Q5R1M5 (CELA1_FELCA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chymotrypsin-like elastase family member 1
EC=3.4.21.36

Alternative name(s):
Elastase-1

Gene names

Name:	CELA1
Synonyms:	ELA1, ELS1

Organism

Felis catus (Cat) (Felis silvestris catus) [Complete proteome]

Taxonomic identifier

9685 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Carnivora › Feliformia › Felidae › Felinae › Felis

Protein attributes

Sequence length	266 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Acts upon elastin By similarity.
Catalytic activity	Hydrolysis of proteins, including elastin. Preferential cleavage: Ala-|-Xaa.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Subcellular location	Secreted.
Sequence similarities	Belongs to the peptidase S1 family. Elastase subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	Wnt receptor signaling pathway Inferred from electronic annotation. Source: Compara exocrine pancreas development Inferred from electronic annotation. Source: Compara inflammatory response Inferred from electronic annotation. Source: Compara multicellular organism growth Inferred from electronic annotation. Source: Compara negative regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara pancreas morphogenesis Inferred from electronic annotation. Source: Compara positive regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara post-embryonic development Inferred from electronic annotation. Source: Compara proteolysis Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell differentiation Inferred from electronic annotation. Source: Compara regulation of cell proliferation Inferred from electronic annotation. Source: Compara
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Potential
	Propeptide	17 – 26	10	Activation peptide		PRO_0000027675
	Chain	27 – 266	240	Chymotrypsin-like elastase family member 1		PRO_0000027676
Regions
	Domain	27 – 264	238	Peptidase S1
Sites
	Active site	71	1	Charge relay system By similarity
	Active site	119	1	Charge relay system By similarity
	Active site	214	1	Charge relay system By similarity
	Metal binding	85	1	Calcium By similarity
	Metal binding	90	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	95	1	Calcium By similarity
Amino acid modifications
	Glycosylation	87	1	N-linked (GlcNAc...) Potential
	Glycosylation	241	1	N-linked (GlcNAc...) Potential
	Disulfide bond	56 ↔ 72		By similarity
	Disulfide bond	153 ↔ 220		By similarity
	Disulfide bond	184 ↔ 200		By similarity
	Disulfide bond	210 ↔ 240		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5R1M5 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: 5CF022A502C03AB2	FASTA	266	28,841
	10 20 30 40 50 60 MLRFLVLATL VLYGHSTRDF PETNARVVGG TEARKNPWPS QISLQYLSGG KWYHTCGGTL 70 80 90 100 110 120 IRQNWVMTAA HCVDRKMTFR VVAGEHNLSQ NDGTEQRVSV QKIVVHPYWN SNNVAAGYDI 130 140 150 160 170 180 ALLRLAQRVT LNNYVQLGVL PAAGTILANN NPCYITGWGM TKTNGQLAQA LQQAYLPSVD 190 200 210 220 230 240 YATCSSSSYW GSTVKSTMVC AGGDGIRSGC QGDSGGPLHC LVNGKYAVHG VTSFVSSLGC 250 260 NVSRKPTVFT RVSAYISWIN NVIASN

References

[1]	"Felis catus ELS1 mRNA for elastase 1, complete cds." Sato N., Kano R., Hasegawa A. Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Pancreas.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB196342 mRNA. Translation: BAD77813.1.
RefSeq	NP_001009389.1. NM_001009389.2.
3D structure databases
ProteinModelPortal	Q5R1M5.
SMR	Q5R1M5. Positions 27-266.
ModBase	Search...
Protein family/group databases
MEROPS	S01.153.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSFCAT00000004412; ENSFCAP00000004073; ENSFCAG00000004411.
GeneID	494217.
KEGG	fca:494217.
Organism-specific databases
CTD	1990.
Phylogenomic databases
eggNOG	COG5640.
GeneTree	ENSGT00640000091262.
KO	K01326.
OrthoDB	EOG4V171P.
Family and domain databases
InterPro	IPR001254. Peptidase_S1. IPR018114. Peptidase_S1_AS. IPR001314. Peptidase_S1A. IPR009003. Trypsin-like_Pept_dom. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CELA1_FELCA

Accession

Primary (citable) accession number: Q5R1M5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	January 4, 2005
Last modified:	May 1, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program