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Protein

DDB1- and CUL4-associated factor 10

Gene

DCAF10

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Biological processi

  • protein ubiquitination Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

SignaLinkiQ5QP82.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
DDB1- and CUL4-associated factor 10
Alternative name(s):
WD repeat-containing protein 32
Gene namesi
Name:DCAF10
Synonyms:WDR32
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

HGNCiHGNC:23686. DCAF10.

Subcellular locationi

GO - Cellular componenti

  • Cul4-RING E3 ubiquitin ligase complex Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000122741.
PharmGKBiPA165585713.

Polymorphism and mutation databases

DMDMi74762212.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003068331 – 559DDB1- and CUL4-associated factor 10Add BLAST559

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei53PhosphoserineBy similarity1
Modified residuei63PhosphoserineCombined sources1
Modified residuei89PhosphoserineBy similarity1
Modified residuei92PhosphoserineCombined sources1
Modified residuei134Omega-N-methylarginineCombined sources1
Modified residuei349PhosphoserineCombined sources1

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

EPDiQ5QP82.
MaxQBiQ5QP82.
PaxDbiQ5QP82.
PeptideAtlasiQ5QP82.
PRIDEiQ5QP82.

PTM databases

iPTMnetiQ5QP82.
PhosphoSitePlusiQ5QP82.

Expressioni

Gene expression databases

BgeeiENSG00000122741.
CleanExiHS_WDR32.
ExpressionAtlasiQ5QP82. baseline and differential.
GenevisibleiQ5QP82. HS.

Organism-specific databases

HPAiHPA024160.

Interactioni

Subunit structurei

Interacts with DDB1.1 Publication

Protein-protein interaction databases

BioGridi122612. 37 interactors.
IntActiQ5QP82. 10 interactors.
STRINGi9606.ENSP00000366953.

Structurei

3D structure databases

ProteinModelPortaliQ5QP82.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati166 – 205WD 1Add BLAST40
Repeati209 – 247WD 2Add BLAST39
Repeati251 – 290WD 3Add BLAST40
Repeati296 – 335WD 4Add BLAST40
Repeati408 – 448WD 5Add BLAST41
Repeati470 – 508WD 6Add BLAST39
Repeati526 – 559WD 7Add BLAST34

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi34 – 100Pro-richAdd BLAST67
Compositional biasi349 – 385Ser-richAdd BLAST37

Sequence similaritiesi

Belongs to the WD repeat DCAF10 family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiENOG410ISIZ. Eukaryota.
ENOG410ZF1A. LUCA.
GeneTreeiENSGT00390000012666.
HOGENOMiHOG000006516.
HOVERGENiHBG055755.
InParanoidiQ5QP82.
KOiK11802.
OMAiHGNCITS.
OrthoDBiEOG091G09PD.
PhylomeDBiQ5QP82.
TreeFamiTF323434.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 2 hits.
[Graphical view]
SMARTiSM00320. WD40. 5 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5QP82-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFPFGPHSPG GDGSAGAGAE EPTPHEGQAA ATGPPSPLHP GADATHPPPP
60 70 80 90 100
ARSPRRPGAP SLSPAPRSGE LGLPGAPESS TASAPGEPSP PSPPCRRPGP
110 120 130 140 150
DCRAKSRGRH GLGAGLGGPG ARLFGWLKER SLGRGLFVDP ARDNFRTMTS
160 170 180 190 200
LYGSIHPADS VYLSTRTHGA VFNLEYSPDG SVLTVACEQT EVLLFDPISS
210 220 230 240 250
KHIKTLSEAH EDCVNNIRFL DNRLFATCSD DTTIALWDLR KLNTKVCTLH
260 270 280 290 300
GHTSWVKNIE YDTNTRLLVT SGFDGNVIIW DTNRYTEDGC PHKKFFHTRF
310 320 330 340 350
LMRMRLTPDC SKMLISTSSG YLLILHDLDL TKSLEVGSYP ILRARRTTSS
360 370 380 390 400
SDLTTSSSSS GPRVSGSPCH HSDSNSSEKH MSRASQREGV SPRNSLEVVT
410 420 430 440 450
PEVLGESDHG NCITSLQLHP KGWATLLRCS SNSDDEECTC VYEFQEGAPV
460 470 480 490 500
RPVSPRCSLR LTHYIEEANV GRGYIKELCF SPDGRMISSP HGYGIRLLGF
510 520 530 540 550
DKQCSELVDC LPKEASPLRV IRSLYSHNDV VLTTKFSPTH CQIASGCLSG

RVSLYQPKF
Length:559
Mass (Da):60,582
Last modified:January 4, 2005 - v1
Checksum:i583B5A781ADAED38
GO
Isoform 2 (identifier: Q5QP82-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     352-388: Missing.

