Reviewed, UniProtKB/Swiss-Prot Q5R513 (MPPA_PONAB)

Names and origin

Protein names

Recommended name:
    Mitochondrial-processing peptidase subunit alpha
    EC=3.4.24.64
Alternative name(s):
    Alpha-MPP

Gene names

Name:

PMPCA

Organism

Pongo abelii (Sumatran orangutan)

Taxonomic identifier

9601 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo

Protein attributes

Sequence length	525 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Cleaves presequences (transit peptides) from mitochondrial protein precursors By similarity.
Catalytic activity	Release of N-terminal transit peptides from precursor proteins imported into the mitochondrion, typically with Arg in position P2.
Subunit structure	Heterodimer of alpha and beta subunits By similarity.
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the peptidase M16 family.
Caution	Does not seem to have a protease activity as it lack the zinc-binding site.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Molecular function	Hydrolase Metalloprotease Protease
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 33	33	Mitochondrion By similarity
	Chain	34 – 525	492	Mitochondrial-processing peptidase subunit alpha		PRO_0000045849
Experimental info
	Sequence conflict	20	1	C → S in CAH93153. Ref.1
	Sequence conflict	300	1	R → L in CAH93153. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q5R513-1 [UniParc]. Last modified January 24, 2006. Version 2. Checksum: 22B7CA78966A091C	FASTA	525	58,340
	10 20 30 40 50 60 MAAVVLAATR LLRGSGSWGC SRLRFGPPAY RRFSSGGAYP NIPLSSPLPG VPKPVFATVD 70 80 90 100 110 120 GQEKFETKVT TLDNGLRVAS QNKFGQFCTV GILINSGSRY EAKYLSGIAH FLEKLAFSST 130 140 150 160 170 180 ARFDSKDEIL LTLEKHGGIC DCQTSRDTTM YAVSADSKGL DTVVGLLADV VLQPRLTDEE 190 200 210 220 230 240 VEMTRMTVQF ELEDLNLRPD PEPLLTEMIH EAAYRENTVG LHRFCPTENI AKINREVLHS 250 260 270 280 290 300 YLRNYYTPDR MVLAGVGVEH EHLVDCARKY LLGIQPAWGS AEAVDIDRSV AQYTGGIAKR 310 320 330 340 350 360 ERDMSNVSLG PTPIPELTHI MVGLESCSFL EEDFIPFAVL NMMMGGGGSF SAGGPGKGMF 370 380 390 400 410 420 SRLYLNVLNR HHWMYNATSY HHSYEDTGLL CIHASADPRQ VREMVEIITK EFILMSGTVD 430 440 450 460 470 480 AVELERAKTQ LTSMLMMNLE SRPVIFEDVG RQVLATRSRK LPHELCTLIR NVKPEDVKRV 490 500 510 520 ASKMLRGKPA VAALGDLTDL PTYEHIQTAL SSKDGRLPRT YRLFR

References

[1]	The German cDNA consortium Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain cortex.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR859499 mRNA. Translation: CAH91668.1. CR861072 mRNA. Translation: CAH93153.1.
RefSeq	NP_001126859.1.
UniGene	Pab.18559
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	100173868.
Phylogenomic databases
HOVERGEN	Q5R513.
Enzyme and pathway databases
BRENDA	3.4.24.64. 269192.
Family and domain databases
InterPro	IPR011237. Pept_M16_core. IPR011765. Pept_M16_N. IPR001431. Pept_M16_Zn_BS. IPR007863. Peptidase_M16_C. [Graphical view]
Gene3D	G3DSA:3.30.830.10. Pept_M16_core. 2 hits.
Pfam	PF00675. Peptidase_M16. 1 hit. PF05193. Peptidase_M16_C. 1 hit. [Graphical view]
PROSITE	PS00143. INSULINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MPPA_PONAB

Accession

Primary (citable) accession number: Q5R513
Secondary accession number(s): Q5R992

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2006
Last sequence update:	January 24, 2006
Last modified:	March 3, 2009
This is version 26 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)