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Q5QYD1 (SYL_IDILO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:IL0947
OrganismIdiomarina loihiensis (strain ATCC BAA-735 / DSM 15497 / L2-TR) [Complete proteome] [HAMAP]
Taxonomic identifier283942 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesIdiomarinaceaeIdiomarina

Protein attributes

Sequence length858 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 858858Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000152028

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif619 – 6235"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6221ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5QYD1 [UniParc].

Last modified January 4, 2005. Version 1.
Checksum: 7B0AF70F280A5176

FASTA85897,330
        10         20         30         40         50         60 
MQEQYNPSKI ESAMQKRWQE QDVFTAKEQP GKDKFYCLSM FPYPSGKLHM GHVRNYTLGD 

        70         80         90        100        110        120 
VISRHQRMLG KNVMQPMGWD AFGLPAENAA IQNKTAPAKW TYQNIDYMRE QLKSLGFGYD 

       130        140        150        160        170        180 
WNRELATCSP DYYRWEQWFF TKLYEKGLVY KKNATVNWDP VDQTVLANEQ VIDGRGWRSG 

       190        200        210        220        230        240 
AKVEQKEIPQ WFIKITDYAE ELLDDLDQLD GWPEQVKAMQ RNWIGRSEGV EIDFNVASSE 

       250        260        270        280        290        300 
QTLRVYTTRP DTLYGVTYMG VAAQHPLATE AAKTNPELAK FIEECKNSKV AEADIATMEK 

       310        320        330        340        350        360 
LGMDTGIKAV HPMTGEEIPV WVANFVLMDY GSGAVMAVPA HDQRDWEFAT KYQLEIRPVI 

       370        380        390        400        410        420 
EPLSGDSDIE KAAITEKGTV INSGPYNGMS SAQAFDAIAT ELKEKGIGER KVNYRLRDWG 

       430        440        450        460        470        480 
VSRQRYWGTP IPMLNLENGE SVPVPEDQLP VKLPEDVVMD GVNSPIKSDP EWRKTEYNGQ 

       490        500        510        520        530        540 
PAEHETDTFD TFMESSWYYA RYCSAQTDDA MLDPEKANYW LPVDQYIGGI EHAILHLLYA 

       550        560        570        580        590        600 
RFFHKLLRDT GLVESDEPFK RLLCQGMVLA DSYYREDEKG GKQWVSPLEV DIERDDKGAI 

       610        620        630        640        650        660 
AGAKHKQDGQ PVNIGGMSKM SKSKNNGIDP QTMVERYGAD TVRLFMMFAA PPEMTLEWSD 

       670        680        690        700        710        720 
SGVEGAQRFL RRLWKLTYEL NNAGGACSGQ SLNANQKQLR RELHKTIAKV SDDMGRRQHF 

       730        740        750        760        770        780 
NTAIAAIMEL LNHLQKAPLE SEADRQVLAE SIDATVRMLA PITPHICEQL WQELGHQEPL 

       790        800        810        820        830        840 
SFADWPAVDE SALVEDEKLI VVQINGKVRA KLTVPADASA EQVEQIAFDE EAVQKHTEGK 

       850 
DVRKKIYVPG KILNIVVG 

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References

[1]"Genome sequence of the deep-sea gamma-proteobacterium Idiomarina loihiensis reveals amino acid fermentation as a source of carbon and energy."
Hou S., Saw J.H., Lee K.S., Freitas T.A., Belisle C., Kawarabayasi Y., Donachie S.P., Pikina A., Galperin M.Y., Koonin E.V., Makarova K.S., Omelchenko M.V., Sorokin A., Wolf Y.I., Li Q.X., Keum Y.S., Campbell S., Denery J. expand/collapse author list , Aizawa S., Shibata S., Malahoff A., Alam M.
Proc. Natl. Acad. Sci. U.S.A. 101:18036-18041(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-735 / DSM 15497 / L2-TR.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017340 Genomic DNA. Translation: AAV81787.1.
RefSeqYP_155336.1. NC_006512.1.

3D structure databases

ProteinModelPortalQ5QYD1.
SMRQ5QYD1. Positions 228-412.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING283942.IL0947.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAV81787; AAV81787; IL0947.
GeneID3173530.
KEGGilo:IL0947.
PATRIC22139937. VBIIdiLoi21852_0949.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycILOI283942:GI0U-948-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_IDILO
AccessionPrimary (citable) accession number: Q5QYD1
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: January 4, 2005
Last modified: May 14, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries