SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q5R5J1

- PCKGC_PONAB

UniProt

Q5R5J1 - PCKGC_PONAB

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
Gene
PPCK1
Organism
Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle By similarity.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Binds 1 manganese ion per subunit By similarity.UniRule annotation

Enzyme regulationi

Enzyme activity is enhanced by acetylation By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei87 – 871Substrate By similarity
Binding sitei237 – 2371Substrate; via amide nitrogen By similarity
Metal bindingi244 – 2441Manganese By similarity
Binding sitei244 – 2441Substrate By similarity
Metal bindingi264 – 2641Manganese; via tele nitrogen By similarity
Binding sitei286 – 2861Substrate By similarity
Active sitei288 – 2881 By similarity
Metal bindingi311 – 3111Manganese By similarity
Binding sitei405 – 4051GTP By similarity
Binding sitei436 – 4361GTP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi287 – 2926GTP By similarity
Nucleotide bindingi530 – 5334GTP By similarity

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-EC

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-UniPathway
  2. internal protein amino acid acetylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase, cytosolic [GTP] (EC:4.1.1.32)
Short name:
PEPCK-C
Gene namesi
Name:PPCK1
OrganismiPongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Taxonomic identifieri9601 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaePongo
ProteomesiUP000001595: Unplaced

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 622622Phosphoenolpyruvate carboxykinase, cytosolic [GTP]UniRule annotation
PRO_0000103629Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei70 – 701N6-acetyllysine; by p300/EP300 By similarity
Modified residuei71 – 711N6-acetyllysine; by p300/EP300 By similarity
Modified residuei594 – 5941N6-acetyllysine; by p300/EP300 By similarity

Post-translational modificationi

Lysine acetylation by p300/EP300 is increased on high glucose conditions and promotes ubiquitination by UBR5, acetylation is enhanced in the presence of BAG6. Deacetylated by SIRT2 By similarity.UniRule annotation
Ubiquitination by UBR5 leads to proteasomal degradation By similarity.UniRule annotation

Keywords - PTMi

Acetylation, Ubl conjugation

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliQ5R5J1.
SMRiQ5R5J1. Positions 10-622.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni403 – 4053Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

HOVERGENiHBG053651.
InParanoidiQ5R5J1.
KOiK01596.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5R5J1-1 [UniParc]FASTAAdd to Basket

« Hide

MPPQLQNGLN LSAKVVQGSL DSLPQAVRKF LENNAELCQP DHIHICDGSE    50
EENGRLLGQM EEEGILRRLK KYDNCWLALT DPRDVARIGS KTVIVTQEQR 100
DTVPIPKTGL SQLGRWMSEE DFEKALNARF PGCMKGRTMY VIPFSMGPLG 150
SPLSKIGIEL TDSPYVVASM RIMTRMGTPV LEALGDGEFV KCLHSVGCPL 200
PLQKPLVNNW PCNPELTLIA HLPDRREIIS FGSGYGGNSL LGKKCFALRM 250
ASRLAKEEGW LAEHMLILGI TNPEGEKKYL AAAFPSACGK TNLAMMNPSL 300
PGWKVECVGD DIAWMKFDAQ GHLRAINPEN GFFGVAPGTS VKTNPNAIKT 350
IQKNTIFTNV AETSDGGVYW EGIDEPLASG VTITSWKNKE WSPEDGEPCA 400
HPNSRFCTPA SQCPIIDAAW ESPEGVPIEG IIFGGRRPAG VPLVYEALSW 450
QHGVFVGAAM RSEATAAAEH KGKIIMHDPF AMRPFFGYNF GKYLAHWLSM 500
AQHPAAKLPK IFHVNWFRKD KEGKFLWPGF GENSRVLEWM FNRIDGKAGA 550
KLTPIGYIPK EDALNLKGLG HINVMELFSI SKEFWEKEVE DIEKYLEDQV 600
NADLPCEIER EILALKQRIS QM 622
Length:622
Mass (Da):69,019
Last modified:December 21, 2004 - v1
Checksum:i1776D83AA62103E2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR860867 mRNA. Translation: CAH92975.1.
RefSeqiNP_001126758.1. NM_001133286.1.

Genome annotation databases

GeneIDi100173760.
KEGGipon:100173760.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR860867 mRNA. Translation: CAH92975.1 .
RefSeqi NP_001126758.1. NM_001133286.1.

3D structure databases

ProteinModelPortali Q5R5J1.
SMRi Q5R5J1. Positions 10-622.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 100173760.
KEGGi pon:100173760.

Organism-specific databases

CTDi 5105.

Phylogenomic databases

HOVERGENi HBG053651.
InParanoidi Q5R5J1.
KOi K01596.

Enzyme and pathway databases

UniPathwayi UPA00138 .

Family and domain databases

Gene3Di 3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPi MF_00452. PEPCK_GTP.
InterProi IPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view ]
PANTHERi PTHR11561. PTHR11561. 1 hit.
Pfami PF00821. PEPCK. 1 hit.
[Graphical view ]
PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMi SSF68923. SSF68923. 1 hit.
PROSITEi PS00505. PEPCK_GTP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. The German cDNA consortium
    Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.

Entry informationi

Entry nameiPCKGC_PONAB
AccessioniPrimary (citable) accession number: Q5R5J1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: December 21, 2004
Last modified: April 16, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi