Q5R5E7 (3HIDH_PONAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-hydroxyisobutyrate dehydrogenase, mitochondrial
Short name=HIBADH
EC=1.1.1.31

Gene names

Name:

HIBADH

Organism

Pongo abelii (Sumatran orangutan)

Taxonomic identifier

9601 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH.
Subunit structure	Homodimer By similarity.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the 3-hydroxyisobutyrate dehydrogenase family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	pentose-phosphate shunt Inferred from electronic annotation. Source: InterPro valine metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-hydroxyisobutyrate dehydrogenase activity Inferred from electronic annotation. Source: EC NAD binding Inferred from electronic annotation. Source: InterPro phosphogluconate dehydrogenase (decarboxylating) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 36	36	Mitochondrion By similarity
	Chain	37 – 336	300	3-hydroxyisobutyrate dehydrogenase, mitochondrial		PRO_0000290341
Regions
	Nucleotide binding	40 – 69	30	NAD By similarity
	Nucleotide binding	103 – 104	2	NAD By similarity
Sites
	Active site	209	1	By similarity
	Binding site	108	1	NAD By similarity
	Binding site	134	1	NAD; via amide nitrogen By similarity
	Binding site	284	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5R5E7 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: DA243D775F84AF48	FASTA	336	35,299
	10 20 30 40 50 60 MAASLRLLGA ASGLRYWSRR LRPAAGSFAA VCSRSVASKT PVGFIGLGNM GNPMAKNLMK 70 80 90 100 110 120 HGYPLIIYDV FPDACKEFQD AGEQVVSSPA DVAEKADRII TMLPTSINAI EAYSGANGIL 130 140 150 160 170 180 KKVKKGSLLI DSSTIDPAVS KELAKEVEKM GAVFMDAPVS GGVGAARSGN LTFMVGGVED 190 200 210 220 230 240 EFAAAQELLG CMGSNVVYCG AVGTGQAAKI CNNMLLAISM IGTAEAMNLG IRLGLDPKLL 250 260 270 280 290 300 AKILNMSSGR CWSSDTYNPV PGVMDGVPSA NNYQGGFGAT LMAKDLGLAQ DSATSTKSPI 310 320 330 LLGSLAHQIY RMMCAKGYSK KDFSSVFQFL REEETF

References

[1]	The German cDNA consortium Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR860914 mRNA. Translation: CAH93019.1.
RefSeq	NP_001127621.1. NM_001134149.1.
UniGene	Pab.18605.
3D structure databases
HSSP	HSSP built from PDB template 2GF2 based on UniProtKB P31937.
ProteinModelPortal	Q5R5E7.
SMR	Q5R5E7. Positions 41-335.
ModBase	Search...
Proteomic databases
PRIDE	Q5R5E7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSPPYT00000020615; ENSPPYP00000019832; ENSPPYG00000017693.
GeneID	100174700.
KEGG	pon:100174700.
Organism-specific databases
CTD	11112.
Phylogenomic databases
HOVERGEN	HBG050424.
InParanoid	Q5R5E7.
OMA	PMARNLI.
OrthoDB	EOG476K0N.
Family and domain databases
InterPro	IPR002204. 3-OH-isobutyrate_DH-rel_CS. IPR015815. 3hydroxyacid_DH/Rdtase. IPR008927. 6-PGluconate_DH_C-like. IPR006115. 6PGDH_NADP-bd. IPR013328. DH_multihelical. IPR011548. IsoBut3OH_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
KO	K00020.
PANTHER	PTHR22981. 3hydroxy_acid_DH. 1 hit.
Pfam	PF03446. NAD_binding_2. 1 hit. [Graphical view]
SUPFAM	SSF48179. 6DGDH_C_like. 1 hit.
TIGRFAMs	TIGR01692. HIBADH. 1 hit.
PROSITE	PS00895. 3_HYDROXYISOBUT_DH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

3HIDH_PONAB

Accession

Primary (citable) accession number: Q5R5E7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 12, 2007
Last sequence update:	December 21, 2004
Last modified:	November 16, 2011
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program