Q5QP82 (DCA10_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DDB1- and CUL4-associated factor 10

Alternative name(s):
WD repeat-containing protein 32

Gene names

Name:	DCAF10
Synonyms:	WDR32

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	559 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex. Ref.5
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with DDB1. Ref.5
Sequence similarities	Belongs to the WD repeat DCAF10 family. Contains 7 WD repeats.
Sequence caution	The sequence AAI08687.1 differs from that shown. Reason: Erroneous initiation. The sequence BAB15574.1 differs from that shown. Reason: Erroneous initiation. The sequence BAC11043.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Coding sequence diversity	Alternative splicing
Domain	Repeat WD repeat
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein ubiquitination Inferred by curator Ref.5. Source: UniProtKB
Cellular component	CUL4 RING ubiquitin ligase complex Inferred from direct assay Ref.5. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q5QP82-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q5QP82-2)

The sequence of this isoform differs from the canonical sequence as follows:
     352-388: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 559	559	DDB1- and CUL4-associated factor 10		PRO_0000306833
Regions
	Repeat	166 – 205	40	WD 1
	Repeat	209 – 247	39	WD 2
	Repeat	251 – 290	40	WD 3
	Repeat	296 – 335	40	WD 4
	Repeat	408 – 448	41	WD 5
	Repeat	470 – 508	39	WD 6
	Repeat	526 – 559	34	WD 7
	Compositional bias	34 – 100	67	Pro-rich
	Compositional bias	349 – 385	37	Ser-rich
Amino acid modifications
	Modified residue	61	1	Phosphoserine Ref.6
Natural variations
	Alternative sequence	352 – 388	37	Missing in isoform 2.		VSP_028513
Experimental info
	Sequence conflict	309	1	D → G in BAC11043. Ref.4
	Sequence conflict	332	1	K → N in AAH03520. Ref.3
	Sequence conflict	457	1	C → W in BAB15574. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: 583B5A781ADAED38	FASTA	559	60,582
	10 20 30 40 50 60 MFPFGPHSPG GDGSAGAGAE EPTPHEGQAA ATGPPSPLHP GADATHPPPP ARSPRRPGAP 70 80 90 100 110 120 SLSPAPRSGE LGLPGAPESS TASAPGEPSP PSPPCRRPGP DCRAKSRGRH GLGAGLGGPG 130 140 150 160 170 180 ARLFGWLKER SLGRGLFVDP ARDNFRTMTS LYGSIHPADS VYLSTRTHGA VFNLEYSPDG 190 200 210 220 230 240 SVLTVACEQT EVLLFDPISS KHIKTLSEAH EDCVNNIRFL DNRLFATCSD DTTIALWDLR 250 260 270 280 290 300 KLNTKVCTLH GHTSWVKNIE YDTNTRLLVT SGFDGNVIIW DTNRYTEDGC PHKKFFHTRF 310 320 330 340 350 360 LMRMRLTPDC SKMLISTSSG YLLILHDLDL TKSLEVGSYP ILRARRTTSS SDLTTSSSSS 370 380 390 400 410 420 GPRVSGSPCH HSDSNSSEKH MSRASQREGV SPRNSLEVVT PEVLGESDHG NCITSLQLHP 430 440 450 460 470 480 KGWATLLRCS SNSDDEECTC VYEFQEGAPV RPVSPRCSLR LTHYIEEANV GRGYIKELCF 490 500 510 520 530 540 SPDGRMISSP HGYGIRLLGF DKQCSELVDC LPKEASPLRV IRSLYSHNDV VLTTKFSPTH 550 CQIASGCLSG RVSLYQPKF
	Isoform 2 [UniParc]. Checksum: 75DCBB1C9E20B368	FASTA	522	56,683
	10 20 30 40 50 60 MFPFGPHSPG GDGSAGAGAE EPTPHEGQAA ATGPPSPLHP GADATHPPPP ARSPRRPGAP 70 80 90 100 110 120 SLSPAPRSGE LGLPGAPESS TASAPGEPSP PSPPCRRPGP DCRAKSRGRH GLGAGLGGPG 130 140 150 160 170 180 ARLFGWLKER SLGRGLFVDP ARDNFRTMTS LYGSIHPADS VYLSTRTHGA VFNLEYSPDG 190 200 210 220 230 240 SVLTVACEQT EVLLFDPISS KHIKTLSEAH EDCVNNIRFL DNRLFATCSD DTTIALWDLR 250 260 270 280 290 300 KLNTKVCTLH GHTSWVKNIE YDTNTRLLVT SGFDGNVIIW DTNRYTEDGC PHKKFFHTRF 310 320 330 340 350 360 LMRMRLTPDC SKMLISTSSG YLLILHDLDL TKSLEVGSYP ILRARRTTSS SGVSPRNSLE 370 380 390 400 410 420 VVTPEVLGES DHGNCITSLQ LHPKGWATLL RCSSNSDDEE CTCVYEFQEG APVRPVSPRC 430 440 450 460 470 480 SLRLTHYIEE ANVGRGYIKE LCFSPDGRMI SSPHGYGIRL LGFDKQCSEL VDCLPKEASP 490 500 510 520 LRVIRSLYSH NDVVLTTKFS PTHCQIASGC LSGRVSLYQP KF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequence and analysis of human chromosome 9." Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I. Nature 429:369-374(2004) [PubMed: 15164053] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 298-559 (ISOFORM 2). Tissue: Ovary, Skin and Uterus.
