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Q5QZS6 (SYI_IDILO) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Isoleucine--tRNA ligase

EC=6.1.1.5
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS
Gene names
Name:ileS
Ordered Locus Names:IL1128
OrganismIdiomarina loihiensis (strain ATCC BAA-735 / DSM 15497 / L2-TR) [Complete proteome] [HAMAP]
Taxonomic identifier283942 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesIdiomarinaceaeIdiomarina

Protein attributes

Sequence length944 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP-Rule MF_02002

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP-Rule MF_02002

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_02002

Subunit structure

Monomer By similarity. HAMAP-Rule MF_02002

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_02002.

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP-Rule MF_02002

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 944944Isoleucine--tRNA ligase HAMAP-Rule MF_02002
PRO_0000098400

Regions

Motif58 – 6811"HIGH" region HAMAP-Rule MF_02002
Motif609 – 6135"KMSKS" region HAMAP-Rule MF_02002

Sites

Metal binding9071Zinc By similarity
Metal binding9101Zinc By similarity
Metal binding9271Zinc By similarity
Metal binding9301Zinc By similarity
Binding site5681Aminoacyl-adenylate By similarity
Binding site6121ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5QZS6 [UniParc].

Last modified January 4, 2005. Version 1.
Checksum: 018253776C898182

FASTA944107,332
        10         20         30         40         50         60 
MSDYKHTLNL PETAFPMRGN LAQREPKMLE QWYETDLYGQ IRKAKAGKPK FILHDGPPYA 

        70         80         90        100        110        120 
NGQIHIGHAV NKILKDVIVK AKTLSDFDAP YVPGWDCHGL PIELQVEKKH GKPGKKLNLA 

       130        140        150        160        170        180 
EFREKCREYA MTQIDQQRTD FKRLGVLGDW DNPYLTMNFK QEADSVRALA KIIDNGHMHQ 

       190        200        210        220        230        240 
GFKPVHWCTD CGSALAEAEV EYQDKNSPAI DVRMPVAATA ETVDRFSTPE GHVGEGNVSM 

       250        260        270        280        290        300 
VIWTTTPWTI PANRAVTLAE GLEYTLVQVE ASDDQPAERV ILADDLVNDC MERWNITHYH 

       310        320        330        340        350        360 
KLGFCKGKDL EGLQLQHPLF ELQVPVILGD HVTTESGTGC VHTAPGHGVD DFLVGQKYGI 

       370        380        390        400        410        420 
EVYNPVGDNG VYKDDTPLFA GQHVFKANEP IVDKLREVGN LMHAESYRHS YPHCWRHKTP 

       430        440        450        460        470        480 
IIFRATPQWF ISMDKKGLRA GALEQIKKVD WFPEWGQSRI ESMVEGRPDW CISRQRTWGN 

       490        500        510        520        530        540 
PIAIFVNRET EELHPNTLEL MEKVAQLIEK DGVQAWFDLE PETLLGDEAD KYRKVTDTLD 

       550        560        570        580        590        600 
VWFDSGVTHH CVLREFDGLR WPADLYLEGS DQHRGWFQSS LVTGVAMYDE APYHQVLTHG 

       610        620        630        640        650        660 
FTVDAQGRKM SKSIGNVVAP QDVMNKLGGD ILRLWVASAD YSGEMTVSDE ILKRSADAYR 

       670        680        690        700        710        720 
RIRNTSRFLL ANLSGFDPKQ DLVAKEDMVE LDRWIVGRAA DLQNELLEAY NNYQFHSVVQ 

       730        740        750        760        770        780 
KLMHFCSIEL GSFYLDIIKD RQYTAKSEGL ARRSCQTALY HIAEALVRWM APICSFTAQE 

       790        800        810        820        830        840 
IWDLLPNERS QYVFTESWYQ GFDEFSGVKD AENEFWMQLL EIRDVVNQAL EQSRRDDVIG 

       850        860        870        880        890        900 
GSLQAEVTLY ADDELAAALN RLGEELRFAL LTSEAQVASI NDAPADAVKA EKMALAVSVT 

       910        920        930        940 
KSEYKKCERC WHHREEVGTL ENHSDLCQRC VTNIEGEGEE RRFA 

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References

[1]"Genome sequence of the deep-sea gamma-proteobacterium Idiomarina loihiensis reveals amino acid fermentation as a source of carbon and energy."
Hou S., Saw J.H., Lee K.S., Freitas T.A., Belisle C., Kawarabayasi Y., Donachie S.P., Pikina A., Galperin M.Y., Koonin E.V., Makarova K.S., Omelchenko M.V., Sorokin A., Wolf Y.I., Li Q.X., Keum Y.S., Campbell S., Denery J. expand/collapse author list , Aizawa S., Shibata S., Malahoff A., Alam M.
Proc. Natl. Acad. Sci. U.S.A. 101:18036-18041(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-735 / DSM 15497 / L2-TR.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017340 Genomic DNA. Translation: AAV81968.1.
RefSeqYP_155517.1. NC_006512.1.

3D structure databases

ProteinModelPortalQ5QZS6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING283942.IL1128.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAV81968; AAV81968; IL1128.
GeneID3173785.
KEGGilo:IL1128.
PATRIC22140315. VBIIdiLoi21852_1129.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0060.
HOGENOMHOG000246402.
KOK01870.
OMAVLGDWDN.
OrthoDBEOG644ZM1.

Enzyme and pathway databases

BioCycILOI283942:GI0U-1133-MONOMER.

Family and domain databases

Gene3D3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_02002. Ile_tRNA_synth_type1.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00392. ileS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYI_IDILO
AccessionPrimary (citable) accession number: Q5QZS6
Entry history
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: January 4, 2005
Last modified: February 19, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries