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Q5QZS6

- SYI_IDILO

UniProt

Q5QZS6 - SYI_IDILO

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Idiomarina loihiensis (strain ATCC BAA-735 / DSM 15497 / L2-TR)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 1 (04 Jan 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei568 – 5681Aminoacyl-adenylateUniRule annotation
    Binding sitei612 – 6121ATPUniRule annotation
    Metal bindingi907 – 9071ZincUniRule annotation
    Metal bindingi910 – 9101ZincUniRule annotation
    Metal bindingi927 – 9271ZincUniRule annotation
    Metal bindingi930 – 9301ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciILOI283942:GI0U-1133-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:IL1128
    OrganismiIdiomarina loihiensis (strain ATCC BAA-735 / DSM 15497 / L2-TR)
    Taxonomic identifieri283942 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesIdiomarinaceaeIdiomarina
    ProteomesiUP000001171: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 944944Isoleucine--tRNA ligasePRO_0000098400Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi283942.IL1128.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5QZS6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi58 – 6811"HIGH" regionAdd
    BLAST
    Motifi609 – 6135"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5QZS6-1 [UniParc]FASTAAdd to Basket

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    MSDYKHTLNL PETAFPMRGN LAQREPKMLE QWYETDLYGQ IRKAKAGKPK    50
    FILHDGPPYA NGQIHIGHAV NKILKDVIVK AKTLSDFDAP YVPGWDCHGL 100
    PIELQVEKKH GKPGKKLNLA EFREKCREYA MTQIDQQRTD FKRLGVLGDW 150
    DNPYLTMNFK QEADSVRALA KIIDNGHMHQ GFKPVHWCTD CGSALAEAEV 200
    EYQDKNSPAI DVRMPVAATA ETVDRFSTPE GHVGEGNVSM VIWTTTPWTI 250
    PANRAVTLAE GLEYTLVQVE ASDDQPAERV ILADDLVNDC MERWNITHYH 300
    KLGFCKGKDL EGLQLQHPLF ELQVPVILGD HVTTESGTGC VHTAPGHGVD 350
    DFLVGQKYGI EVYNPVGDNG VYKDDTPLFA GQHVFKANEP IVDKLREVGN 400
    LMHAESYRHS YPHCWRHKTP IIFRATPQWF ISMDKKGLRA GALEQIKKVD 450
    WFPEWGQSRI ESMVEGRPDW CISRQRTWGN PIAIFVNRET EELHPNTLEL 500
    MEKVAQLIEK DGVQAWFDLE PETLLGDEAD KYRKVTDTLD VWFDSGVTHH 550
    CVLREFDGLR WPADLYLEGS DQHRGWFQSS LVTGVAMYDE APYHQVLTHG 600
    FTVDAQGRKM SKSIGNVVAP QDVMNKLGGD ILRLWVASAD YSGEMTVSDE 650
    ILKRSADAYR RIRNTSRFLL ANLSGFDPKQ DLVAKEDMVE LDRWIVGRAA 700
    DLQNELLEAY NNYQFHSVVQ KLMHFCSIEL GSFYLDIIKD RQYTAKSEGL 750
    ARRSCQTALY HIAEALVRWM APICSFTAQE IWDLLPNERS QYVFTESWYQ 800
    GFDEFSGVKD AENEFWMQLL EIRDVVNQAL EQSRRDDVIG GSLQAEVTLY 850
    ADDELAAALN RLGEELRFAL LTSEAQVASI NDAPADAVKA EKMALAVSVT 900
    KSEYKKCERC WHHREEVGTL ENHSDLCQRC VTNIEGEGEE RRFA 944
    Length:944
    Mass (Da):107,332
    Last modified:January 4, 2005 - v1
    Checksum:i018253776C898182
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017340 Genomic DNA. Translation: AAV81968.1.
    RefSeqiYP_155517.1. NC_006512.1.

    Genome annotation databases

    EnsemblBacteriaiAAV81968; AAV81968; IL1128.
    GeneIDi3173785.
    KEGGiilo:IL1128.
    PATRICi22140315. VBIIdiLoi21852_1129.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017340 Genomic DNA. Translation: AAV81968.1 .
    RefSeqi YP_155517.1. NC_006512.1.

    3D structure databases

    ProteinModelPortali Q5QZS6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 283942.IL1128.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAV81968 ; AAV81968 ; IL1128 .
    GeneIDi 3173785.
    KEGGi ilo:IL1128.
    PATRICi 22140315. VBIIdiLoi21852_1129.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci ILOI283942:GI0U-1133-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-735 / DSM 15497 / L2-TR.

    Entry informationi

    Entry nameiSYI_IDILO
    AccessioniPrimary (citable) accession number: Q5QZS6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: January 4, 2005
    Last modified: October 1, 2014
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3