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Q5R440

- CALX_PONAB

UniProt

Q5R440 - CALX_PONAB

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Protein

Calnexin

Gene
CANX
Organism
Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi74 – 741Calcium; via carbonyl oxygen By similarity
Metal bindingi117 – 1171Calcium; via carbonyl oxygen By similarity
Binding sitei164 – 1641Carbohydrate By similarity
Binding sitei166 – 1661Carbohydrate By similarity
Binding sitei185 – 1851Carbohydrate By similarity
Binding sitei216 – 2161Carbohydrate By similarity
Metal bindingi436 – 4361Calcium By similarity

GO - Molecular functioni

  1. calcium ion binding Source: InterPro

GO - Biological processi

  1. clathrin-mediated endocytosis Source: UniProtKB
  2. protein folding Source: InterPro
  3. synaptic vesicle endocytosis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Ligandi

Calcium, Lectin, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Calnexin
Gene namesi
Name:CANX
OrganismiPongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Taxonomic identifieri9601 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaePongo
ProteomesiUP000001595: Unplaced

Subcellular locationi

Endoplasmic reticulum membrane; Single-pass type I membrane protein. Endoplasmic reticulum By similarity. Melanosome By similarity
Note: The palmitoylated form preferentially localizes to the perinuclear rough ER By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini21 – 481461Lumenal Reviewed predictionAdd
BLAST
Transmembranei482 – 50221Helical; Reviewed predictionAdd
BLAST
Topological domaini503 – 59290Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB
  2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  3. integral component of membrane Source: UniProtKB-KW
  4. melanosome Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020 Reviewed predictionAdd
BLAST
Chaini21 – 592572CalnexinPRO_0000004200Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei137 – 1371N6-acetyllysine By similarity
Disulfide bondi160 ↔ 194 By similarity
Disulfide bondi360 ↔ 366 By similarity
Lipidationi502 – 5021S-palmitoyl cysteine By similarity
Lipidationi503 – 5031S-palmitoyl cysteine By similarity
Modified residuei554 – 5541Phosphoserine By similarity
Modified residuei562 – 5621Phosphothreonine By similarity
Modified residuei564 – 5641Phosphoserine; by MAPK3 By similarity
Modified residuei583 – 5831Phosphoserine By similarity

Post-translational modificationi

Phosphorylated at Ser-564 by MAPK3/ERK1. phosphorylation by MAPK3/ERK1 increases its association with ribosomes By similarity.
Palmitoylation by DHHC6 leads to the preferential localization to the perinuclear rough ER. It mediates the association of calnexin with the ribosome-translocon complex (RTC) which is required for efficient folding of glycosylated proteins By similarity.

Keywords - PTMi

Acetylation, Disulfide bond, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PRIDEiQ5R440.

Interactioni

Subunit structurei

Interacts with MAPK3/ERK1. Interacts with KCNH2 By similarity. Associates with ribosomes. Interacts with SGIP1; involved in negative regulation of endocytosis. The palmitoylated form interacts with the ribosome-translocon complex component SSR1, promoting efficient folding of glycoproteins By similarity. Interacts with KCNH2 By similarity. Interacts with SERPINA2 and with the S and Z variants of SERPINA1 By similarity. Part of a complex which includes CANX, DERL1, DERL2, DDOST/OST48, RPN1, RPN2, SELK, STT3A, VCP AND VIMP. Interacts with PPIB By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ5R440.
SMRiQ5R440. Positions 60-457.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati278 – 290131-1Add
BLAST
Repeati295 – 307131-2Add
BLAST
Repeati314 – 326131-3Add
BLAST
Repeati333 – 345131-4Add
BLAST
Repeati348 – 358112-1Add
BLAST
Repeati367 – 377112-2Add
BLAST
Repeati381 – 391112-3Add
BLAST
Repeati395 – 405112-4Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni276 – 409134P domain (Extended arm) By similarityAdd
BLAST
Regioni278 – 345684 X approximate repeatsAdd
BLAST
Regioni326 – 35934Interaction with PPIB By similarityAdd
BLAST
Regioni348 – 405584 X approximate repeatsAdd
BLAST
Regioni503 – 59290Sufficient to mediate interaction with SGIP1 By similarityAdd
BLAST

Sequence similaritiesi

Belongs to the calreticulin family.

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG005407.
KOiK08054.

