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Q5QZS6

- SYI_IDILO

UniProt

Q5QZS6 - SYI_IDILO

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Idiomarina loihiensis (strain ATCC BAA-735 / DSM 15497 / L2-TR)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei568 – 5681Aminoacyl-adenylateUniRule annotation
Binding sitei612 – 6121ATPUniRule annotation
Metal bindingi907 – 9071ZincUniRule annotation
Metal bindingi910 – 9101ZincUniRule annotation
Metal bindingi927 – 9271ZincUniRule annotation
Metal bindingi930 – 9301ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciILOI283942:GI0U-1133-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:IL1128
OrganismiIdiomarina loihiensis (strain ATCC BAA-735 / DSM 15497 / L2-TR)
Taxonomic identifieri283942 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesIdiomarinaceaeIdiomarina
ProteomesiUP000001171: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 944944Isoleucine--tRNA ligasePRO_0000098400Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi283942.IL1128.

Structurei

3D structure databases

ProteinModelPortaliQ5QZS6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi609 – 6135"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5QZS6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSDYKHTLNL PETAFPMRGN LAQREPKMLE QWYETDLYGQ IRKAKAGKPK
60 70 80 90 100
FILHDGPPYA NGQIHIGHAV NKILKDVIVK AKTLSDFDAP YVPGWDCHGL
110 120 130 140 150
PIELQVEKKH GKPGKKLNLA EFREKCREYA MTQIDQQRTD FKRLGVLGDW
160 170 180 190 200
DNPYLTMNFK QEADSVRALA KIIDNGHMHQ GFKPVHWCTD CGSALAEAEV
210 220 230 240 250
EYQDKNSPAI DVRMPVAATA ETVDRFSTPE GHVGEGNVSM VIWTTTPWTI
260 270 280 290 300
PANRAVTLAE GLEYTLVQVE ASDDQPAERV ILADDLVNDC MERWNITHYH
310 320 330 340 350
KLGFCKGKDL EGLQLQHPLF ELQVPVILGD HVTTESGTGC VHTAPGHGVD
360 370 380 390 400
DFLVGQKYGI EVYNPVGDNG VYKDDTPLFA GQHVFKANEP IVDKLREVGN
410 420 430 440 450
LMHAESYRHS YPHCWRHKTP IIFRATPQWF ISMDKKGLRA GALEQIKKVD
460 470 480 490 500
WFPEWGQSRI ESMVEGRPDW CISRQRTWGN PIAIFVNRET EELHPNTLEL
510 520 530 540 550
MEKVAQLIEK DGVQAWFDLE PETLLGDEAD KYRKVTDTLD VWFDSGVTHH
560 570 580 590 600
CVLREFDGLR WPADLYLEGS DQHRGWFQSS LVTGVAMYDE APYHQVLTHG
610 620 630 640 650
FTVDAQGRKM SKSIGNVVAP QDVMNKLGGD ILRLWVASAD YSGEMTVSDE
660 670 680 690 700
ILKRSADAYR RIRNTSRFLL ANLSGFDPKQ DLVAKEDMVE LDRWIVGRAA
710 720 730 740 750
DLQNELLEAY NNYQFHSVVQ KLMHFCSIEL GSFYLDIIKD RQYTAKSEGL
760 770 780 790 800
ARRSCQTALY HIAEALVRWM APICSFTAQE IWDLLPNERS QYVFTESWYQ
810 820 830 840 850
GFDEFSGVKD AENEFWMQLL EIRDVVNQAL EQSRRDDVIG GSLQAEVTLY
860 870 880 890 900
ADDELAAALN RLGEELRFAL LTSEAQVASI NDAPADAVKA EKMALAVSVT
910 920 930 940
KSEYKKCERC WHHREEVGTL ENHSDLCQRC VTNIEGEGEE RRFA
Length:944
Mass (Da):107,332
Last modified:January 4, 2005 - v1
Checksum:i018253776C898182
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017340 Genomic DNA. Translation: AAV81968.1.
RefSeqiYP_155517.1. NC_006512.1.

Genome annotation databases

EnsemblBacteriaiAAV81968; AAV81968; IL1128.
GeneIDi3173785.
KEGGiilo:IL1128.
PATRICi22140315. VBIIdiLoi21852_1129.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017340 Genomic DNA. Translation: AAV81968.1 .
RefSeqi YP_155517.1. NC_006512.1.

3D structure databases

ProteinModelPortali Q5QZS6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 283942.IL1128.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAV81968 ; AAV81968 ; IL1128 .
GeneIDi 3173785.
KEGGi ilo:IL1128.
PATRICi 22140315. VBIIdiLoi21852_1129.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci ILOI283942:GI0U-1133-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-735 / DSM 15497 / L2-TR.

Entry informationi

Entry nameiSYI_IDILO
AccessioniPrimary (citable) accession number: Q5QZS6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: January 4, 2005
Last modified: November 26, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3