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Q5R440

- CALX_PONAB

UniProt

Q5R440 - CALX_PONAB

Protein

Calnexin

Gene

CANX

Organism
Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 2 (25 Jul 2006)
      Previous versions | rss
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    Functioni

    Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi74 – 741Calcium; via carbonyl oxygenBy similarity
    Metal bindingi117 – 1171Calcium; via carbonyl oxygenBy similarity
    Binding sitei164 – 1641CarbohydrateBy similarity
    Binding sitei166 – 1661CarbohydrateBy similarity
    Binding sitei185 – 1851CarbohydrateBy similarity
    Binding sitei216 – 2161CarbohydrateBy similarity
    Metal bindingi436 – 4361CalciumBy similarity

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro

    GO - Biological processi

    1. clathrin-mediated endocytosis Source: UniProtKB
    2. protein folding Source: InterPro
    3. synaptic vesicle endocytosis Source: UniProtKB

    Keywords - Molecular functioni

    Chaperone

    Keywords - Ligandi

    Calcium, Lectin, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Calnexin
    Gene namesi
    Name:CANX
    OrganismiPongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
    Taxonomic identifieri9601 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaePongo
    ProteomesiUP000001595: Unplaced

    Subcellular locationi

    Endoplasmic reticulum membrane; Single-pass type I membrane protein. Endoplasmic reticulum By similarity. Melanosome By similarity
    Note: The palmitoylated form preferentially localizes to the perinuclear rough ER.By similarity

    GO - Cellular componenti

    1. endoplasmic reticulum Source: UniProtKB
    2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    3. integral component of membrane Source: UniProtKB-KW
    4. melanosome Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2020Sequence AnalysisAdd
    BLAST
    Chaini21 – 592572CalnexinPRO_0000004200Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei137 – 1371N6-acetyllysineBy similarity
    Disulfide bondi160 ↔ 194By similarity
    Disulfide bondi360 ↔ 366By similarity
    Lipidationi502 – 5021S-palmitoyl cysteineBy similarity
    Lipidationi503 – 5031S-palmitoyl cysteineBy similarity
    Modified residuei554 – 5541PhosphoserineBy similarity
    Modified residuei562 – 5621PhosphothreonineBy similarity
    Modified residuei564 – 5641Phosphoserine; by MAPK3By similarity
    Modified residuei583 – 5831PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylated at Ser-564 by MAPK3/ERK1. phosphorylation by MAPK3/ERK1 increases its association with ribosomes By similarity.By similarity
    Palmitoylation by DHHC6 leads to the preferential localization to the perinuclear rough ER. It mediates the association of calnexin with the ribosome-translocon complex (RTC) which is required for efficient folding of glycosylated proteins By similarity.By similarity

    Keywords - PTMi

    Acetylation, Disulfide bond, Lipoprotein, Palmitate, Phosphoprotein

    Proteomic databases

    PRIDEiQ5R440.

    Interactioni

    Subunit structurei

    Interacts with MAPK3/ERK1. Interacts with KCNH2 By similarity. Associates with ribosomes. Interacts with SGIP1; involved in negative regulation of endocytosis. The palmitoylated form interacts with the ribosome-translocon complex component SSR1, promoting efficient folding of glycoproteins By similarity. Interacts with KCNH2 By similarity. Interacts with SERPINA2 and with the S and Z variants of SERPINA1 By similarity. Part of a complex which includes CANX, DERL1, DERL2, DDOST/OST48, RPN1, RPN2, SELK, STT3A, VCP AND VIMP. Interacts with PPIB By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ5R440.
    SMRiQ5R440. Positions 60-457.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini21 – 481461LumenalSequence AnalysisAdd
    BLAST
    Topological domaini503 – 59290CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei482 – 50221HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati278 – 290131-1Add
    BLAST
    Repeati295 – 307131-2Add
    BLAST
    Repeati314 – 326131-3Add
    BLAST
    Repeati333 – 345131-4Add
    BLAST
    Repeati348 – 358112-1Add
    BLAST
    Repeati367 – 377112-2Add
    BLAST
    Repeati381 – 391112-3Add
    BLAST
    Repeati395 – 405112-4Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni276 – 409134P domain (Extended arm)By similarityAdd
    BLAST
    Regioni278 – 345684 X approximate repeatsAdd
    BLAST
    Regioni326 – 35934Interaction with PPIBBy similarityAdd
    BLAST
    Regioni348 – 405584 X approximate repeatsAdd
    BLAST
    Regioni503 – 59290Sufficient to mediate interaction with SGIP1By similarityAdd
    BLAST

    Sequence similaritiesi

    Belongs to the calreticulin family.Curated

    Keywords - Domaini

    Repeat, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    HOVERGENiHBG005407.
    KOiK08054.

