UBP20_PONAB

Protein

Ubiquitin carboxyl-terminal hydrolase 20

Gene

USP20

Organism
Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 59 (01 Oct 2014)
      Sequence version 1 (21 Dec 2004)
      Previous versions | rss
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    Functioni

    Deubiquitinating enzyme involved in beta-2 adrenergic receptor (ADRB2) recycling. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination beta-2 adrenergic receptor (ADRB2). Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta-arrestins, possibly leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Deubiquitinates HIF1A, leading to stabilize HIF1A and enhance HIF1A-mediated activity. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei153 – 1531NucleophilePROSITE-ProRule annotation
    Active sitei642 – 6421Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri28 – 9265UBP-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. cysteine-type endopeptidase activity Source: UniProtKB
    2. ubiquitin-specific protease activity Source: InterPro
    3. ubiquitin thiolesterase activity Source: UniProtKB
    4. zinc ion binding Source: InterPro

    GO - Biological processi

    1. endocytosis Source: UniProtKB-KW
    2. protein deubiquitination Source: UniProtKB
    3. protein K48-linked deubiquitination Source: UniProtKB
    4. protein K63-linked deubiquitination Source: UniProtKB
    5. regulation of G-protein coupled receptor protein signaling pathway Source: UniProtKB
    6. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Endocytosis, Ubl conjugation pathway

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 20 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 20
    Ubiquitin thioesterase 20
    Ubiquitin-specific-processing protease 20
    Gene namesi
    Name:USP20
    OrganismiPongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
    Taxonomic identifieri9601 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaePongo
    ProteomesiUP000001595: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. centrosome Source: UniProtKB
    2. perinuclear region of cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 913913Ubiquitin carboxyl-terminal hydrolase 20PRO_0000390419Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei131 – 1311PhosphoserineBy similarity
    Modified residuei133 – 1331PhosphoserineBy similarity
    Modified residuei257 – 2571PhosphothreonineBy similarity
    Modified residuei367 – 3671PhosphoserineBy similarity
    Modified residuei376 – 3761PhosphothreonineBy similarity
    Modified residuei412 – 4121PhosphoserineBy similarity

    Post-translational modificationi

    Ubiquitinated via a VHL-dependent pathway for proteasomal degradation.By similarity

    Keywords - PTMi

    Phosphoprotein, Ubl conjugation

    Interactioni

    Subunit structurei

    Interacts with VHL, leading to its ubiquitination and subsequent degradation. Interacts with CCP110, DIO2 and HIF1A By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ5R5Z6.
    SMRiQ5R5Z6. Positions 5-97.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini144 – 684541USPAdd
    BLAST
    Domaini686 – 77994DUSP 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini788 – 891104DUSP 2PROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The UBP-type zinc finger binds 3 zinc ions. However, it does not bind ubiquitin, probably because the conserved Arg in position 55 is replaced by a Glu residue By similarity.By similarity

    Sequence similaritiesi

    Contains 2 DUSP domains.PROSITE-ProRule annotation
    Contains 1 UBP-type zinc finger.PROSITE-ProRule annotation
    Contains 1 USP domain.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri28 – 9265UBP-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    HOGENOMiHOG000286031.
    HOVERGENiHBG054196.
    InParanoidiQ5R5Z6.
    KOiK11848.

    Family and domain databases

    Gene3Di3.30.2230.10. 2 hits.
    3.30.40.10. 1 hit.
    InterProiIPR006615. Pept_C19_DUSP.
    IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR001607. Znf_UBP.
    [Graphical view]
    PfamiPF06337. DUSP. 1 hit.
    PF00443. UCH. 1 hit.
    PF02148. zf-UBP. 1 hit.
    [Graphical view]
    SMARTiSM00695. DUSP. 2 hits.
    [Graphical view]
    SUPFAMiSSF143791. SSF143791. 2 hits.
    PROSITEiPS51283. DUSP. 2 hits.
    PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    PS50271. ZF_UBP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5R5Z6-1 [UniParc]FASTAAdd to Basket

