Reviewed, UniProtKB/Swiss-Prot Q5R4S2 (B3GN1_PONAB)

Names and origin

Protein names

Recommended name:
    N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase
    EC=2.4.1.149
Alternative name(s):
    Poly-N-acetyllactosamine extension enzyme
    I-beta-1,3-N-acetylglucosaminyltransferase
      Short name=iGnT
    UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1

Gene names

Name:	B3GNT1
Synonyms:	B3GNT6

Organism

Pongo abelii (Sumatran orangutan)

Taxonomic identifier

9601 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo

Protein attributes

Sequence length	415 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Can initiate the synthesis or the elongation of the linear poly-N-acetyllactosaminoglycans By similarity.
Catalytic activity	UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-R.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 415	415	N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase		PRO_0000080557
Regions
	Topological domain	1 – 8	8	Cytoplasmic Potential
	Transmembrane	9 – 36	28	Signal-anchor for type II membrane protein Potential
	Topological domain	37 – 415	379	Lumenal Potential
Amino acid modifications
	Glycosylation	204	1	N-linked (GlcNAc...) Potential
	Glycosylation	300	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5R4S2-1 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: E73C1299D8F34CE2	FASTA	415	47,149
	10 20 30 40 50 60 MQMSYAIRCA FYQLLLAALM LVAMLQLLYL SLLSGLHGQE EQDQYFEFFP PSPRSVDQVK 70 80 90 100 110 120 TQLRTALASG GVLDASGDYR VYRGLLKTTM DPNDVILATH ASVDNLLHLS GLLERWEGPL 130 140 150 160 170 180 SVSVFAATKE EAQLATVLAY ALSSHCPDMR ARVAMHLVCP SRYEAAVPDP REPGEFALLR 190 200 210 220 230 240 SCQEVFDKLA RVAQPGINYA LGTNVSYPNN LLRNLAREGA NYALVIDVDM VPSEGLWRGL 250 260 270 280 290 300 REMLDQSNQW GGTALVVPAF EIRRARRMPM NKNELVQLYQ VGEVRPFYYG LCTPCQAPTN 310 320 330 340 350 360 YSRWVNLPEE SLLRPAYVVP WQDPWEPFYV AGGKVPTFDE RFRQYGFNRI SQACELHVAG 370 380 390 400 410 FDFEVLNEGF LVHKGFKEAL KFHPQKEAEN QHNKILYRQF KQELKAKYPN SPRRC

References

[1]	The German cDNA consortium Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain cortex.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR861172 mRNA. Translation: CAH93244.1.
RefSeq	NP_001126908.1.
UniGene	Pab.17541
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT49. Glycosyltransferase Family 49.
Genome annotation databases
GeneID	100173924.
Organism-specific databases
CTD	100173924.
Phylogenomic databases
HOVERGEN	Q5R4S2.
InParanoid	Q5R4S2.
Enzyme and pathway databases
BRENDA	2.4.1.149. 269192.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

B3GN1_PONAB

Accession

Primary (citable) accession number: Q5R4S2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	December 21, 2004
Last modified:	December 15, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)