Q5R501 (OST48_PONAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit
Short name=DDOST 48 kDa subunit
Short name=Oligosaccharyl transferase 48 kDa subunit
EC=2.4.1.119

Gene names

Name:

DDOST

Organism

Pongo abelii (Sumatran orangutan)

Taxonomic identifier

9601 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo

Protein attributes

Sequence length	439 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Essential subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains By similarity.
Catalytic activity	Dolichyl diphosphooligosaccharide + protein L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L-asparagine.
Pathway	Protein modification; protein glycosylation.
Subunit structure	Component of the oligosaccharyltransferase (OST) complex. OST seems to exist in different forms which contain at least RPN1, RPN2, OST48, DAD1, OSTC, KRTCAP2 and either STT3A or STT3B. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes even after release from the ribosome By similarity.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type I membrane protein By similarity.
Sequence similarities	Belongs to the DDOST 48 kDa subunit family.
Sequence caution	The sequence CAH93165.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Signal Transmembrane Transmembrane helix
Molecular function	Transferase
Gene Ontology (GO)
Biological process	protein N-linked glycosylation via asparagine Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW oligosaccharyltransferase complex Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	dolichyl-diphosphooligosaccharide-protein glycotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	By similarity
	Chain	27 – 439	413	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit		PRO_0000357446
Regions
	Topological domain	27 – 410	384	Lumenal Potential
	Transmembrane	411 – 430	20	Helical; Potential
	Topological domain	431 – 439	9	Cytoplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5R501 [UniParc]. Last modified December 16, 2008. Version 2. Checksum: CF850058921A466B	FASTA	439	48,783
	10 20 30 40 50 60 MEPSTAARAW ALFWLLPPLL GAVCASGPRT LVLLDNLNVR ETHSLFFRSL KDRGFELTFK 70 80 90 100 110 120 TADDPSLSLI KYGEFLYDNL IIFSPSVEDF GGNINVETIS AFIDGGGSVL VAASSDIGDP 130 140 150 160 170 180 LRELGSECGI EFDEEKTAVI DHHNYDISDL GQHTLIVADT ENLLKAPTIV GKSSLNPILF 190 200 210 220 230 240 RGVGMVADPD NPLVLDILTG SSTSYSFFPD KPITQYPHAV GKNTLLIAGL QARNNARVIF 250 260 270 280 290 300 SGSLDFFSDS FFNSAVQKAA PGSQRYSQTG NYELAVALSR WVFKEEGVLR VGPVSHHRVG 310 320 330 340 350 360 ETAPPNAYTV TDLVEYSIVI QQLSNGKWVP FDGDDIQLEF VRIDPFVRTF LKKKGGKYSV 370 380 390 400 410 420 QFKLPDVYGV FQFKVDYNRL GYTHLYSSTQ VSVRPLQHTQ YERFIPSAYP YYASAFSMML 430 GLFIFSIVFL HMKEKEKSD

References

[1]	The German cDNA consortium Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain cortex.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR861085 mRNA. Translation: CAH93165.1. Different initiation.
UniGene	Pab.18550.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG053378.
InParanoid	Q5R501.
Family and domain databases
InterPro	IPR005013. OligosaccharylTrfase_su_Wbp1. [Graphical view]
PANTHER	PTHR10830. DDOST_48kDa. 1 hit.
Pfam	PF03345. DDOST_48kD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OST48_PONAB

Accession

Primary (citable) accession number: Q5R501

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 16, 2008
Last sequence update:	December 16, 2008
Last modified:	December 14, 2011
This is version 27 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program