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    MGDSRDLCPH LDSIGEVTKE DLLLKSMGTC QSCGVTGPNL WACLQVACPY    50
    VGCGESFADH STIHAQAKKH NLTVNLTTFR LWCYACEKEV FLEQRLAAPL 100
    LGSSKFSEQD SPPPSHPLKA VPIAVADEGE SESEDDDLKP RGLTGMKNLG 150
    NSCYMNAALQ ALSNCPPLTQ FFLECGGLVR TDKKPALCKS YQKLVSEVWH 200
    KKRPSYVVPT SLSHGIKLVN PMFRGYAQQD TQEFLRCLMD QLHEELKEPV 250
    VATVALTEAR DSDSSDTDEK REGDRSPSED EFLSCDSSSD RGEGDGQGRG 300
    GGSSQAETEL LIPDEASRAI SEKERMKDRK FSWGQQRTNS EQVDEDADVD 350
    TTMAALDDQP AEAQPPSPRS SSPCRTPEPD NDAHLCSSSR PCSPVHHHEG 400
    HAKLSSSPPR ASPVRMAPSY VLKKAQVLSA GSRRRKEQRY RSVISDIFDG 450
    SILSLVQCLT CDRVSATVET FQDLSLPIPG KEDLAKLHSA IYQNVPAKPG 500
    TCGDSYAAQG WLAFIVEYIR RFVVSCTPSW FWGPVVTLED CLAAFFAADE 550
    LKGDNMYSCE RCKKLRNGVK YCKVLRLPEI LCIHLKRFRH EVMYSFKINS 600
    HVSFPLEGLD LRPFLAKECT SQITTYDLLS VICHHGTAGS GHYIAYCQNV 650
    INGQWYEFDD QYVTEVHETV VQNAEGYVLF YRKSSEEAVR ERQQVVSLAA 700
    MREPSLLRFY VSREWLNKFN TFAEPGPITN QTFLCSHGGI PPHKYHYIDD 750
    LVVILPQNVW EHLYNRFGGG PAVNHLYVCS ICQVEIEALA KRRRIEIDTF 800
    IKLNKAFQAE ESPGIIYCIS MQWFREWEAF VKGKDNEPPG PIDNSRIAQV 850
    KGSGHVQLKQ GADYGQISEE TWTYLNSLYG GGPEIAIRQS VAQPLGPESL 900
    HGEQKIEAEA RAV 913
    Length:913
    Mass (Da):101,851
    Last modified:December 21, 2004 - v1
    Checksum:i54CA311FD1D3E626
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR860704 mRNA. Translation: CAH92820.1.
    RefSeqiNP_001126647.1. NM_001133175.1.

    Genome annotation databases

    GeneIDi100173645.
    KEGGipon:100173645.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR860704 mRNA. Translation: CAH92820.1 .
    RefSeqi NP_001126647.1. NM_001133175.1.

    3D structure databases

    ProteinModelPortali Q5R5Z6.
    SMRi Q5R5Z6. Positions 5-97.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100173645.
    KEGGi pon:100173645.

    Organism-specific databases

    CTDi 10868.

    Phylogenomic databases

    HOGENOMi HOG000286031.
    HOVERGENi HBG054196.
    InParanoidi Q5R5Z6.
    KOi K11848.

    Family and domain databases

    Gene3Di 3.30.2230.10. 2 hits.
    3.30.40.10. 1 hit.
    InterProi IPR006615. Pept_C19_DUSP.
    IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR001607. Znf_UBP.
    [Graphical view ]
    Pfami PF06337. DUSP. 1 hit.
    PF00443. UCH. 1 hit.
    PF02148. zf-UBP. 1 hit.
    [Graphical view ]
    SMARTi SM00695. DUSP. 2 hits.
    [Graphical view ]
    SUPFAMi SSF143791. SSF143791. 2 hits.
    PROSITEi PS51283. DUSP. 2 hits.
    PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    PS50271. ZF_UBP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. The German cDNA consortium
      Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain cortex.

    Entry informationi

    Entry nameiUBP20_PONAB
    AccessioniPrimary (citable) accession number: Q5R5Z6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 2009
    Last sequence update: December 21, 2004
    Last modified: October 1, 2014
    This is version 59 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3