Reviewed, UniProtKB/Swiss-Prot Q5R558 (STALP_PONAB)

Names and origin

Protein names

Recommended name:
    AMSH-like protease
      Short name=AMSH-LP
    EC=3.1.2.15
Alternative name(s):
    STAM-binding protein-like 1

Gene names

Name:	STAMBPL1
Synonyms:	AMSHLP

Organism

Pongo abelii (Sumatran orangutan)

Taxonomic identifier

9601 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo

Protein attributes

Sequence length	436 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Zinc metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Does not cleave 'Lys-48'-linked polyubiquitin chains By similarity.
Catalytic activity	Ubiquitin C-terminal thioester + H2O = ubiquitin + a thiol.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Domain	The JAMM motif is essential for the protease activity By similarity.
Sequence similarities	Belongs to the peptidase M67 family. Contains 1 MPN (JAB/Mov34) domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metallopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin thiolesterase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 436	436	AMSH-like protease		PRO_0000194876
Regions
	Domain	264 – 373	110	MPN
	Motif	347 – 360	14	JAMM motif
Sites
	Metal binding	347	1	Zinc 1; catalytic By similarity
	Metal binding	349	1	Zinc 1; catalytic By similarity
	Metal binding	360	1	Zinc 1; catalytic By similarity
	Metal binding	362	1	Zinc 2 By similarity
	Metal binding	402	1	Zinc 2 By similarity
	Metal binding	408	1	Zinc 2 By similarity
	Metal binding	410	1	Zinc 2 By similarity
	Site	292	1	Indirect Zinc-binding By similarity
Amino acid modifications
	Modified residue	25	1	Phosphoserine By similarity
	Modified residue	242	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5R558-1 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: DED87DF5062D66EA	FASTA	436	49,740
	10 20 30 40 50 60 MDQPFTVNSL KKLAAMPDHT DISLSPEERV RALSKLGCNV TISEDITPRR YFRSGVEMER 70 80 90 100 110 120 MASVYLEEGN LENAFVLYNK FITLFVEKLP NHRDYQQCAV PEKQDIMKKL KEIAFPRTDE 130 140 150 160 170 180 LKNDLLKKYN VEYQEYLQSK NQYKAEILKK LEHQRLIEAE RKRIAQMRQQ QLESEQFLFF 190 200 210 220 230 240 EDQLKKQELA RGQMRSQQTS GLSEQIDGSA LSCFSTHQNN SLLNVFADQP NKSDATNYAS 250 260 270 280 290 300 HSPPVNRALT PAATLSAVQN LVVEGLRCVV LPKDLCHKFL QLAESNTVRG IETCGILCGK 310 320 330 340 350 360 LTHNEFTITH VIVPKQSAGP DYCDVENVEE LFNVQDQHDL LTLGWIHTHP TQTAFLSSVD 370 380 390 400 410 420 LHTHCSYQLM LSEAIAIVCS PKHKDTGIFR LTNAGMLEVS ACKKKGFHPH TKEPRLFSIC 430 KHVLVKDIKI IVLDLR

References

[1]	The German cDNA consortium Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain cortex.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR861012 mRNA. Translation: CAH93108.1.
RefSeq	NP_001126841.1.
UniGene	Pab.3369
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	M67.003.
Genome annotation databases
GeneID	100173848.
Organism-specific databases
CTD	100173848.
Phylogenomic databases
HOVERGEN	Q5R558.
Enzyme and pathway databases
BRENDA	3.1.2.15. 269192.
Family and domain databases
InterPro	IPR000555. Mov34_MPN_PAD1. [Graphical view]
Pfam	PF01398. Mov34. 1 hit. [Graphical view]
SMART	SM00232. JAB_MPN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

STALP_PONAB

Accession

Primary (citable) accession number: Q5R558

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	December 21, 2004
Last modified:	September 1, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)