Q5R544 (C1R_PONAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Complement C1r subcomponent
EC=3.4.21.41

Alternative name(s):
Complement component 1 subcomponent r

Cleaved into the following 2 chains:

1. Complement C1r subcomponent heavy chain
2. Complement C1r subcomponent light chain

Gene names

Name:

C1R

Organism

Pongo abelii (Sumatran orangutan)

Taxonomic identifier

9601 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo

Protein attributes

Sequence length	705 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	C1r B chain is a serine protease that combines with C1q and C1s to form C1, the first component of the classical pathway of the complement system By similarity.
Catalytic activity	Selective cleavage of Lys(or Arg)-|-Ile bond in complement subcomponent C1s to form the active form of C1s (EC 3.4.21.42).
Subunit structure	C1 is a calcium-dependent trimolecular complex of C1q, C1r and C1s in the molar ration of 1:2:2. C1r is a dimer of identical chains, each of which is activated by cleavage into two chains, A and B, connected by disulfide bonds By similarity.
Post-translational modification	The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains By similarity.
Sequence similarities	Belongs to the peptidase S1 family. Contains 2 CUB domains. Contains 1 EGF-like domain. Contains 1 peptidase S1 domain. Contains 2 Sushi (CCP/SCR) domains.

Ontologies

Keywords
Biological process	Complement pathway Immunity Innate immunity
Domain	EGF-like domain Repeat Signal Sushi
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Hydroxylation Phosphoprotein
Gene Ontology (GO)
Biological process	complement activation, classical pathway Inferred from electronic annotation. Source: UniProtKB-KW innate immune response Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: InterPro
Molecular function	calcium ion binding Inferred from electronic annotation. Source: InterPro serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	By similarity
	Chain	18 – 705	688	Complement C1r subcomponent		PRO_0000042190
	Chain	18 – 463	446	Complement C1r subcomponent heavy chain By similarity		PRO_0000042191
	Chain	464 – 705	242	Complement C1r subcomponent light chain By similarity		PRO_0000042192
Regions
	Domain	18 – 141	124	CUB 1
	Domain	142 – 190	49	EGF-like; calcium-binding Potential
	Domain	193 – 305	113	CUB 2
	Domain	307 – 373	67	Sushi 1
	Domain	374 – 449	76	Sushi 2
	Domain	464 – 702	239	Peptidase S1
Sites
	Active site	502	1	Charge relay system By similarity
	Active site	557	1	Charge relay system By similarity
	Active site	654	1	Charge relay system By similarity
Amino acid modifications
	Modified residue	167	1	(3R)-3-hydroxyasparagine By similarity
	Modified residue	206	1	Phosphoserine; by CK2 By similarity
	Glycosylation	125	1	N-linked (GlcNAc...) Potential
	Glycosylation	221	1	N-linked (GlcNAc...) Potential
	Glycosylation	514	1	N-linked (GlcNAc...) Potential
	Glycosylation	581	1	N-linked (GlcNAc...) Potential
	Disulfide bond	71 ↔ 89		By similarity
	Disulfide bond	146 ↔ 165		By similarity
	Disulfide bond	161 ↔ 174		By similarity
	Disulfide bond	176 ↔ 189		By similarity
	Disulfide bond	193 ↔ 220		By similarity
	Disulfide bond	250 ↔ 268		By similarity
	Disulfide bond	309 ↔ 358		By similarity
	Disulfide bond	338 ↔ 371		By similarity
	Disulfide bond	376 ↔ 429		By similarity
	Disulfide bond	406 ↔ 447		By similarity
	Disulfide bond	451 ↔ 577		Interchain (between heavy and light chains) By similarity
	Disulfide bond	620 ↔ 639		By similarity
	Disulfide bond	650 ↔ 680		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5R544 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: 4DFA4080BB75174B	FASTA	705	80,064
	10 20 30 40 50 60 MWLLYLLVPA MFCRAGGSIP IPQKLFGEVT SPLFPKPYPN NFETTTVITV PTGYRVKLVF 70 80 90 100 110 120 QQFDLEPSEG CFYDYVKISA DKKSLGRFCG QLGSPLGNPP GKKEFMSQGN KMLLTFHTDF 130 140 150 160 170 180 SNEENGTIMF YKGFLAYYQA VDLDECASQS KSGEEDPQPQ CQHLCHNYVG GYFCSCLPGY 190 200 210 220 230 240 ELQKDRHSCQ AECSSELYTE ASGYISSLEY PRSYPPDLRC NYSIRVERGL TLHLKFLEPF 250 260 270 280 290 300 EIDDHQQVHC PYDQLQIYAN GKNIGEFCGK QRPPDLDTSS NAVDLLFFTD ESGDSRGWNL 310 320 330 340 350 360 RYTTEIIKCP QPKTLDEFTI IQNLQPQYQF RDYFIATCKQ GYQLIEGNQV LHSFTAVCQD 370 380 390 400 410 420 DGTWHRAMPR CKIKDCGQPR NLPNGAFRYT TTMGVNTYKA RIQYYCHKPY YKMQTRAGSR 430 440 450 460 470 480 ESEQGVYTCT AQGIWKNEQK GEKIPRCLPV CGKPVNPVEQ RQRIIGGQKA KMGNFPWQVF 490 500 510 520 530 540 TNIHGRGGGA LLGDRWILTA AHTLYPKEHE AQTNASLDVF LGHTNVEELM KLGNHPIRRV 550 560 570 580 590 600 SVHPDYRQDE SHNFEGDIAL LELENSVTLG PNLLPICLPD NETFYDLGLM GYVSGFGVME 610 620 630 640 650 660 EKIAHDLRFV RLPVANPQAC ETWLRGKNRM DVFSQNMFCA GHPSLKQDAC QGDSGGVFAV 670 680 690 700 RDPNTDRWVA TGIVSWGIGC SRGYGFYTKV LNYVDWIKKE MEEED

