Q5R5F3 (DHSO_PONAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sorbitol dehydrogenase
EC=1.1.1.14

Alternative name(s):
L-iditol 2-dehydrogenase

Gene names

Name:

SORD

Organism

Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii) [Reference proteome]

Taxonomic identifier

9601 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Ponginae › Pongo

Protein attributes

Sequence length	357 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Converts sorbitol to fructose. Part of the polyol pathway that plays an important role in sperm physiology. May play a role in the sperm motility by providing an energetic source for sperm By similarity.
Catalytic activity	L-iditol + NAD+ = L-sorbose + NADH.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Homotetramer By similarity.
Subcellular location	Mitochondrion membrane; Peripheral membrane protein. Cell projection › cilium › flagellum. Note: Associated with mitochondria of the midpiece and near the plasma membrane in the principal piece of the flagellum. Also found in the epididymosome, secreted by the epididymal epithelium and that transfers proteins from the epididymal fluid to the sperm surface By similarity.
Sequence similarities	Belongs to the zinc-containing alcohol dehydrogenase family.

Ontologies

Keywords
Cellular component	Cell projection Cilium Flagellum Membrane Mitochondrion
Ligand	Metal-binding NAD Zinc
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	sperm motility Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	mitochondrial membrane Inferred from electronic annotation. Source: UniProtKB-SubCell motile cilium Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	L-iditol 2-dehydrogenase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 357	356	Sorbitol dehydrogenase		PRO_0000301685
Sites
	Metal binding	45	1	Zinc; catalytic By similarity
	Metal binding	70	1	Zinc; catalytic By similarity
	Metal binding	71	1	Zinc; catalytic By similarity
	Binding site	51	1	Substrate By similarity
	Binding site	156	1	Substrate By similarity
	Binding site	299	1	Substrate By similarity
	Binding site	300	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5R5F3 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: 630FB00DE09BC886	FASTA	357	38,212
	10 20 30 40 50 60 MAAAAKPSNL SLVVHGPGDL RLENYPIPEP GPNEVLLRMH SVGICGSDVH YWEDGRIGNF 70 80 90 100 110 120 IVKKPMVLGH EASGTVEKVG SLVKHLKPGD RVAIEPGAPR ENDEFCKIGR YNLSPSIFFC 130 140 150 160 170 180 ATPPDDGNLC RFYKHNAAFC YKLPDNVTFE EGAMIEPLSV GIHACRRGGV TLGHKVLVCG 190 200 210 220 230 240 AGPIGMVTLL VAKAMGAAQV VVTDLSATRL SKAKEIGADL VLQISKESPQ EIARKVEGLL 250 260 270 280 290 300 GCKPEVTIEC TGAGASIQAG IYATHSGGTL VLVGLGSEMT TIPLLHAAIR EVDIKGVFRY 310 320 330 340 350 CNTWPVAISM LASKSVNVKP LITHRFPLEK ALEAFETFKK GLGLKIMLKC DPNDQNP

References

[1]	The German cDNA consortium Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR860908 mRNA. Translation: CAH93013.1.
RefSeq	NP_001126780.1. NM_001133308.1.
UniGene	Pab.18242.
3D structure databases
HSSP	HSSP built from PDB template 1PL8 based on UniProtKB Q00796.
ProteinModelPortal	Q5R5F3.
SMR	Q5R5F3. Positions 2-357.
ModBase	Search...
Proteomic databases
PRIDE	Q5R5F3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100173784.
KEGG	pon:100173784.
Organism-specific databases
CTD	6652.
Phylogenomic databases
HOVERGEN	HBG005484.
InParanoid	Q5R5F3.
KO	K00008.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
InterPro	IPR013149. ADH_C. IPR013154. ADH_GroES-like. IPR002085. ADH_SF_Zn-type. IPR002328. ADH_Zn_CS. IPR011032. GroES-like. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR11695. PTHR11695. 1 hit.
Pfam	PF08240. ADH_N. 1 hit. PF00107. ADH_zinc_N. 1 hit. [Graphical view]
SUPFAM	SSF50129. GroES_like. 1 hit.
PROSITE	PS00059. ADH_ZINC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHSO_PONAB

Accession

Primary (citable) accession number: Q5R5F3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	December 21, 2004
Last modified:	April 3, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program