Reviewed, UniProtKB/Swiss-Prot Q5QWY0 (PUR4_IDILO)

Names and origin

Protein names

Recommended name:
    Phosphoribosylformylglycinamidine synthase
      Short name=FGAM synthase
      Short name=FGAMS
    EC=6.3.5.3
Alternative name(s):
    Formylglycinamide ribotide amidotransferase
      Short name=FGARAT
    Formylglycinamide ribotide synthetase

Gene names

Name:	purL
Ordered Locus Names:	IL0591

Organism

Idiomarina loihiensis [Complete proteome] [HAMAP]

Taxonomic identifier

135577 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Idiomarinaceae › Idiomarina

Protein attributes

Sequence length	1295 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00419
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00419
Subunit structure	Monomer. HAMAP MF_00419
Subcellular location	Cytoplasm HAMAP MF_00419.
Sequence similarities	In the N-terminal section; belongs to the FGAMS family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1295	1295	Phosphoribosylformylglycinamidine synthase HAMAP MF_00419		PRO_0000264578
Regions
	Domain	1042 – 1295	254	Glutamine amidotransferase type-1
	Nucleotide binding	305 – 316	12	ATP Potential
Sites
	Active site	1135	1	Nucleophile By similarity
	Active site	1260	1	By similarity
	Active site	1262	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5QWY0-1 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: D08A563F6584A26F	FASTA	1,295	142,005
	10 20 30 40 50 60 MEIYRGAPAL SAFKTTKQLE QLKQAGIPVK ELYAEYQHFV DLHNELSDEH RSVLVQLLKY 70 80 90 100 110 120 GPEMPAHEPQ GALVLVTPRI GTISPWASKA TDIAHNCGLK SIHRVERGVA FYLQGDLSAE 130 140 150 160 170 180 ELKQAALLLH DRMTESVLYD MNDAQQLFRS QEPQPLSSVD ILAGGREALA QANISLGLAL 190 200 210 220 230 240 ADDEIDYLVE NFRKLDRNPN DIELYMFAQA NSEHCRHKIF NADWTIDGAE QPKSLFKMIK 250 260 270 280 290 300 NTFETTPDYV LSAYKDNAAV MEGHEAGRFY PQPDSMSYGY SHEPVHILMK VETHNHPTAI 310 320 330 340 350 360 SPYPGAATGS GGEIRDEGAT GVGSKPKAGL VGFSVSNLNI PGFKQPWEEN YGKPARIVSA 370 380 390 400 410 420 LDIMLEGPLG GAAFNNEFGR PALTGYFRTY EQTVDSHNGR ETRGYHKPIM IAGGLGNIRE 430 440 450 460 470 480 AHVQKGDIPV GAKLVVLGGP AMNIGLGGGA ASSMASGEST EDLDFASVQR ENPEMERRCQ 490 500 510 520 530 540 EVIDRCWQLG ADNPIAFIHD VGAGGLSNAM PELVSDGGRG GRFELREIPN DEPGMTPLEI 550 560 570 580 590 600 WCNESQERYV IAIAPENLAR FEALCERERA EYAVIGEATE ELTILLNDAK FSNQPIDLSL 610 620 630 640 650 660 DVLLGKPPKM HRDVARLQTE GTPLHLEAAD LNDAADRLLR LPAIAEKTFL ITIGDRSVTG 670 680 690 700 710 720 LVARDQMVGP WQIPVADVAV TASSYDSYHG EAMAMGERTP LALLNFGASA RMAVAESLTN 730 740 750 760 770 780 IAAADIGDLK RIKLSANWMC AAGHPGEDAG LYEAVKAVGE ELCPELGITI PVGKDSMSMK 790 800 810 820 830 840 TQWQQDGEDK AVTAPMSLVI TAFGRVNDIR STLTPQLRTD KGQSHLVLID LGKGQNRLGG 850 860 870 880 890 900 SALAQVYQQL GQHTPDLDDT ETFKAFFNTT QQLVTEGRLL AYHDRSDGGL FTTVAEMAFA 910 920 930 940 950 960 GNCGAKVALD ELGEDNLATL FNEELGAVIQ VSDEQYQKVM DAYKTAGLGD CVKRIGEPTH 970 980 990 1000 1010 1020 EDAIVFTRDE QNVLAQSRTH WRTVWAETTH HMQRLRDNPV CADEEFRLKQ RADNPGLLAD 1030 1040 1050 1060 1070 1080 LTFDPSEDIA APYIAKGVAP KVAILREQGV NSHYEMAAAF DRAGFEAVDV HMSDILAGRV 1090 1100 1110 1120 1130 1140 SLEDMQALAA CGGFSYGDVL GAGEGWAKSI LFNDRAREQF EAFFKRNDTL ALGVCNGCQM 1150 1160 1170 1180 1190 1200 LSTLKQLIPG TEHWPRFVTN RSERFEARFS LVEVQESKSI FLGDMAGSRM PIAVSHGEGR 1210 1220 1230 1240 1250 1260 AEFANPQQQS QLEQNSQVAL RYIDNWGEVA EQYPANPNGS PKGITAVTSD DGRVTAMMPH 1270 1280 1290 PERVFRTVAN SWHPDEWGED SPWMRMFRNA RKHLG

References

[1]

"Genome sequence of the deep-sea gamma-proteobacterium Idiomarina loihiensis reveals amino acid fermentation as a source of carbon and energy." Hou S., Saw J.H., Lee K.S., Freitas T.A., Belisle C., Kawarabayasi Y., Donachie S.P., Pikina A., Galperin M.Y., Koonin E.V., Makarova K.S., Omelchenko M.V., Sorokin A., Wolf Y.I., Li Q.X., Keum Y.S., Campbell S., Denery J. , Aizawa S., Shibata S., Malahoff A., Alam M. Proc. Natl. Acad. Sci. U.S.A. 101:18036-18041(2004) [PubMed: 15596722] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: L2-TR / ATCC BAA-735 / DSM 15497.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017340 Genomic DNA. Translation: AAV81432.1.
RefSeq	YP_154981.1.
3D structure databases
HSSP	HSSP built from PDB template 1T3T based on UniProtKB P74881.
ModBase	Search...
Genome annotation databases
GeneID	3173305.
GenomeReviews	Gene locus IL0591 in contig AE017340_GR.
KEGG	ilo:IL0591.
NMPDR	fig|283942.3.peg.542.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG335309.
OMA	ERGIAYY.
Enzyme and pathway databases
BioCyc	ILOI283942:IL0591-MONOMER.
BRENDA	6.3.5.3. 280818.
Family and domain databases
HAMAP	MF_00419. PurL_1. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR017926. GATASE_1. IPR010073. PRibForGlyAmidine_synth. IPR016188. PurM_N-like. [Graphical view]
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 2 hits. [Graphical view]
TIGRFAMs	TIGR01735. FGAM_synt. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR4_IDILO

Accession

Primary (citable) accession number: Q5QWY0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	January 4, 2005
Last modified:	February 9, 2010
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)