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Q5R411 (PACN1_PONAB) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein kinase C and casein kinase substrate in neurons protein 1
Gene names
Name:Pacsin1
OrganismPongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii) [Reference proteome]
Taxonomic identifier9601 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaePongo

Protein attributes

Sequence length444 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Binds to membranes via its F-BAR domain and mediates membrane tubulation. Plays a role in the reorganization of the microtubule cytoskeleton via its interaction with MAPT; this decreases microtubule stability and inhibits MAPT-induced microtubule polymerization. Plays a role in cellular transport processes by recruiting DNM1, DNM2 and DNM3 to membranes. Plays a role in the reorganization of the actin cytoskeleton and in neuron morphogenesis via its interaction with COBL and WASL, and by recruiting COBL to the cell cortex. Plays a role in the regulation of neurite formation, neurite branching and the regulation of neurite length. Required for normal synaptic vesicle endocytosis; this process retrieves previously released neurotransmitters to accommodate multiple cycles of neurotransmission. Required for normal excitatory and inhibitory synaptic transmission By similarity.

Subunit structure

Homodimer. May form heterooligomers with other PACSINs. Interacts with both COBL and DBNL. Identified in a complex composed of COBL, PACSIN1 and WASL. Interacts (via SH3 domain) with SYNJ1 and WASL. Interacts (via SH3 domain) with DNM1; the interaction is reduced by DNM1 phosphorylation. Interacts with DNM2 and DNM3. Interacts with MAPT. Interacts with EHD1 and EHD3. Interacts with TRPV4 By similarity.

Subcellular location

Cytoplasm By similarity. Cell projection By similarity. Cell junctionsynapsesynaptosome By similarity. Cell projectionruffle membrane By similarity. Membrane; Peripheral membrane protein By similarity. Cytoplasmic vesicle membrane; Peripheral membrane protein By similarity. Cell junctionsynapse By similarity. Cytoplasmcytosol. Cell membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Note: In primary neuronal cultures, present at a high level in presynaptic nerve terminals and in the cell body. Colocalizes with DNM1 at vesicular structures in the cell body and neurites. Colocalizes with MAPT in axons By similarity.

Post-translational modification

Phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC) Probable.

Sequence similarities

Belongs to the PACSIN family.

Contains 1 FCH domain.

Contains 1 SH3 domain.

Ontologies

Keywords
   Biological processEndocytosis
   Cellular componentCell junction
Cell membrane
Cell projection
Cytoplasm
Cytoplasmic vesicle
Membrane
Synapse
Synaptosome
   DomainCoiled coil
SH3 domain
   LigandLipid-binding
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processactin filament organization

Inferred from electronic annotation. Source: InterPro

membrane tubulation

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of endocytosis

Inferred from electronic annotation. Source: Ensembl

neuron projection morphogenesis

Inferred from sequence or structural similarity. Source: UniProtKB

protein localization to membrane

Inferred from sequence or structural similarity. Source: UniProtKB

synaptic vesicle endocytosis

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular_componentCOPI-coated vesicle

Inferred from electronic annotation. Source: Ensembl

axon terminus

Inferred from sequence or structural similarity. Source: UniProtKB

cell junction

Inferred from electronic annotation. Source: UniProtKB-KW

cytoplasm

Inferred from sequence or structural similarity. Source: UniProtKB

cytoplasmic vesicle membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

cytosol

Inferred from electronic annotation. Source: UniProtKB-SubCell

ruffle membrane

Inferred from sequence or structural similarity. Source: UniProtKB

synapse

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionphospholipid binding

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 444444Protein kinase C and casein kinase substrate in neurons protein 1
PRO_0000351650

Regions

Domain13 – 7664FCH
Domain385 – 44460SH3
Region1 – 307307F-BAR domain By similarity
Coiled coil26 – 275250 By similarity

Amino acid modifications

Modified residue791Phosphoserine By similarity
Modified residue1841Phosphothreonine By similarity
Modified residue3941Phosphotyrosine By similarity

Experimental info

Sequence conflict1131V → A in CAH91500. Ref.1
Sequence conflict1411K → R in CAH91500. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q5R411 [UniParc].

Last modified December 21, 2004. Version 1.
Checksum: 2BBE3A6E7374CE49

FASTA44450,922
        10         20         30         40         50         60 
MSSSYDEASL APEETTDSFW EVGNYKRTVK RIDDGHRLCN DLMNCVQERA KIEKAYGQQL 

        70         80         90        100        110        120 
TDWAKRWRQL IEKGPQYGSL ERAWGAIMTE ADKVSELHQE VKNNLLNEDL EKVKNWQKDA 

       130        140        150        160        170        180 
YHKQIMGGFK ETKEAEDGFR KAQKPWAKKM KELEAAKKAY HLACKEEKLA MTREMNSKSE 

       190        200        210        220        230        240 
QSVTPEQQKK LQDKVDKCKQ DVQKTQEKYE KVLEDVGKTT PQYMENMEQV FEQCQQFEEK 

       250        260        270        280        290        300 
RLVFLKEVLL DIKRHLNLAE NSSYIHVYRE LEQAIRGADA QEDLRWFRST SGPGMPMNWP 

       310        320        330        340        350        360 
QFEEWNPDLP HTTTKKEKQP KKAEGVALTN ATGAVESTSQ AGDRGSVSSY DRGQPYATEW 

       370        380        390        400        410        420 
SDDESGNPFG GSEANGGANP FEDDSKGVRV RALYDYDGQE QDELSFKAGD ELTKLGEEDE 

       430        440 
QGWCRGRLDS GQLGLYPANY VEAI 

« Hide

References

[1]The German cDNA consortium
Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain cortex.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CR859322 mRNA. Translation: CAH91500.1.
CR861449 mRNA. Translation: CAH93505.1.
RefSeqNP_001125882.1. NM_001132410.1.

3D structure databases

ProteinModelPortalQ5R411.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ5R411.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSPPYT00000019207; ENSPPYP00000018470; ENSPPYG00000016517.
GeneID100172814.
KEGGpon:100172814.

Organism-specific databases

CTD29993.

Phylogenomic databases

GeneTreeENSGT00510000046376.
HOGENOMHOG000007245.
HOVERGENHBG053486.
InParanoidQ5R411.
OMAAMTREAN.
OrthoDBEOG75TMBJ.
TreeFamTF313677.

Family and domain databases

InterProIPR001060. FCH_dom.
IPR028518. PACSIN1.
IPR001452. SH3_domain.
[Graphical view]
PANTHERPTHR10959:SF0. PTHR10959:SF0. 1 hit.
PfamPF00611. FCH. 1 hit.
[Graphical view]
PRINTSPR00452. SH3DOMAIN.
SMARTSM00055. FCH. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMSSF50044. SSF50044. 1 hit.
PROSITEPS50133. FCH. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePACN1_PONAB
AccessionPrimary (citable) accession number: Q5R411
Secondary accession number(s): Q5R9R0
Entry history
Integrated into UniProtKB/Swiss-Prot: October 14, 2008
Last sequence update: December 21, 2004
Last modified: February 19, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families