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Protein

Protein kinase C and casein kinase substrate in neurons protein 1

Gene

Pacsin1

Organism
Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Binds to membranes via its F-BAR domain and mediates membrane tubulation. Plays a role in the reorganization of the microtubule cytoskeleton via its interaction with MAPT; this decreases microtubule stability and inhibits MAPT-induced microtubule polymerization. Plays a role in cellular transport processes by recruiting DNM1, DNM2 and DNM3 to membranes. Plays a role in the reorganization of the actin cytoskeleton and in neuron morphogenesis via its interaction with COBL and WASL, and by recruiting COBL to the cell cortex. Plays a role in the regulation of neurite formation, neurite branching and the regulation of neurite length. Required for normal synaptic vesicle endocytosis; this process retrieves previously released neurotransmitters to accommodate multiple cycles of neurotransmission. Required for normal excitatory and inhibitory synaptic transmission (By similarity).By similarity

GO - Molecular functioni

  1. phospholipid binding Source: UniProtKB

GO - Biological processi

  1. actin filament organization Source: InterPro
  2. membrane tubulation Source: UniProtKB
  3. negative regulation of endocytosis Source: Ensembl
  4. neuron projection morphogenesis Source: UniProtKB
  5. protein localization to membrane Source: UniProtKB
  6. synaptic vesicle endocytosis Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Endocytosis

Keywords - Ligandi

Lipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein kinase C and casein kinase substrate in neurons protein 1
Gene namesi
Name:Pacsin1
OrganismiPongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Taxonomic identifieri9601 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaePongo
ProteomesiUP000001595: Chromosome 6

Subcellular locationi

Cytoplasm By similarity. Cell projection By similarity. Cell junctionsynapsesynaptosome By similarity. Cell projectionruffle membrane By similarity. Membrane By similarity; Peripheral membrane protein By similarity. Cytoplasmic vesicle membrane By similarity; Peripheral membrane protein By similarity. Cell junctionsynapse By similarity. Cytoplasmcytosol. Cell membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
Note: In primary neuronal cultures, present at a high level in presynaptic nerve terminals and in the cell body. Colocalizes with DNM1 at vesicular structures in the cell body and neurites. Colocalizes with MAPT in axons (By similarity).By similarity

GO - Cellular componenti

  1. axon terminus Source: UniProtKB
  2. cell junction Source: UniProtKB-KW
  3. COPI-coated vesicle Source: Ensembl
  4. cytoplasm Source: UniProtKB
  5. cytoplasmic vesicle membrane Source: UniProtKB-SubCell
  6. cytosol Source: UniProtKB-SubCell
  7. ruffle membrane Source: UniProtKB
  8. synapse Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Membrane, Synapse, Synaptosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 444444Protein kinase C and casein kinase substrate in neurons protein 1PRO_0000351650Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei79 – 791PhosphoserineBy similarity
Modified residuei184 – 1841PhosphothreonineBy similarity
Modified residuei394 – 3941PhosphotyrosineBy similarity

Post-translational modificationi

Phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC).Curated

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ5R411.

Interactioni

Subunit structurei

Homodimer. May form heterooligomers with other PACSINs. Interacts with both COBL and DBNL. Identified in a complex composed of COBL, PACSIN1 and WASL. Interacts (via SH3 domain) with SYNJ1 and WASL. Interacts (via SH3 domain) with DNM1; the interaction is reduced by DNM1 phosphorylation. Interacts with DNM2 and DNM3. Interacts with MAPT. Interacts with EHD1 and EHD3. Interacts with TRPV4 (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ5R411.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini13 – 7664FCHPROSITE-ProRule annotationAdd
BLAST
Domaini385 – 44460SH3PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 307307F-BAR domainBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili26 – 275250By similarityAdd
BLAST

Sequence similaritiesi

Belongs to the PACSIN family.Curated
Contains 1 FCH domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, SH3 domain

Phylogenomic databases

GeneTreeiENSGT00510000046376.
HOGENOMiHOG000007245.
HOVERGENiHBG053486.
InParanoidiQ5R411.
OMAiAMTREAN.
OrthoDBiEOG75TMBJ.
TreeFamiTF313677.

Family and domain databases

InterProiIPR001060. FCH_dom.
IPR028518. PACSIN1.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR10959:SF7. PTHR10959:SF7. 1 hit.
PfamiPF00611. FCH. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00055. FCH. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS50133. FCH. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5R411-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSSSYDEASL APEETTDSFW EVGNYKRTVK RIDDGHRLCN DLMNCVQERA
60 70 80 90 100
KIEKAYGQQL TDWAKRWRQL IEKGPQYGSL ERAWGAIMTE ADKVSELHQE
110 120 130 140 150
VKNNLLNEDL EKVKNWQKDA YHKQIMGGFK ETKEAEDGFR KAQKPWAKKM
160 170 180 190 200
KELEAAKKAY HLACKEEKLA MTREMNSKSE QSVTPEQQKK LQDKVDKCKQ
210 220 230 240 250
DVQKTQEKYE KVLEDVGKTT PQYMENMEQV FEQCQQFEEK RLVFLKEVLL
260 270 280 290 300
DIKRHLNLAE NSSYIHVYRE LEQAIRGADA QEDLRWFRST SGPGMPMNWP
310 320 330 340 350
QFEEWNPDLP HTTTKKEKQP KKAEGVALTN ATGAVESTSQ AGDRGSVSSY
360 370 380 390 400
DRGQPYATEW SDDESGNPFG GSEANGGANP FEDDSKGVRV RALYDYDGQE
410 420 430 440
QDELSFKAGD ELTKLGEEDE QGWCRGRLDS GQLGLYPANY VEAI
Length:444
Mass (Da):50,922
Last modified:December 21, 2004 - v1
Checksum:i2BBE3A6E7374CE49
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti113 – 1131V → A in CAH91500. 1 PublicationCurated
Sequence conflicti141 – 1411K → R in CAH91500. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR859322 mRNA. Translation: CAH91500.1.
CR861449 mRNA. Translation: CAH93505.1.
RefSeqiNP_001125882.1. NM_001132410.1.
XP_009240032.1. XM_009241757.1.

Genome annotation databases

EnsembliENSPPYT00000019207; ENSPPYP00000018470; ENSPPYG00000016517.
GeneIDi100172814.
KEGGipon:100172814.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR859322 mRNA. Translation: CAH91500.1.
CR861449 mRNA. Translation: CAH93505.1.
RefSeqiNP_001125882.1. NM_001132410.1.
XP_009240032.1. XM_009241757.1.

3D structure databases

ProteinModelPortaliQ5R411.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ5R411.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSPPYT00000019207; ENSPPYP00000018470; ENSPPYG00000016517.
GeneIDi100172814.
KEGGipon:100172814.

Organism-specific databases

CTDi29993.

Phylogenomic databases

GeneTreeiENSGT00510000046376.
HOGENOMiHOG000007245.
HOVERGENiHBG053486.
InParanoidiQ5R411.
OMAiAMTREAN.
OrthoDBiEOG75TMBJ.
TreeFamiTF313677.

Family and domain databases

InterProiIPR001060. FCH_dom.
IPR028518. PACSIN1.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR10959:SF7. PTHR10959:SF7. 1 hit.
PfamiPF00611. FCH. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00055. FCH. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS50133. FCH. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. The German cDNA consortium
    Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain cortex.

Entry informationi

Entry nameiPACN1_PONAB
AccessioniPrimary (citable) accession number: Q5R411
Secondary accession number(s): Q5R9R0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 14, 2008
Last sequence update: December 21, 2004
Last modified: January 7, 2015
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.