References

[1]	The German cDNA consortium Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR861030 mRNA. Translation: CAH93122.1.
RefSeq	NP_001126847.1. NM_001133375.1.
UniGene	Pab.18568.
3D structure databases
ProteinModelPortal	Q5R544.
SMR	Q5R544. Positions 140-192, 307-703.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100173855.
KEGG	pon:100173855.
Organism-specific databases
CTD	715.
Phylogenomic databases
HOVERGEN	HBG000559.
InParanoid	Q5R544.
Family and domain databases
InterPro	IPR016060. Complement_control_module. IPR000859. CUB. IPR001881. EGF-like_Ca-bd. IPR013032. EGF-like_reg_CS. IPR000152. EGF-type_Asp/Asn_hydroxyl_site. IPR018097. EGF_Ca-bd_CS. IPR009003. Pept_cys/ser_Trypsin-like. IPR024175. Pept_S1A_C1r/C1S/mannan-bd. IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. IPR000436. Sushi_SCR_CCP. [Graphical view]
Gene3D	G3DSA:2.10.70.10. Complement_control_module. 2 hits. G3DSA:2.60.120.290. CUB. 2 hits.
KO	K01330.
Pfam	PF00431. CUB. 2 hits. PF07645. EGF_CA. 1 hit. PF00084. Sushi. 2 hits. PF00089. Trypsin. 1 hit. [Graphical view]
PIRSF	PIRSF001155. C1r_C1s_MASP. 1 hit.
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00032. CCP. 2 hits. SM00042. CUB. 2 hits. SM00179. EGF_CA. 1 hit. SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF57535. Complement_control_module. 2 hits. SSF49854. CUB. 2 hits. SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS00010. ASX_HYDROXYL. 1 hit. PS01180. CUB. 2 hits. PS00022. EGF_1. False negative. PS01186. EGF_2. 1 hit. PS50026. EGF_3. False negative. PS01187. EGF_CA. 1 hit. PS50923. SUSHI. 2 hits. PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. False negative. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C1R_PONAB

Accession

Primary (citable) accession number: Q5R544

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2005
Last sequence update:	December 21, 2004
Last modified:	November 16, 2011
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program