Q5R411 (PACN1_PONAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein kinase C and casein kinase substrate in neurons protein 1

Gene names

Name:

Pacsin1

Organism

Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii) [Reference proteome]

Taxonomic identifier

9601 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Ponginae › Pongo

Protein attributes

Sequence length	444 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May play a role in endocytosis By similarity.
Subunit structure	May form homo- and heterooligomers with other PACSINs. Interacts with DNM1, SYNJ1 and WASL through its SH3 domain By similarity.
Subcellular location	Cytoplasm By similarity. Cell projection By similarity. Note: In primary neuronal cultures, present at a high level in presynaptic nerve terminals and in the cell body. Colocalizes with DNM1 at vesicular structures in the cell body and neurites By similarity.
Post-translational modification	Phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC) Probable.
Sequence similarities	Belongs to the PACSIN family. Contains 1 FCH domain. Contains 1 SH3 domain.

Ontologies

Keywords
Biological process	Endocytosis
Cellular component	Cell projection Cytoplasm
Domain	Coiled coil SH3 domain
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	endocytosis Inferred from electronic annotation. Source: UniProtKB-KW membrane tubulation Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of endocytosis Inferred from electronic annotation. Source: Compara neuron projection morphogenesis Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	COPI-coated vesicle Inferred from electronic annotation. Source: Compara axon terminus Inferred from sequence or structural similarity. Source: UniProtKB cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB ruffle membrane Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	phospholipid binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 444	444	Protein kinase C and casein kinase substrate in neurons protein 1		PRO_0000351650
Regions
	Domain	13 – 76	64	FCH
	Domain	385 – 444	60	SH3
	Coiled coil	148 – 242	95	Potential
Amino acid modifications
	Modified residue	394	1	Phosphotyrosine By similarity
Experimental info
	Sequence conflict	113	1	V → A in CAH91500. Ref.1
	Sequence conflict	141	1	K → R in CAH91500. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q5R411 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: 2BBE3A6E7374CE49	FASTA	444	50,922
	10 20 30 40 50 60 MSSSYDEASL APEETTDSFW EVGNYKRTVK RIDDGHRLCN DLMNCVQERA KIEKAYGQQL 70 80 90 100 110 120 TDWAKRWRQL IEKGPQYGSL ERAWGAIMTE ADKVSELHQE VKNNLLNEDL EKVKNWQKDA 130 140 150 160 170 180 YHKQIMGGFK ETKEAEDGFR KAQKPWAKKM KELEAAKKAY HLACKEEKLA MTREMNSKSE 190 200 210 220 230 240 QSVTPEQQKK LQDKVDKCKQ DVQKTQEKYE KVLEDVGKTT PQYMENMEQV FEQCQQFEEK 250 260 270 280 290 300 RLVFLKEVLL DIKRHLNLAE NSSYIHVYRE LEQAIRGADA QEDLRWFRST SGPGMPMNWP 310 320 330 340 350 360 QFEEWNPDLP HTTTKKEKQP KKAEGVALTN ATGAVESTSQ AGDRGSVSSY DRGQPYATEW 370 380 390 400 410 420 SDDESGNPFG GSEANGGANP FEDDSKGVRV RALYDYDGQE QDELSFKAGD ELTKLGEEDE 430 440 QGWCRGRLDS GQLGLYPANY VEAI

References

[1]	The German cDNA consortium Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain cortex.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR859322 mRNA. Translation: CAH91500.1. CR861449 mRNA. Translation: CAH93505.1.
RefSeq	NP_001125882.1. NM_001132410.1.
UniGene	Pab.18449.
3D structure databases
HSSP	HSSP built from PDB template 2CSI based on UniProtKB O15034.
ProteinModelPortal	Q5R411.
ModBase	Search...
Proteomic databases
PRIDE	Q5R411.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSPPYT00000019207; ENSPPYP00000018470; ENSPPYG00000016517.
GeneID	100172814.
KEGG	pon:100172814.
Organism-specific databases
CTD	29993.
Phylogenomic databases
GeneTree	ENSGT00510000046376.
HOGENOM	HOG000007245.
HOVERGEN	HBG053486.
InParanoid	Q5R411.
OMA	PDSLGWC.
OrthoDB	EOG4QVCC5.
Family and domain databases
InterPro	IPR001060. FCH_dom. IPR001452. SH3_domain. [Graphical view]
Pfam	PF00611. FCH. 1 hit. PF00018. SH3_1. 1 hit. [Graphical view]
PRINTS	PR00452. SH3DOMAIN.
SMART	SM00055. FCH. 1 hit. SM00326. SH3. 1 hit. [Graphical view]
SUPFAM	SSF50044. SH3. 1 hit.
PROSITE	PS50133. FCH. 1 hit. PS50002. SH3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PACN1_PONAB

Accession

Primary (citable) accession number: Q5R411
Secondary accession number(s): Q5R9R0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 14, 2008
Last sequence update:	December 21, 2004
Last modified:	May 1, 2013
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program