Note: No experimental confirmation available.
Show »
Length:522
Mass (Da):56,683
Checksum:i75DCBB1C9E20B368
GO

Sequence cautioni

The sequence AAI08687 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAB15574 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAC11043 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti309D → G in BAC11043 (PubMed:14702039).Curated1
Sequence conflicti332K → N in AAH03520 (PubMed:15489334).Curated1
Sequence conflicti457C → W in BAB15574 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_028513352 – 388Missing in isoform 2. 1 PublicationAdd BLAST37

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL138752 Genomic DNA. Translation: CAI13878.1.
CH471071 Genomic DNA. Translation: EAW58263.1.
BC003520 mRNA. Translation: AAH03520.2.
BC011959 mRNA. Translation: AAH11959.2.
BC108686 mRNA. Translation: AAI08687.1. Different initiation.
BC139834 mRNA. Translation: AAI39835.1.
AK026854 mRNA. Translation: BAB15574.1. Different initiation.
AK074532 mRNA. Translation: BAC11043.1. Different initiation.
CCDSiCCDS6613.2. [Q5QP82-1]
RefSeqiNP_001273739.1. NM_001286810.1.
NP_077321.3. NM_024345.4. [Q5QP82-1]
XP_005251634.1. XM_005251577.3. [Q5QP82-2]
UniGeneiHs.118394.

Genome annotation databases

EnsembliENST00000377724; ENSP00000366953; ENSG00000122741. [Q5QP82-1]
GeneIDi79269.
KEGGihsa:79269.
UCSCiuc004aao.5. human. [Q5QP82-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL138752 Genomic DNA. Translation: CAI13878.1.
CH471071 Genomic DNA. Translation: EAW58263.1.
BC003520 mRNA. Translation: AAH03520.2.
BC011959 mRNA. Translation: AAH11959.2.
BC108686 mRNA. Translation: AAI08687.1. Different initiation.
BC139834 mRNA. Translation: AAI39835.1.
AK026854 mRNA. Translation: BAB15574.1. Different initiation.
AK074532 mRNA. Translation: BAC11043.1. Different initiation.
CCDSiCCDS6613.2. [Q5QP82-1]
RefSeqiNP_001273739.1. NM_001286810.1.
NP_077321.3. NM_024345.4. [Q5QP82-1]
XP_005251634.1. XM_005251577.3. [Q5QP82-2]
UniGeneiHs.118394.

3D structure databases

ProteinModelPortaliQ5QP82.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122612. 37 interactors.
IntActiQ5QP82. 10 interactors.
STRINGi9606.ENSP00000366953.

PTM databases

iPTMnetiQ5QP82.
PhosphoSitePlusiQ5QP82.

Polymorphism and mutation databases

DMDMi74762212.

Proteomic databases

EPDiQ5QP82.
MaxQBiQ5QP82.
PaxDbiQ5QP82.
PeptideAtlasiQ5QP82.
PRIDEiQ5QP82.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000377724; ENSP00000366953; ENSG00000122741. [Q5QP82-1]
GeneIDi79269.
KEGGihsa:79269.
UCSCiuc004aao.5. human. [Q5QP82-1]

Organism-specific databases

CTDi79269.
GeneCardsiDCAF10.
HGNCiHGNC:23686. DCAF10.
HPAiHPA024160.
neXtProtiNX_Q5QP82.
OpenTargetsiENSG00000122741.
PharmGKBiPA165585713.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410ISIZ. Eukaryota.
ENOG410ZF1A. LUCA.
GeneTreeiENSGT00390000012666.
HOGENOMiHOG000006516.
HOVERGENiHBG055755.
InParanoidiQ5QP82.
KOiK11802.
OMAiHGNCITS.
OrthoDBiEOG091G09PD.
PhylomeDBiQ5QP82.
TreeFamiTF323434.

Enzyme and pathway databases

UniPathwayiUPA00143.
SignaLinkiQ5QP82.

Miscellaneous databases

ChiTaRSiDCAF10. human.
GenomeRNAii79269.
PROiQ5QP82.

Gene expression databases

BgeeiENSG00000122741.
CleanExiHS_WDR32.
ExpressionAtlasiQ5QP82. baseline and differential.
GenevisibleiQ5QP82. HS.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 2 hits.
[Graphical view]
SMARTiSM00320. WD40. 5 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDCA10_HUMAN
AccessioniPrimary (citable) accession number: Q5QP82
Secondary accession number(s): A4VCJ5
, Q32NE2, Q8N2Q5, Q96ET5, Q9BTQ5, Q9H5P6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: January 4, 2005
Last modified: November 2, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.