Reviewed, UniProtKB/Swiss-Prot Q5QWC6 (PYRC_IDILO)

Names and origin

Protein names

Recommended name:
    Dihydroorotase
      Short name=DHOase
    EC=3.5.2.3

Gene names

Name:	pyrC
Ordered Locus Names:	IL0362

Organism

Idiomarina loihiensis [Complete proteome] [HAMAP]

Taxonomic identifier

135577 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Idiomarinaceae › Idiomarina

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + H(2)O = N-carbamoyl-L-aspartate.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 3/6.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the DHOase family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	dihydroorotase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 344	344	Dihydroorotase		PRO_1000024018
Sites
	Metal binding	13	1	Zinc 1 By similarity
	Metal binding	15	1	Zinc 1 By similarity
	Metal binding	99	1	Zinc 1; via carbamate group By similarity
	Metal binding	99	1	Zinc 2; via carbamate group By similarity
	Metal binding	136	1	Zinc 2 By similarity
	Metal binding	174	1	Zinc 2 By similarity
	Metal binding	247	1	Zinc 1 By similarity
Amino acid modifications
	Modified residue	99	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5QWC6-1 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: 0524427E85F4BD41	FASTA	344	37,656
	10 20 30 40 50 60 MQSLTIPRPD DWHLHLRDGA MLQTTVPATA AVFHRAVVMP NLVPPVTTVA AAMEYRERIL 70 80 90 100 110 120 AEIPAGMSFN PLMALYLTAD TTPAEIAEAA ANEHVIGFKL YPSGATTNSA AGVKSVEALA 130 140 150 160 170 180 PVLAAMQEHQ VPLLVHGEVT DSDIDIFDRE RVFIERHLIP ITERFPQLKL VLEHITTADA 190 200 210 220 230 240 VKFVENANSN VAATMTPQHL LMNRNDLLVG GIRPHNYCLP ILKRRSHQQA LQQAALSGNP 250 260 270 280 290 300 KFFLGTDSAP HVQANKETAC GCAGCYSAPA AIELYAEFFS QHNALDKLAN FASVFGADFY 310 320 330 340 QLPRNTETIE LVQQPWTVAE TVDTATGPMV PYWAGSDLQW KLRG

References

[1]

"Genome sequence of the deep-sea gamma-proteobacterium Idiomarina loihiensis reveals amino acid fermentation as a source of carbon and energy." Hou S., Saw J.H., Lee K.S., Freitas T.A., Belisle C., Kawarabayasi Y., Donachie S.P., Pikina A., Galperin M.Y., Koonin E.V., Makarova K.S., Omelchenko M.V., Sorokin A., Wolf Y.I., Li Q.X., Keum Y.S., Campbell S., Denery J. , Aizawa S., Shibata S., Malahoff A., Alam M. Proc. Natl. Acad. Sci. U.S.A. 101:18036-18041(2004) [PubMed: 15596722] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: L2-TR / ATCC BAA-735 / DSM 15497.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017340 Genomic DNA. Translation: AAV81205.1.
RefSeq	YP_154754.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3172502.
GenomeReviews	Gene locus IL0362 in contig AE017340_GR.
KEGG	ilo:IL0362.
NMPDR	fig|283942.3.peg.446.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5QWC6.
Enzyme and pathway databases
BioCyc	ILOI283942:IL0362-MON.
Family and domain databases
HAMAP	MF_00219. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR004721. DHOdimr. IPR002195. Dihydroorotase_CS. [Graphical view]
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
PIRSF	PIRSF001237. DHOdimr. 1 hit.
TIGRFAMs	TIGR00856. pyrC_dimer. 1 hit.
PROSITE	PS00482. DIHYDROOROTASE_1. 1 hit. PS00483. DIHYDROOROTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRC_IDILO

Accession

Primary (citable) accession number: Q5QWC6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 4, 2005
Last modified:	November 25, 2008
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)