Reviewed, UniProtKB/Swiss-Prot Q5R4X0 (IMPA1_PONAB)

Names and origin

Protein names

Recommended name:
    Inositol monophosphatase
    EC=3.1.3.25
Alternative name(s):
    Inositol-1(or 4)-monophosphatase
      Short name=IMPase
      Short name=IMP
    Lithium-sensitive myo-inositol monophosphatase A1

Gene names

Name:	IMPA1
Synonyms:	IMPA

Organism

Pongo abelii (Sumatran orangutan)

Taxonomic identifier

9601 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo

Protein attributes

Sequence length	277 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Responsible for the provision of inositol required for synthesis of phosphatidylinositol and polyphosphoinositides and has been implicated as the pharmacological target for lithium action in brain. Can use myo-inositol monophosphates, myo-inositol-1,3-diphosphate, myo-inositol-1,4-diphosphate, scyllo-inositol-phosphate, glucose-1-phosphate, glucose-6-phosphate, fructose-1-phosphate, beta-glycerophosphate, and 2'-AMP as substrates By similarity.
Catalytic activity	Myo-inositol phosphate + H2O = myo-inositol + phosphate.
Cofactor	Magnesium By similarity.
Enzyme regulation	Inhibited by Li+ By similarity.
Pathway	Polyol metabolism; myo-inositol biosynthesis; myo-inositol from D-glucose 6-phosphate: step 2/2.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the inositol monophosphatase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Lithium Magnesium Metal-binding
Molecular function	Hydrolase
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	inositol-1(or 4)-monophosphatase activity Inferred from electronic annotation. Source: EC lithium ion binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 277	277	Inositol monophosphatase		PRO_0000142516
Regions
	Region	92 – 95	4	Substrate binding By similarity
	Region	194 – 196	3	Substrate binding By similarity
Sites
	Metal binding	70	1	Magnesium 1 By similarity
	Metal binding	90	1	Magnesium 1 By similarity
	Metal binding	90	1	Magnesium 2 By similarity
	Metal binding	92	1	Magnesium 1; via carbonyl oxygen By similarity
	Metal binding	93	1	Magnesium 2 By similarity
	Metal binding	220	1	Magnesium 2 By similarity
	Binding site	70	1	Substrate By similarity
	Binding site	213	1	Substrate By similarity
	Binding site	220	1	Substrate By similarity
Experimental info
	Sequence conflict	109	1	I → V in CAH92554. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q5R4X0-1 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: 664C1303B0801221	FASTA	277	30,171
	10 20 30 40 50 60 MADPWQECMD YAVTLARQAG EVVCEAIKNE MNVMLKSSPV DLVTATDQKV EKMLISSIKE 70 80 90 100 110 120 KYPSHSFIGE ESVAAGEKSI LTDNPTWIID PIDGTTNFVH RFPFVAVSIG FAVNKKIEFG 130 140 150 160 170 180 VVYSCVEGKM YTARKGKGAF CNGQKLQVSQ QEDITKSLLV TELGSSRTPE TVRIVLSNME 190 200 210 220 230 240 KLFCIPVHGI RSVGTAAVNM CLVATGGADA YYEMGIHCWD VAGAGIIVTE AGGVLMDVTG 250 260 270 GPFDLMSRRV IAANNRILAE RIAKEIQVIP LQRDDED

References

[1]	The German cDNA consortium Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain cortex.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR860429 mRNA. Translation: CAH92554.1. CR861120 mRNA. Translation: CAH93196.1.
RefSeq	NP_001127563.1.
UniGene	Pab.18540
3D structure databases
SMR	Q5R4X0. Positions 5-276.
ModBase	Search...
Genome annotation databases
GeneID	100174641.
Organism-specific databases
CTD	100174641.
Phylogenomic databases
HOVERGEN	Q5R4X0.
Enzyme and pathway databases
BRENDA	3.1.3.25. 269192.
Family and domain databases
InterPro	IPR020583. Inositol_monoP_metal-BS. IPR020552. Inositol_monoPase_Li-sen. IPR000760. Inositol_monophosphatase. IPR020550. Inositol_monophosphatase_CS. [Graphical view]
PANTHER	PTHR20854. Inositol_P. 1 hit.
Pfam	PF00459. Inositol_P. 1 hit. [Graphical view]
PRINTS	PR00377. IMPHPHTASES. PR00378. LIIMPHPHTASE.
ProDom	PD023420. Inositol_P. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00629. IMP_1. 1 hit. PS00630. IMP_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IMPA1_PONAB

Accession

Primary (citable) accession number: Q5R4X0
Secondary accession number(s): Q5R6Q6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	December 21, 2004
Last modified:	September 22, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)