Reviewed, UniProtKB/Swiss-Prot Q5QXQ2 (SYQ_IDILO)

Names and origin

Protein names

Recommended name:
    Glutaminyl-tRNA synthetase
    EC=6.1.1.18
Alternative name(s):
    Glutamine--tRNA ligase
      Short name=GlnRS

Gene names

Name:	glnS
Ordered Locus Names:	IL1478

Organism

Idiomarina loihiensis [Complete proteome] [HAMAP]

Taxonomic identifier

135577 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Idiomarinaceae › Idiomarina

Protein attributes

Sequence length	561 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln). HAMAP MF_00126
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutaminyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 561	561	Glutaminyl-tRNA synthetase HAMAP MF_00126		PRO_1000095492
Regions
	Motif	34 – 44	11	"HIGH" region HAMAP MF_00126
	Motif	268 – 272	5	"KMSKS" region HAMAP MF_00126
Sites
	Binding site	271	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5QXQ2-1 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: E48E5B3DFE2AF8F2	FASTA	561	64,529
	10 20 30 40 50 60 MADTDSRPSN FIRQIIDKDL ASGKHATVHT RFPPEPNGYL HIGHAKSIVL NFGIAEDYNG 70 80 90 100 110 120 TCNLRFDDTN PLKEKVDYVN SIKKDVEWLG YHWEGEPRYS SNYFDQLHGF AVELIEKGLA 130 140 150 160 170 180 YVDFSSQDKM REMRGTLKEP GVNSPYRDTS VEENLKHFAD MTAGKHEEGT AALRAKIDMS 190 200 210 220 230 240 SPFMCMRDPV IYRVRFVHHH QTGDKWCVYP MYDFTHCISD ALEGITHSLC TLEFQDNRRL 250 260 270 280 290 300 YDWVLDNISI DCHPQQIEFS RLNLQYTVMS KRIINTLVDE NKVSGWDDPR IASIAGLRRR 310 320 330 340 350 360 GYTPDSVREF CRRIGVTKMD NQVEMSMLEA CIRDDLNVNA PRAMAVMDPV KIVIENYPEG 370 380 390 400 410 420 EQELLDAPNH PNDPEMGSRQ VTFSREIWIE REDFRESANK KFKRLVLDKE VRLRNAYVIR 430 440 450 460 470 480 ADRIETDDNG EIQTIYCHYD ADTLGKDPAD GRKVKGVIHW VSAETAKAAE FRVYDRLFQV 490 500 510 520 530 540 PNPAAEEDLF STLNPESLVI KKGFVEANLA SAKLGENFQF ERLGYYCLDQ DAETEGRLIF 550 560 NQTVGLRDSW AKIEQEQTTG S

References

[1]

"Genome sequence of the deep-sea gamma-proteobacterium Idiomarina loihiensis reveals amino acid fermentation as a source of carbon and energy." Hou S., Saw J.H., Lee K.S., Freitas T.A., Belisle C., Kawarabayasi Y., Donachie S.P., Pikina A., Galperin M.Y., Koonin E.V., Makarova K.S., Omelchenko M.V., Sorokin A., Wolf Y.I., Li Q.X., Keum Y.S., Campbell S., Denery J. , Aizawa S., Shibata S., Malahoff A., Alam M. Proc. Natl. Acad. Sci. U.S.A. 101:18036-18041(2004) [PubMed: 15596722] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: L2-TR / ATCC BAA-735 / DSM 15497.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017340 Genomic DNA. Translation: AAV82318.1.
RefSeq	YP_155867.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3172053.
GenomeReviews	Gene locus IL1478 in contig AE017340_GR.
KEGG	ilo:IL1478.
NMPDR	fig|283942.3.peg.1928.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5QXQ2.
OMA	RMPTIAG.
Enzyme and pathway databases
BioCyc	ILOI283942:IL1478-MON.
Family and domain databases
HAMAP	MF_00126. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR004514. Gln-tRNA-synth_Ic. IPR000924. Glu/Gln-tRNA-synth_Ic. IPR020061. Glu/Gln-tRNA-synth_Ic_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ic_cat-dom. IPR020059. Glu/Gln-tRNA-synth_Ic_codon-bd. IPR020060. Glu/Gln-tRNA-synth_Ic_N. IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:2.40.240.10. Rbsml_L25/Gln-tRNA_synth_b-brl. 1 hit.
PANTHER	PTHR10119:SF3. GlnS. 1 hit. PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. PF03950. tRNA-synt_1c_C. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
TIGRFAMs	TIGR00440. glnS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYQ_IDILO

Accession

Primary (citable) accession number: Q5QXQ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	January 4, 2005
Last modified:	November 3, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)