Family and domain databases

Gene3Di2.10.250.10. 1 hit.
2.60.120.200. 1 hit.
InterProiIPR001580. Calret/calnex.
IPR018124. Calret/calnex_CS.
IPR009033. Calreticulin/calnexin_P_dom.
IPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
[Graphical view]
PANTHERiPTHR11073. PTHR11073. 1 hit.
PfamiPF00262. Calreticulin. 1 hit.
[Graphical view]
PRINTSiPR00626. CALRETICULIN.
SUPFAMiSSF49899. SSF49899. 2 hits.
SSF63887. SSF63887. 1 hit.
PROSITEiPS00803. CALRETICULIN_1. 1 hit.
PS00804. CALRETICULIN_2. 1 hit.
PS00805. CALRETICULIN_REPEAT. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5R440-1 [UniParc]FASTAAdd to Basket

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MEGKWLLCML LVLGTAIVEA HDGHDDDVID IEDDLDDVIE EVEDSKPDTT    50
APPSSPKVTY KAPVPTGEVY FADSFDRGTL SGWILSKAKK DDTDDEIAKY 100
DGKWEVDEMK ESKLPGDKGL VLMSRAKHHA ISAKLNKPFL FDTKPLIVQY 150
EVNFQNGIEC GGAYVKLLSK TPELNLDQFH DKTPYTIMFG PDKCGEDYKL 200
HFIFRHKNPK TGIYEEKHAK RPDADLKTYF TDKKTHLYTL ILNPDNSFEI 250
LVDQSVVNSG NLLNDMTPPV NPSREIEDPE DRKPEDWDER PKIPDPEAVK 300
PDDWDEDAPA KIPDEEATKP EGWLDDEPEY VPDPDAEKPE DWDEDMDGEW 350
EAPQIANPKC ESAPGCGVWQ RPMIDNPNYK GKWKPPMIDN PSYQGIWKPR 400
KIPNPDFFED LEPFRMTPFS AIGLELWSMT SDIFFDNFII CADRRIVDDW 450
ANDGWGLKKA ADGAAEPGVV GQMIEAAEER PWLWVVYILT VALPVFLVIL 500
FCCSGKKQTS AMEYKKTDAP QPDVKEEEEE KEEEKDKGDE EEEGEEKLEE 550
KQKSDAEEDG GTVSQEEEDR KPKAEEDEIL NRSPRNRKPR RE 592
Length:592
Mass (Da):67,572
Last modified:July 25, 2006 - v2
Checksum:i31875C853C202811
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti83 – 831W → R in CAH93476. 1 Publication
Sequence conflicti143 – 1431T → A in CAH92563. 1 Publication
Sequence conflicti217 – 2171K → R in CAH92697. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR860439 mRNA. Translation: CAH92563.1.
CR860572 mRNA. Translation: CAH92697.1.
CR861420 mRNA. Translation: CAH93476.1.
RefSeqiNP_001127039.1. NM_001133567.1.
UniGeneiPab.359.

Genome annotation databases

GeneIDi100174065.
KEGGipon:100174065.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR860439 mRNA. Translation: CAH92563.1 .
CR860572 mRNA. Translation: CAH92697.1 .
CR861420 mRNA. Translation: CAH93476.1 .
RefSeqi NP_001127039.1. NM_001133567.1.
UniGenei Pab.359.

3D structure databases

ProteinModelPortali Q5R440.
SMRi Q5R440. Positions 60-457.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q5R440.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 100174065.
KEGGi pon:100174065.

Organism-specific databases

CTDi 821.

Phylogenomic databases

HOVERGENi HBG005407.
KOi K08054.

Family and domain databases

Gene3Di 2.10.250.10. 1 hit.
2.60.120.200. 1 hit.
InterProi IPR001580. Calret/calnex.
IPR018124. Calret/calnex_CS.
IPR009033. Calreticulin/calnexin_P_dom.
IPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
[Graphical view ]
PANTHERi PTHR11073. PTHR11073. 1 hit.
Pfami PF00262. Calreticulin. 1 hit.
[Graphical view ]
PRINTSi PR00626. CALRETICULIN.
SUPFAMi SSF49899. SSF49899. 2 hits.
SSF63887. SSF63887. 1 hit.
PROSITEi PS00803. CALRETICULIN_1. 1 hit.
PS00804. CALRETICULIN_2. 1 hit.
PS00805. CALRETICULIN_REPEAT. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. The German cDNA consortium
    Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain cortex.

Entry informationi

Entry nameiCALX_PONAB
AccessioniPrimary (citable) accession number: Q5R440
Secondary accession number(s): Q5R6B9, Q5R6P7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: July 25, 2006
Last modified: April 16, 2014
This is version 71 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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