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 145-559. Tissue: Embryo and Ileal mucosa.
[5]	"A family of diverse Cul4-Ddb1-interacting proteins includes Cdt2, which is required for S phase destruction of the replication factor Cdt1." Jin J., Arias E.E., Chen J., Harper J.W., Walter J.C. Mol. Cell 23:709-721(2006) [PubMed: 16949367] [Abstract] Cited for: FUNCTION, INTERACTION WITH DDB1, IDENTIFICATION BY MASS SPECTROMETRY.
[6]	"Large-scale proteomics analysis of the human kinome." Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G., Mann M., Daub H. Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed: 19369195] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-61, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL138752 Genomic DNA. Translation: CAI13878.1. CH471071 Genomic DNA. Translation: EAW58263.1. BC003520 mRNA. Translation: AAH03520.2. BC011959 mRNA. Translation: AAH11959.2. BC108686 mRNA. Translation: AAI08687.1. Different initiation. BC139834 mRNA. Translation: AAI39835.1. AK026854 mRNA. Translation: BAB15574.1. Different initiation. AK074532 mRNA. Translation: BAC11043.1. Different initiation.
IPI	IPI00013653. IPI00410264.
RefSeq	NP_077321.3. NM_024345.3.
UniGene	Hs.118394.
3D structure databases
ProteinModelPortal	Q5QP82.
SMR	Q5QP82. Positions 139-328, 525-557.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q5QP82. 3 interactions.
STRING	Q5QP82.
PTM databases
PhosphoSite	Q5QP82.
Polymorphism databases
DMDM	74762212.
Proteomic databases
PRIDE	Q5QP82.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000377724; ENSP00000366953; ENSG00000122741.
GeneID	79269.
KEGG	hsa:79269.
UCSC	uc004aao.1. human.
Organism-specific databases
CTD	79269.
GeneCards	GC09P037800.
HGNC	HGNC:23686. DCAF10.
HPA	HPA024160.
neXtProt	NX_Q5QP82.
PharmGKB	PA134967983. PA165585713.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG04554.
GeneTree	ENSGT00390000012666.
HOVERGEN	HBG055755.
InParanoid	Q5QP82.
OMA	DDEECTC.
OrthoDB	EOG4Z36DS.
PhylomeDB	Q5QP82.
Gene expression databases
ArrayExpress	Q5QP82.
Bgee	Q5QP82.
CleanEx	HS_WDR32.
Genevestigator	Q5QP82.
Family and domain databases
InterPro	IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR011046. WD40_repeat-like_dom. IPR019775. WD40_repeat_CS. IPR017986. WD40_repeat_dom. [Graphical view]
Gene3D	G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 2 hits.
KO	K11802.
Pfam	PF00400. WD40. 3 hits. [Graphical view]
SMART	SM00320. WD40. 5 hits. [Graphical view]
SUPFAM	SSF50978. WD40_like. 1 hit.
PROSITE	PS00678. WD_REPEATS_1. 1 hit. PS50082. WD_REPEATS_2. 2 hits. PS50294. WD_REPEATS_REGION. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	68226.

Entry information

Entry name

DCA10_HUMAN

Accession

Primary (citable) accession number: Q5QP82
Secondary accession number(s): A4VCJ5 , Q32NE2, Q8N2Q5, Q96ET5, Q9BTQ5, Q9H5P6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	January 4, 2005
Last modified:	January 25, 2012
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.