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Protein

Myeloid leukemia factor 1

Gene

MLF1

Organism
Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in lineage commitment of primary hemopoietic progenitors by restricting erythroid formation and enhancing myeloid formation. Interferes with erythropoietin-induced erythroid terminal differentiation by preventing cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels. Suppresses RFWD2/COP1 activity via CSN3 which activates p53 and induces cell cycle arrest. Binds DNA and affects the expression of a number of genes so may function as a transcription factor in the nucleus (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, DNA-binding
Biological processCell cycle, Differentiation

Names & Taxonomyi

Protein namesi
Recommended name:
Myeloid leukemia factor 1
Alternative name(s):
Myelodysplasia-myeloid leukemia factor 1
Gene namesi
Name:MLF1
OrganismiPongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Taxonomic identifieri9601 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaePongo
Proteomesi
  • UP000001595 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002475991 – 268Myeloid leukemia factor 1Add BLAST268

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei6PhosphoserineBy similarity1
Modified residuei8PhosphoserineBy similarity1
Modified residuei32PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylation is required for binding to YWHAZ.By similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Interacts with CENPU. Also interacts with NRBP1/MADM, YWHAZ/14-3-3-zeta and HNRPUL2/MANP. NRBP1 recruits a serine kinase which phosphorylates both itself and MLF1. Phosphorylated MLF1 then binds to YWHAZ and is retained in the cytoplasm. Retained in the nucleus by binding to HNRPUL2. Binds to COPS3/CSN3 which is required for suppression of RFWD2 and activation of p53 (By similarity).By similarity

Structurei

3D structure databases

SMRiQ5R4T3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni50 – 125Interaction with COPS3By similarityAdd BLAST76

Sequence similaritiesi

Belongs to the MLF family.Curated

Phylogenomic databases

HOVERGENiHBG019060.
InParanoidiQ5R4T3.
KOiK15622.

Family and domain databases

InterProiView protein in InterPro
IPR019376. Myeloid_leukemia_factor.
PANTHERiPTHR13105. PTHR13105. 1 hit.
PfamiView protein in Pfam
PF10248. Mlf1IP. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5R4T3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFRMLSSSFE DDPFFSESIL AHRENMRQMM RSFTEPFGRD LLSISDGRGR
60 70 80 90 100
VHNRRGHNDG EDSLTHTDVS SLQTVDQMVS NMRNYMQKLE RNFGQLSVDP
110 120 130 140 150
NGHSFCSSSV MTYSKIGDEP PKVFQASTQT RRAPGGIKET RKAMRDSDSG
160 170 180 190 200
LEKMAIGHHI HDRAHVIKKS KNKKTGDEEV NQEFINMNES DAHAFDEEWQ
210 220 230 240 250
SEVLKYKPGR HNLENTRMRS VGHENPGSRE LKRREKPQQS PAIEHGRRSD
260
VLGDKLHIKG SSVKSNKK
Length:268
Mass (Da):30,667
Last modified:December 21, 2004 - v1
Checksum:i8A5304E519D1C757
GO
Isoform 2 (identifier: Q5R4T3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-110: Missing.

Show »
Length:158
Mass (Da):18,019
Checksum:i1DA2BEFE4BA2D4B8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti257H → Y in CAH92160 (Ref. 1) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0200211 – 110Missing in isoform 2. 1 PublicationAdd BLAST110

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR860009 mRNA. Translation: CAH92160.1.
CR861160 mRNA. Translation: CAH93233.1.
RefSeqiNP_001127516.1. NM_001134044.1. [Q5R4T3-1]

Genome annotation databases

GeneIDi100174592.
KEGGipon:100174592.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMLF1_PONAB
AccessioniPrimary (citable) accession number: Q5R4T3
Secondary accession number(s): Q5R7V0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: December 21, 2004
Last modified: March 15, 2017
This is version 55 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families