Q5R514 (UMPS_PONAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uridine 5'-monophosphate synthase
Short name=UMP synthase

Including the following 2 domains:

1. Orotate phosphoribosyltransferase
   Short name=OPRTase
   EC=2.4.2.10
2. Orotidine 5'-phosphate decarboxylase
   EC=4.1.1.23
   Alternative name(s):
   OMPdecase

Gene names

Name:

UMPS

Organism

Pongo abelii (Sumatran orangutan)

Taxonomic identifier

9601 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo

Protein attributes

Sequence length	480 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate. Orotidine 5'-phosphate = UMP + CO2.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 2/2.
Subunit structure	Homodimer By similarity.
Sequence similarities	In the N-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family. In the C-terminal section; belongs to the OMP decarboxylase family.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Molecular function	Decarboxylase Glycosyltransferase Lyase Transferase
PTM	Acetylation Phosphoprotein
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	'de novo' UMP biosynthetic process Inferred from electronic annotation. Source: InterPro 'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	orotate phosphoribosyltransferase activity Inferred from electronic annotation. Source: EC orotidine-5'-phosphate decarboxylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 480	479	Uridine 5'-monophosphate synthase		PRO_0000257814
Regions
	Region	2 – 214	213	OPRTase
	Region	215 – 220	6	Domain linker
	Region	221 – 480	260	OMPdecase
Sites
	Active site	314	1	By similarity
	Active site	314	1	For OMPdecase activity By similarity
	Active site	317	1	For OMPdecase activity By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	37	1	Phosphotyrosine By similarity
	Modified residue	162	1	N6-acetyllysine By similarity
	Modified residue	214	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5R514 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: 18DBEA8FCF5DB6BC	FASTA	480	52,128
	10 20 30 40 50 60 MAAVGAALGP LVTGLYDVQA FKFGDFVLKS GLSSPIYIDL RGIVSRPRLL SQVADTLFQT 70 80 90 100 110 120 AQNAGISFDT VCGVPYTALP LATVICSTNQ IPMLIRRKET KDYGTKRLVE GTINPGETCL 130 140 150 160 170 180 IIEDVVTSGS SVLETAEVLQ KEGLKVTDAI VLLDREQGGK DKLQAHGIRL HSVCTLSKML 190 200 210 220 230 240 EILEQQKKID AETVGRVKRF IQENVFVAAN HNGSPLSIKE APKELSFSAR AELPRIHPVA 250 260 270 280 290 300 SKLLRLMQKK ETNLCLSADV SEARELLQLA DALGPSICML KTHVDILNDF TLDVMKELIT 310 320 330 340 350 360 LAKRHEFLIF EDRKFADIGN TVKKQYEGGV FKIASWADLV NAHVVPGSGV VKGLQEVGLP 370 380 390 400 410 420 LHRGCLLIAE MSSTGSLATG DYTRAAVRMA EEHSEFVVGF ISGSRVSMKP EFLHLTPGVQ 430 440 450 460 470 480 LEAGGDNLGQ QYNSPQEVIG KRGSDIIIVG RGIISAADCL EAAEMYRKAA WEAYLSRLGV

References

[1]	The German cDNA consortium Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain cortex.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR861071 mRNA. Translation: CAH93152.1.
RefSeq	NP_001126858.1. NM_001133386.1.
UniGene	Pab.1766.
3D structure databases
HSSP	HSSP built from PDB template 1DQW based on UniProtKB P03962.
ProteinModelPortal	Q5R514.
SMR	Q5R514. Positions 221-479.
ModBase	Search...
Proteomic databases
PRIDE	Q5R514.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100173867.
KEGG	pon:100173867.
Organism-specific databases
CTD	7372.
Phylogenomic databases
HOVERGEN	HBG000870.
InParanoid	Q5R514.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR014732. OMPdecase. IPR018089. OMPdecase_AS. IPR001754. OMPdeCOase_dom. IPR004467. Or_phspho_trans_clade-1. IPR023031. Orotate_PribosylTferase. IPR000836. PRibTrfase. IPR011060. RibuloseP-bd_barrel. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K13421.
Pfam	PF00215. OMPdecase. 1 hit. PF00156. Pribosyltran. 1 hit. [Graphical view]
SMART	SM00934. OMPdecase. 1 hit. [Graphical view]
SUPFAM	SSF51366. RibP_bind_barrel. 1 hit.
TIGRFAMs	TIGR00336. PyrE. 1 hit. TIGR01740. PyrF. 1 hit.
PROSITE	PS00156. OMPDECASE. 1 hit. PS00103. PUR_PYR_PR_TRANSFER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UMPS_PONAB

Accession

Primary (citable) accession number: Q5R514

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	December 21, 2004
Last modified:	November 16, 2011
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program