    Family and domain databases

    Gene3Di2.10.250.10. 1 hit.
    2.60.120.200. 1 hit.
    InterProiIPR001580. Calret/calnex.
    IPR018124. Calret/calnex_CS.
    IPR009033. Calreticulin/calnexin_P_dom.
    IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    [Graphical view]
    PANTHERiPTHR11073. PTHR11073. 1 hit.
    PfamiPF00262. Calreticulin. 1 hit.
    [Graphical view]
    PRINTSiPR00626. CALRETICULIN.
    SUPFAMiSSF49899. SSF49899. 2 hits.
    SSF63887. SSF63887. 1 hit.
    PROSITEiPS00803. CALRETICULIN_1. 1 hit.
    PS00804. CALRETICULIN_2. 1 hit.
    PS00805. CALRETICULIN_REPEAT. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q5R440-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEGKWLLCML LVLGTAIVEA HDGHDDDVID IEDDLDDVIE EVEDSKPDTT    50
    APPSSPKVTY KAPVPTGEVY FADSFDRGTL SGWILSKAKK DDTDDEIAKY 100
    DGKWEVDEMK ESKLPGDKGL VLMSRAKHHA ISAKLNKPFL FDTKPLIVQY 150
    EVNFQNGIEC GGAYVKLLSK TPELNLDQFH DKTPYTIMFG PDKCGEDYKL 200
    HFIFRHKNPK TGIYEEKHAK RPDADLKTYF TDKKTHLYTL ILNPDNSFEI 250
    LVDQSVVNSG NLLNDMTPPV NPSREIEDPE DRKPEDWDER PKIPDPEAVK 300
    PDDWDEDAPA KIPDEEATKP EGWLDDEPEY VPDPDAEKPE DWDEDMDGEW 350
    EAPQIANPKC ESAPGCGVWQ RPMIDNPNYK GKWKPPMIDN PSYQGIWKPR 400
    KIPNPDFFED LEPFRMTPFS AIGLELWSMT SDIFFDNFII CADRRIVDDW 450
    ANDGWGLKKA ADGAAEPGVV GQMIEAAEER PWLWVVYILT VALPVFLVIL 500
    FCCSGKKQTS AMEYKKTDAP QPDVKEEEEE KEEEKDKGDE EEEGEEKLEE 550
    KQKSDAEEDG GTVSQEEEDR KPKAEEDEIL NRSPRNRKPR RE 592
    Length:592
    Mass (Da):67,572
    Last modified:July 25, 2006 - v2
    Checksum:i31875C853C202811
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti83 – 831W → R in CAH93476. 1 PublicationCurated
    Sequence conflicti143 – 1431T → A in CAH92563. 1 PublicationCurated
    Sequence conflicti217 – 2171K → R in CAH92697. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR860439 mRNA. Translation: CAH92563.1.
    CR860572 mRNA. Translation: CAH92697.1.
    CR861420 mRNA. Translation: CAH93476.1.
    RefSeqiNP_001127039.1. NM_001133567.1.
    UniGeneiPab.359.

    Genome annotation databases

    GeneIDi100174065.
    KEGGipon:100174065.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR860439 mRNA. Translation: CAH92563.1 .
    CR860572 mRNA. Translation: CAH92697.1 .
    CR861420 mRNA. Translation: CAH93476.1 .
    RefSeqi NP_001127039.1. NM_001133567.1.
    UniGenei Pab.359.

    3D structure databases

    ProteinModelPortali Q5R440.
    SMRi Q5R440. Positions 60-457.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q5R440.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100174065.
    KEGGi pon:100174065.

    Organism-specific databases

    CTDi 821.

    Phylogenomic databases

    HOVERGENi HBG005407.
    KOi K08054.

    Family and domain databases

    Gene3Di 2.10.250.10. 1 hit.
    2.60.120.200. 1 hit.
    InterProi IPR001580. Calret/calnex.
    IPR018124. Calret/calnex_CS.
    IPR009033. Calreticulin/calnexin_P_dom.
    IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    [Graphical view ]
    PANTHERi PTHR11073. PTHR11073. 1 hit.
    Pfami PF00262. Calreticulin. 1 hit.
    [Graphical view ]
    PRINTSi PR00626. CALRETICULIN.
    SUPFAMi SSF49899. SSF49899. 2 hits.
    SSF63887. SSF63887. 1 hit.
    PROSITEi PS00803. CALRETICULIN_1. 1 hit.
    PS00804. CALRETICULIN_2. 1 hit.
    PS00805. CALRETICULIN_REPEAT. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. The German cDNA consortium
      Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain cortex.

    Entry informationi

    Entry nameiCALX_PONAB
    AccessioniPrimary (citable) accession number: Q5R440
    Secondary accession number(s): Q5R6B9, Q5R6P7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 12, 2005
    Last sequence update: July 25, 2006
    Last modified: October 1, 2014
    This is version 72 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3