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Protein

Calnexin

Gene

CANX

Organism
Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi74 – 741Calcium; via carbonyl oxygenBy similarity
Metal bindingi117 – 1171Calcium; via carbonyl oxygenBy similarity
Binding sitei164 – 1641CarbohydrateBy similarity
Binding sitei166 – 1661CarbohydrateBy similarity
Binding sitei185 – 1851CarbohydrateBy similarity
Binding sitei216 – 2161CarbohydrateBy similarity
Metal bindingi436 – 4361CalciumBy similarity

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. carbohydrate binding Source: UniProtKB-KW

GO - Biological processi

  1. clathrin-mediated endocytosis Source: UniProtKB
  2. protein folding Source: InterPro
  3. synaptic vesicle endocytosis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Ligandi

Calcium, Lectin, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Calnexin
Gene namesi
Name:CANX
OrganismiPongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Taxonomic identifieri9601 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaePongo
ProteomesiUP000001595 Componenti: Unplaced

Subcellular locationi

Endoplasmic reticulum membrane; Single-pass type I membrane protein. Endoplasmic reticulum By similarity. Melanosome By similarity
Note: The palmitoylated form preferentially localizes to the perinuclear rough ER.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini21 – 481461LumenalSequence AnalysisAdd
BLAST
Transmembranei482 – 50221HelicalSequence AnalysisAdd
BLAST
Topological domaini503 – 59290CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB
  2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  3. integral component of membrane Source: UniProtKB-KW
  4. melanosome Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence AnalysisAdd
BLAST
Chaini21 – 592572CalnexinPRO_0000004200Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei137 – 1371N6-acetyllysineBy similarity
Disulfide bondi160 ↔ 194By similarity
Disulfide bondi360 ↔ 366By similarity
Lipidationi502 – 5021S-palmitoyl cysteineBy similarity
Lipidationi503 – 5031S-palmitoyl cysteineBy similarity
Modified residuei554 – 5541PhosphoserineBy similarity
Modified residuei562 – 5621PhosphothreonineBy similarity
Modified residuei564 – 5641Phosphoserine; by MAPK3By similarity
Modified residuei583 – 5831PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated at Ser-564 by MAPK3/ERK1. phosphorylation by MAPK3/ERK1 increases its association with ribosomes (By similarity).By similarity
Palmitoylation by DHHC6 leads to the preferential localization to the perinuclear rough ER. It mediates the association of calnexin with the ribosome-translocon complex (RTC) which is required for efficient folding of glycosylated proteins (By similarity).By similarity

Keywords - PTMi

Acetylation, Disulfide bond, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PRIDEiQ5R440.

Interactioni

Subunit structurei

Interacts with MAPK3/ERK1. Interacts with KCNH2 (By similarity). Associates with ribosomes. Interacts with SGIP1; involved in negative regulation of endocytosis. The palmitoylated form interacts with the ribosome-translocon complex component SSR1, promoting efficient folding of glycoproteins (By similarity). Interacts with KCNH2 (By similarity). Interacts with SERPINA2 and with the S and Z variants of SERPINA1 (By similarity). Part of a complex which includes CANX, DERL1, DERL2, DDOST/OST48, RPN1, RPN2, SELK, STT3A, VCP AND VIMP. Interacts with PPIB (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ5R440.
SMRiQ5R440. Positions 60-457.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati278 – 290131-1Add
BLAST
Repeati295 – 307131-2Add
BLAST
Repeati314 – 326131-3Add
BLAST
Repeati333 – 345131-4Add
BLAST
Repeati348 – 358112-1Add
BLAST
Repeati367 – 377112-2Add
BLAST
Repeati381 – 391112-3Add
BLAST
Repeati395 – 405112-4Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni276 – 409134P domain (Extended arm)By similarityAdd
BLAST
Regioni278 – 345684 X approximate repeatsAdd
BLAST
Regioni326 – 35934Interaction with PPIBBy similarityAdd
BLAST
Regioni348 – 405584 X approximate repeatsAdd
BLAST
Regioni503 – 59290Sufficient to mediate interaction with SGIP1By similarityAdd
BLAST

Sequence similaritiesi

Belongs to the calreticulin family.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG005407.
InParanoidiQ5R440.
KOiK08054.

