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Q5R5J1

- PCKGC_PONAB

UniProt

Q5R5J1 - PCKGC_PONAB

Protein

Phosphoenolpyruvate carboxykinase, cytosolic [GTP]

Gene

PPCK1

Organism
Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 1 (21 Dec 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.By similarity

    Catalytic activityi

    GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.

    Cofactori

    Binds 1 manganese ion per subunit.By similarity

    Enzyme regulationi

    Enzyme activity is enhanced by acetylation.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei87 – 871SubstrateBy similarity
    Binding sitei237 – 2371Substrate; via amide nitrogenBy similarity
    Metal bindingi244 – 2441ManganeseBy similarity
    Binding sitei244 – 2441SubstrateBy similarity
    Metal bindingi264 – 2641Manganese; via tele nitrogenBy similarity
    Binding sitei286 – 2861SubstrateBy similarity
    Active sitei288 – 2881By similarity
    Metal bindingi311 – 3111ManganeseBy similarity
    Binding sitei405 – 4051GTPBy similarity
    Binding sitei436 – 4361GTPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi287 – 2926GTPBy similarity
    Nucleotide bindingi530 – 5334GTPBy similarity

    GO - Molecular functioni

    1. GTP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-EC

    GO - Biological processi

    1. gluconeogenesis Source: UniProtKB-UniPathway
    2. internal protein amino acid acetylation Source: UniProtKB

    Keywords - Molecular functioni

    Decarboxylase, Lyase

    Keywords - Biological processi

    Gluconeogenesis

    Keywords - Ligandi

    GTP-binding, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00138.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoenolpyruvate carboxykinase, cytosolic [GTP] (EC:4.1.1.32)
    Short name:
    PEPCK-C
    Gene namesi
    Name:PPCK1
    OrganismiPongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
    Taxonomic identifieri9601 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaePongo
    ProteomesiUP000001595: Unplaced

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 622622Phosphoenolpyruvate carboxykinase, cytosolic [GTP]PRO_0000103629Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei70 – 701N6-acetyllysine; by p300/EP300By similarity
    Modified residuei71 – 711N6-acetyllysine; by p300/EP300By similarity
    Modified residuei594 – 5941N6-acetyllysine; by p300/EP300By similarity

    Post-translational modificationi

    Lysine acetylation by p300/EP300 is increased on high glucose conditions and promotes ubiquitination by UBR5, acetylation is enhanced in the presence of BAG6. Deacetylated by SIRT2 By similarity.By similarity
    Ubiquitination by UBR5 leads to proteasomal degradation.By similarity

    Keywords - PTMi

    Acetylation, Ubl conjugation

    Interactioni

    Subunit structurei

    Monomer.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5R5J1.
    SMRiQ5R5J1. Positions 10-622.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni403 – 4053Substrate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    HOVERGENiHBG053651.
    InParanoidiQ5R5J1.
    KOiK01596.

    Family and domain databases

    Gene3Di3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPiMF_00452. PEPCK_GTP.
    InterProiIPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view]
    PANTHERiPTHR11561. PTHR11561. 1 hit.
    PfamiPF00821. PEPCK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMiSSF68923. SSF68923. 1 hit.
    PROSITEiPS00505. PEPCK_GTP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5R5J1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPPQLQNGLN LSAKVVQGSL DSLPQAVRKF LENNAELCQP DHIHICDGSE    50
    EENGRLLGQM EEEGILRRLK KYDNCWLALT DPRDVARIGS KTVIVTQEQR 100
    DTVPIPKTGL SQLGRWMSEE DFEKALNARF PGCMKGRTMY VIPFSMGPLG 150
    SPLSKIGIEL TDSPYVVASM RIMTRMGTPV LEALGDGEFV KCLHSVGCPL 200
    PLQKPLVNNW PCNPELTLIA HLPDRREIIS FGSGYGGNSL LGKKCFALRM 250
    ASRLAKEEGW LAEHMLILGI TNPEGEKKYL AAAFPSACGK TNLAMMNPSL 300
    PGWKVECVGD DIAWMKFDAQ GHLRAINPEN GFFGVAPGTS VKTNPNAIKT 350
    IQKNTIFTNV AETSDGGVYW EGIDEPLASG VTITSWKNKE WSPEDGEPCA 400
    HPNSRFCTPA SQCPIIDAAW ESPEGVPIEG IIFGGRRPAG VPLVYEALSW 450
    QHGVFVGAAM RSEATAAAEH KGKIIMHDPF AMRPFFGYNF GKYLAHWLSM 500
    AQHPAAKLPK IFHVNWFRKD KEGKFLWPGF GENSRVLEWM FNRIDGKAGA 550
    KLTPIGYIPK EDALNLKGLG HINVMELFSI SKEFWEKEVE DIEKYLEDQV 600
    NADLPCEIER EILALKQRIS QM 622
    Length:622
    Mass (Da):69,019
    Last modified:December 21, 2004 - v1
    Checksum:i1776D83AA62103E2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR860867 mRNA. Translation: CAH92975.1.
    RefSeqiNP_001126758.1. NM_001133286.1.

    Genome annotation databases

    GeneIDi100173760.
    KEGGipon:100173760.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR860867 mRNA. Translation: CAH92975.1 .
    RefSeqi NP_001126758.1. NM_001133286.1.

    3D structure databases

    ProteinModelPortali Q5R5J1.
    SMRi Q5R5J1. Positions 10-622.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100173760.
    KEGGi pon:100173760.

    Organism-specific databases

    CTDi 5105.

    Phylogenomic databases

    HOVERGENi HBG053651.
    InParanoidi Q5R5J1.
    KOi K01596.

    Enzyme and pathway databases

    UniPathwayi UPA00138 .

    Family and domain databases

    Gene3Di 3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPi MF_00452. PEPCK_GTP.
    InterProi IPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view ]
    PANTHERi PTHR11561. PTHR11561. 1 hit.
    Pfami PF00821. PEPCK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMi SSF68923. SSF68923. 1 hit.
    PROSITEi PS00505. PEPCK_GTP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. The German cDNA consortium
      Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney.

    Entry informationi

    Entry nameiPCKGC_PONAB
    AccessioniPrimary (citable) accession number: Q5R5J1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 25, 2005
    Last sequence update: December 21, 2004
    Last modified: October 1, 2014
    This is version 64 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3