Reviewed, UniProtKB/Swiss-Prot Q5QN75 (M2K1_ORYSJ)

Names and origin

Protein names

Recommended name:
    Mitogen-activated protein kinase kinase 1
      Short name=MAP kinase kinase 1
      Short name=MAPKK1
    EC=2.7.12.2
Alternative name(s):
    OsMEK1

Gene names

Name:	MEK1
Ordered Locus Names:	Os01g0510100, LOC_Os01g32660
ORF Names:	P0455H03.45, P0520B06.8

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	355 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Activated through serine and threonine phosphorylation By similarity.
Subunit structure	Interacts with MPK5/MAPK5. Ref.1
Tissue specificity	Expressed in roots, leaf sheaths and panicles. Ref.1
Induction	By low temperature (12 degrees Celsius) treatment. Ref.1
Post-translational modification	Phosphorylated on Ser-221 and Thr-227, which activates the enzyme By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 355	355	Mitogen-activated protein kinase kinase 1		PRO_0000239761
Regions
	Domain	71 – 330	260	Protein kinase
	Nucleotide binding	77 – 85	9	ATP By similarity
Sites
	Active site	193	1	Proton acceptor By similarity
	Binding site	100	1	ATP By similarity
Amino acid modifications
	Modified residue	221	1	Phosphoserine By similarity
	Modified residue	227	1	Phosphothreonine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5QN75-1 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: B579E8B7DCB861F4	FASTA	355	40,000
	10 20 30 40 50 60 MRGKKPHKEL KLSVPAQETP VDKFLTASGT FKDGELRLNQ RGLQLISEET ADEPQSTNLK 70 80 90 100 110 120 VEDVQLSMDD LEMIQVIGKG SGGIVQLVRH KWVGTLYALK GIQMNIQEAV RKQIVQELKI 130 140 150 160 170 180 NQATQNAHIV LCHQSFYHNG VIYLVLEYMD RGSLADIIKQ VKTILEPYLA VLCKQVLEGL 190 200 210 220 230 240 LYLHHERHVI HRDIKPSNLL VNRKGEVKIT DFGVSAVLAS SMGQRDTFVG TYNYMAPERI 250 260 270 280 290 300 SGSSYDYKSD IWSLGLVILE CAIGRFPYIP SEGEGWLSFY ELLEAIVDQP PPSAPADQFS 310 320 330 340 350 PEFCAFISSC IQKDPAERMS ASELLNHPFI KKFEDKDLDL RILVESLEPP MNISE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two novel mitogen-activated protein signaling components, OsMEK1 and OsMAP1, are involved in a moderate low-temperature signaling pathway in rice." Wen J.-Q., Oono K., Imai R. Plant Physiol. 129:1880-1891(2002) [PubMed: 12177502] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH MPK5/MAPK5, TISSUE SPECIFICITY, INDUCTION.
[2]	"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed: 12447438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF216314 mRNA. Translation: AAG40578.1. AP003077 Genomic DNA. Translation: BAD73135.1. AP003435 Genomic DNA. Translation: BAD73553.1.
RefSeq	NP_001043164.1.
UniGene	Os.2619
3D structure databases
SMR	Q5QN75. Positions 63-341.
ModBase	Search...
Genome annotation databases
GeneID	4324023.
KEGG	osa:4324023.
Organism-specific databases
Gramene	Q5QN75.
Phylogenomic databases
PhylomeDB	Q5QN75.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR002290. Ser/Thr_prot_kinase_dom. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

M2K1_ORYSJ

Accession

Primary (citable) accession number: Q5QN75
Secondary accession number(s): Q9FQM4

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 13, 2006
Last sequence update:	January 4, 2005
Last modified:	January 19, 2010
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)