Family and domain databases

Gene3Di2.10.250.10. 1 hit.
2.60.120.200. 1 hit.
InterProiIPR001580. Calret/calnex.
IPR018124. Calret/calnex_CS.
IPR009033. Calreticulin/calnexin_P_dom.
IPR013320. ConA-like_dom.
[Graphical view]
PANTHERiPTHR11073. PTHR11073. 1 hit.
PfamiPF00262. Calreticulin. 1 hit.
[Graphical view]
PRINTSiPR00626. CALRETICULIN.
SUPFAMiSSF49899. SSF49899. 2 hits.
SSF63887. SSF63887. 1 hit.
PROSITEiPS00803. CALRETICULIN_1. 1 hit.
PS00804. CALRETICULIN_2. 1 hit.
PS00805. CALRETICULIN_REPEAT. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5R440-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGKWLLCML LVLGTAIVEA HDGHDDDVID IEDDLDDVIE EVEDSKPDTT
60 70 80 90 100
APPSSPKVTY KAPVPTGEVY FADSFDRGTL SGWILSKAKK DDTDDEIAKY
110 120 130 140 150
DGKWEVDEMK ESKLPGDKGL VLMSRAKHHA ISAKLNKPFL FDTKPLIVQY
160 170 180 190 200
EVNFQNGIEC GGAYVKLLSK TPELNLDQFH DKTPYTIMFG PDKCGEDYKL
210 220 230 240 250
HFIFRHKNPK TGIYEEKHAK RPDADLKTYF TDKKTHLYTL ILNPDNSFEI
260 270 280 290 300
LVDQSVVNSG NLLNDMTPPV NPSREIEDPE DRKPEDWDER PKIPDPEAVK
310 320 330 340 350
PDDWDEDAPA KIPDEEATKP EGWLDDEPEY VPDPDAEKPE DWDEDMDGEW
360 370 380 390 400
EAPQIANPKC ESAPGCGVWQ RPMIDNPNYK GKWKPPMIDN PSYQGIWKPR
410 420 430 440 450
KIPNPDFFED LEPFRMTPFS AIGLELWSMT SDIFFDNFII CADRRIVDDW
460 470 480 490 500
ANDGWGLKKA ADGAAEPGVV GQMIEAAEER PWLWVVYILT VALPVFLVIL
510 520 530 540 550
FCCSGKKQTS AMEYKKTDAP QPDVKEEEEE KEEEKDKGDE EEEGEEKLEE
560 570 580 590
KQKSDAEEDG GTVSQEEEDR KPKAEEDEIL NRSPRNRKPR RE
Length:592
Mass (Da):67,572
Last modified:July 24, 2006 - v2
Checksum:i31875C853C202811
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti83 – 831W → R in CAH93476 (Ref. 1) Curated
Sequence conflicti143 – 1431T → A in CAH92563 (Ref. 1) Curated
Sequence conflicti217 – 2171K → R in CAH92697 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR860439 mRNA. Translation: CAH92563.1.
CR860572 mRNA. Translation: CAH92697.1.
CR861420 mRNA. Translation: CAH93476.1.
RefSeqiNP_001127039.1. NM_001133567.1.
UniGeneiPab.359.

Genome annotation databases

GeneIDi100174065.
KEGGipon:100174065.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR860439 mRNA. Translation: CAH92563.1.
CR860572 mRNA. Translation: CAH92697.1.
CR861420 mRNA. Translation: CAH93476.1.
RefSeqiNP_001127039.1. NM_001133567.1.
UniGeneiPab.359.

3D structure databases

ProteinModelPortaliQ5R440.
SMRiQ5R440. Positions 60-457.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ5R440.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100174065.
KEGGipon:100174065.

Organism-specific databases

CTDi821.

Phylogenomic databases

HOVERGENiHBG005407.
InParanoidiQ5R440.
KOiK08054.

Family and domain databases

Gene3Di2.10.250.10. 1 hit.
2.60.120.200. 1 hit.
InterProiIPR001580. Calret/calnex.
IPR018124. Calret/calnex_CS.
IPR009033. Calreticulin/calnexin_P_dom.
IPR013320. ConA-like_dom.
[Graphical view]
PANTHERiPTHR11073. PTHR11073. 1 hit.
PfamiPF00262. Calreticulin. 1 hit.
[Graphical view]
PRINTSiPR00626. CALRETICULIN.
SUPFAMiSSF49899. SSF49899. 2 hits.
SSF63887. SSF63887. 1 hit.
PROSITEiPS00803. CALRETICULIN_1. 1 hit.
PS00804. CALRETICULIN_2. 1 hit.
PS00805. CALRETICULIN_REPEAT. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. The German cDNA consortium
    Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain cortex.

Entry informationi

Entry nameiCALX_PONAB
AccessioniPrimary (citable) accession number: Q5R440
Secondary accession number(s): Q5R6B9, Q5R6P7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 11, 2005
Last sequence update: July 24, 2006
Last modified: March 3, 2015
This is version 75 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.