Reviewed, UniProtKB/Swiss-Prot Q5QMN3 (RH20_ORYSJ)

Names and origin

Protein names

Recommended name:
    DEAD-box ATP-dependent RNA helicase 20
    EC=3.6.1.-

Gene names

Ordered Locus Names:	Os01g0197200, LOC_Os01g10050
ORF Names:	P0419B01.12

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	494 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	ATP-dependent RNA helicase involved nonsense-mediated mRNA decay and ribosome biogenesis through rRNA processing By similarity.
Subcellular location	Nucleus By similarity.
Domain	The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.
Sequence similarities	Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.
Sequence caution	The sequence BAD73320.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence BAF04212.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Nonsense-mediated mRNA decay Ribosome biogenesis rRNA processing
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Helicase Hydrolase
Gene Ontology (GO)
Biological process	nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Inferred from electronic annotation. Source: UniProtKB-KW rRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent helicase activity Inferred from electronic annotation. Source: InterPro RNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 494	494	DEAD-box ATP-dependent RNA helicase 20		PRO_0000282455
Regions
	Domain	122 – 297	176	Helicase ATP-binding
	Domain	325 – 470	146	Helicase C-terminal
	Nucleotide binding	135 – 142	8	ATP By similarity
	Motif	91 – 119	29	Q motif
	Motif	245 – 248	4	DEAD box

Sequences

	Sequence		Length	Mass (Da)
	Q5QMN3-1 [UniParc]. Last modified April 3, 2007. Version 2. Checksum: 6C02BF94824EB3CD	FASTA	494	54,674
	10 20 30 40 50 60 MSRFDGRAAD PGSYRDRRSE GAFGGGTRAF APTSKADSAA AAADLDGLPR FEKNFYVESP 70 80 90 100 110 120 SVAGMTEEEV EAYRRRREIT VEGRDVPKPV REFRDVGFPE YVLQEITKAG FVEPTPIQSQ 130 140 150 160 170 180 GWPMALRGRD LIGIAETGSG KTLAYLLPAI VHVNAQPILA PGDGPIVLVL APTRELAVQI 190 200 210 220 230 240 QQEATKFGAS SKIKSTCIYG GVPKGPQVRD LQKGVEIVIA TPGRLIDMIE SHHTNLRRVT 250 260 270 280 290 300 YLVLDEADRM LDMGFEPQIK KIVSQIRPDR QTLYWSATWP KEVEQLARNF LFDPYKVIIG 310 320 330 340 350 360 SEELKANHAI SQHVEILSES QKYNKLVNLL EDIMDGSRIL IFMDTKKGCD QITRQLRMDG 370 380 390 400 410 420 WPALSIHGDK SQAERDWVLS EFKSGKSPIM TATDVAARGL DVKDVKYVIN YDFPGSLEDY 430 440 450 460 470 480 VHRIGRTGRA GAKGTAYTFF TAANARFAKD LINILEEAGQ KVSPELANMG RGAPPPSSGH 490 RDRYRGYGGG RSWS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed: 12447438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana." The rice annotation project (RAP) Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed: 15685292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP003244 Genomic DNA. Translation: BAD73320.1. Sequence problems. AP008207 Genomic DNA. Translation: BAF04212.1. Sequence problems. CM000138 Genomic DNA. No translation available.
RefSeq	NP_001042298.1.
UniGene	Os.71079
3D structure databases
HSSP	HSSP built from PDB template 2DB3 based on UniProtKB P09052.
ModBase	Search...
Genome annotation databases
GeneID	4327330.
KEGG	osa:4327330.
NMPDR	fig|39947.1.peg.5208.
Organism-specific databases
Gramene	Q5QMN3.
Family and domain databases
InterPro	IPR014001. DEAD-like_N. IPR001650. DNA/RNA_helicase_C. IPR011545. DNA/RNA_helicase_DEAD/DEAH_N. IPR014021. Helicase_SF1/SF2_ATP-bd. IPR000629. RNA-helicase_DEAD-box_CS. IPR014014. RNA_helicase_DEAD_Q_motif. [Graphical view]
Pfam	PF00270. DEAD. 1 hit. PF00271. Helicase_C. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
PROSITE	PS00039. DEAD_ATP_HELICASE. 1 hit. PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. PS51195. Q_MOTIF. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RH20_ORYSJ

Accession

Primary (citable) accession number: Q5QMN3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2007
Last sequence update:	April 3, 2007
Last modified:	November 24, 2009
This is version 44 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)