Reviewed, UniProtKB/Swiss-Prot Q5QXC3 (KATG1_IDILO)

Names and origin

Protein names

Recommended name:
    Catalase-peroxidase 1
      Short name=CP 1
    EC=1.11.1.6
    EC=1.11.1.7
Alternative name(s):
    Peroxidase/catalase 1

Gene names

Name:	katG1
Ordered Locus Names:	IL0108

Organism

Idiomarina loihiensis [Complete proteome] [HAMAP]

Taxonomic identifier

135577 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Idiomarinaceae › Idiomarina

Protein attributes

Sequence length	725 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity.
Catalytic activity	2 H2O2 = O2 + 2 H2O. HAMAP MF_01961 Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity.
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity.
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: HAMAP heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 725	725	Catalase-peroxidase 1 HAMAP MF_01961		PRO_0000354810
Sites
	Active site	97	1	Proton acceptor By similarity
	Metal binding	265	1	Iron (heme axial ligand) By similarity
	Site	93	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	96 ↔ 224		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-250) By similarity
	Cross-link	224 ↔ 250		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-96) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5QXC3-1 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: EE0F81E06C4F532E	FASTA	725	82,347
	10 20 30 40 50 60 MTKSSGGGKC PIMHGSMTSE KKTNTHWWPN SLKLEILRQH DTKANPLDED FNYAEEFKKI 70 80 90 100 110 120 NLEELKQDLK NFMTDSQEWW PADWGHYGGL MIRMAWHSAG SYRLADGRGG GGTGNQRFAP 130 140 150 160 170 180 LNSWPDNVSL DKARRLLWPI KKKYGNKLSW ADLFILAGNM AYESMGLKTF GFAGGREDIW 190 200 210 220 230 240 HPEEDTYWGS EKEWLAETKN RYSSDEERDS LENPLAAVQM GLIYVNPEGV DGNPDPLRTA 250 260 270 280 290 300 KDVRETFKRM AMNDEETCAL TAGGHTVGKC HGNGKEENLG PEPEAADVEE QGMGWRNSGG 310 320 330 340 350 360 KGVGRDTMSS GIEGAWTTNP TQWDNGYFYL LFNYEWELKK SPAGAWQWEP IDIKEEDKPV 370 380 390 400 410 420 DVEDPSIRHN PIMTDADMAM KMDPDYRKIA EKFYKDPEYF ADVFARAWFK LTHRDLGPKD 430 440 450 460 470 480 RYLGADVPEE DLIWQDPIPK VDYSLSEQEI ADLKEKLLNT ELTSYELIST AWDSARNFRC 490 500 510 520 530 540 SDFRGGANGA RIRLAPQKDW HGNEPEKLHK VLRVLEDLQS TLDKKVSLAD LIVLGGTAAV 550 560 570 580 590 600 EKAAKDAGYN VTVPFAPGRG DATDEQTDVE SFEPLEPLHD GYRNYLKKDY AVPAEELMLD 610 620 630 640 650 660 RTHLMGLTAP EMTVLVGGMR ALGANYGESE HGVFTDRVGT LTNDFFVNLT DMNNVWKKAD 670 680 690 700 710 720 DHYEVRDRKT DELKWTASRI DLVFGSNSIL RSYSELYAQD DNQEKFVKDF VKAWTKVMNA DRFDL

References

[1]

"Genome sequence of the deep-sea gamma-proteobacterium Idiomarina loihiensis reveals amino acid fermentation as a source of carbon and energy." Hou S., Saw J.H., Lee K.S., Freitas T.A., Belisle C., Kawarabayasi Y., Donachie S.P., Pikina A., Galperin M.Y., Koonin E.V., Makarova K.S., Omelchenko M.V., Sorokin A., Wolf Y.I., Li Q.X., Keum Y.S., Campbell S., Denery J. , Aizawa S., Shibata S., Malahoff A., Alam M. Proc. Natl. Acad. Sci. U.S.A. 101:18036-18041(2004) [PubMed: 15596722] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: L2-TR / ATCC BAA-735 / DSM 15497.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017340 Genomic DNA. Translation: AAV80951.1.
RefSeq	YP_154500.1.
3D structure databases
ModBase	Search...
Protein family/group databases
PeroxiBase	2638. IlCP02.
Genome annotation databases
GeneID	3174398.
GenomeReviews	Gene locus IL0108 in contig AE017340_GR.
KEGG	ilo:IL0108.
NMPDR	fig|283942.3.peg.179.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5QXC3.
OMA	Q5QXC3. WPNALNL.
Enzyme and pathway databases
BioCyc	ILOI283942:IL0108-MON.
Family and domain databases
HAMAP	MF_01961. [Tree]
InterPro	IPR000763. Catalase_proxase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
TIGRFAMs	TIGR00198. cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. False negative. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG1_IDILO

Accession

Primary (citable) accession number: Q5QXC3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	January 4, 2005
Last modified:	May 5, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)