Q5QSK2 (TM11G_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transmembrane protease serine 11G
EC=3.4.21.-

Alternative name(s):
Serine protease DESC4

Cleaved into the following 2 chains:

1. Transmembrane protease serine 11G non-catalytic chain
2. Transmembrane protease serine 11G catalytic chain

Gene names

Name:	Tmprss11g
Synonyms:	Desc4

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	417 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Subcellular location	Membrane; Single-pass type II membrane protein Ref.1.
Tissue specificity	Highest expression in lung and tongue. Also expressed in brain, colon, heart and liver. Isoform 1 is the predominant form in tongue whereas both isoforms are expressed in similar amounts in lung. At the cellular level, expression is confined to epithelial cells within the cleft of the circumvallate papillae extending into the ducts of the minor salivary glands, the respiratory epithelium of the nasal cavity and tear gland ducts. Ref.1
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain. Contains 1 SEA domain.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: InterPro integral to plasma membrane Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from direct assay Ref.1. Source: UniProtKB
Molecular_function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 Ref.1 (identifier: Q5QSK2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 Ref.1 (identifier: Q5QSK2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     160-173: EMNAAQAEHILNSN → D

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 185	185	Transmembrane protease serine 11G non-catalytic chain Potential Ref.1		PRO_0000027839
	Chain	186 – 417	232	Transmembrane protease serine 11G catalytic chain Potential Ref.1		PRO_0000027840
Regions
	Topological domain	1 – 22	22	Cytoplasmic Potential
	Transmembrane	23 – 43	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	44 – 417	374	Extracellular Potential
	Domain	58 – 165	108	SEA
	Domain	186 – 416	231	Peptidase S1
Sites
	Active site	226	1	Charge relay system By similarity UniProtKB P10144
	Active site	271	1	Charge relay system By similarity UniProtKB P10144
	Active site	367	1	Charge relay system By similarity UniProtKB P10144
Amino acid modifications
	Disulfide bond	211 ↔ 227		By similarity UniProtKB P49863
	Disulfide bond	336 ↔ 352		By similarity UniProtKB P49863
	Disulfide bond	363 ↔ 392		By similarity UniProtKB P49863
Natural variations
	Alternative sequence	160 – 173	14	EMNAA…ILNSN → D in isoform 2. Ref.1		VSP_014182

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: 4B4380D78726FDA4	FASTA	417	46,593
	10 20 30 40 50 60 MYQPGILGRR KRVCKPWTVA LTTTAALLAL AVLIGLLVYF LVYEEKTHYY QASFWIPSIK 70 80 90 100 110 120 YSSDLSEEQS KLQINLKQKI NNEIDVIFQR SSLKHHYVKS QVVNFRPSND GVKADILIKF 130 140 150 160 170 180 QIPRKNADTL RSEADSILNK KLQSSQSFLK RDISLPYLRE MNAAQAEHIL NSNCGLGMEY 190 200 210 220 230 240 PRIARIADGK PAGSNSWPWQ SSLQVEGIHL CGASLIGSQW LVTSAHCFDN YKNPKLWTVS 250 260 270 280 290 300 FGRTLGNPLT TRKVESIIIH ENYAAHKHDD DIAVVKLSSP VLFSENLRTV CLPEATFQVL 310 320 330 340 350 360 PKSKVFVTGW GALKANGPFP NSLQEVEIEI ISNDVCNQVN VYGGAISSGM ICAGFLTGKL 370 380 390 400 410 DACEGDSGGP LVISDNRNKW YLLGIVSWGI DCGKENKPGI YTRVTHYRNW IKSKTNI
	Isoform 2 [UniParc]. Checksum: 4E77309826E30AAA	FASTA	404	45,184
	10 20 30 40 50 60 MYQPGILGRR KRVCKPWTVA LTTTAALLAL AVLIGLLVYF LVYEEKTHYY QASFWIPSIK 70 80 90 100 110 120 YSSDLSEEQS KLQINLKQKI NNEIDVIFQR SSLKHHYVKS QVVNFRPSND GVKADILIKF 130 140 150 160 170 180 QIPRKNADTL RSEADSILNK KLQSSQSFLK RDISLPYLRD CGLGMEYPRI ARIADGKPAG 190 200 210 220 230 240 SNSWPWQSSL QVEGIHLCGA SLIGSQWLVT SAHCFDNYKN PKLWTVSFGR TLGNPLTTRK 250 260 270 280 290 300 VESIIIHENY AAHKHDDDIA VVKLSSPVLF SENLRTVCLP EATFQVLPKS KVFVTGWGAL 310 320 330 340 350 360 KANGPFPNSL QEVEIEIISN DVCNQVNVYG GAISSGMICA GFLTGKLDAC EGDSGGPLVI 370 380 390 400 SDNRNKWYLL GIVSWGIDCG KENKPGIYTR VTHYRNWIKS KTNI

References

[1]

"Molecular cloning and characterisation of DESC4, a new transmembrane serine protease." Behrens M., Bufe B., Schmale H., Meyerhof W. Cell. Mol. Life Sci. 61:2866-2877(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Strain: Wistar. Tissue: Circumvallate papilla.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ617481 mRNA. Translation: CAE84572.1. AJ617528 mRNA. Translation: CAE84986.1.
IPI	IPI00388183. IPI00734792.
RefSeq	NP_001008554.1. NM_001008554.1.
UniGene	Rn.136778.
3D structure databases
ProteinModelPortal	Q5QSK2.
SMR	Q5QSK2. Positions 186-417.
ModBase	Search...
Protein family/group databases
MEROPS	S01.436.
PTM databases
PhosphoSite	Q5QSK2.
Proteomic databases
PRIDE	Q5QSK2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	289546.
KEGG	rno:289546.
UCSC	RGD:1306446. rat.
Organism-specific databases
CTD	320454.
RGD	1306446. Tmprss11g.
Phylogenomic databases
eggNOG	COG5640.
HOGENOM	HOG000251823.
HOVERGEN	HBG013304.
InParanoid	Q5QSK2.
OrthoDB	EOG4W3SN6.
Gene expression databases
Genevestigator	Q5QSK2.
Family and domain databases
InterPro	IPR017329. Pept_S1A_HAT/DESC1. IPR001254. Peptidase_S1. IPR018114. Peptidase_S1_AS. IPR001314. Peptidase_S1A. IPR000082. SEA. IPR009003. Trypsin-like_Pept_dom. [Graphical view]
Pfam	PF01390. SEA. 1 hit. PF00089. Trypsin. 1 hit. [Graphical view]
PIRSF	PIRSF037941. TMPRSS11ABCDE. 1 hit.
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00200. SEA. 1 hit. SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50024. SEA. 1 hit. PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. False negative. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	629976.

Entry information

Entry name

TM11G_RAT

Accession

Primary (citable) accession number: Q5QSK2
Secondary accession number(s): Q5NJM5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	January 4, 2005
Last modified:	March 6, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program