Q5QJV7 (ERR1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Steroid hormone receptor ERR1

Alternative name(s):
Estrogen-related receptor alpha
Short name=ERR-alpha
Nuclear receptor subfamily 3 group B member 1

Gene names

Name:	Esrra
Synonyms:	Nr3b1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	422 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Binds to an ERR-alpha response element (ERRE) containing a single consensus half-site, 5'-TNAAGGTCA-3'. Can bind to the medium-chain acyl coenzyme A dehydrogenase (MCAD) response element NRRE-1 and may act as an important regulator of MCAD promoter. Binds to the C1 region of the lactoferrin gene promoter. Requires dimerization and the coactivator, PGC-1A, for full activity. The ERRalpha/PGC1alpha complex is a regulator of energy metabolism.
Subunit structure	Binds DNA as a monomer or a homodimer. Interacts (via the AF2 domain) with coactivator PPARGC1A (via the L3 motif); the interaction greatly enhances transriptional activity of genes involved in energy metabolism. Interacts with PIAS4; the interaction enhances sumoylation By similarity.
Subcellular location	Nucleus By similarity.
Post-translational modification	Phosphorylation on Ser-19 enhances sumoylation on Lys-14 increasing repression of transcriptional activity By similarity. Sumoylated with SUMO2. Main site is Lys-14 which is enhanced by phosphorylation on Ser-19, cofactor activation, and by interaction with PIAS4. Sumoylation enhances repression of transcriptional activiy, but has no effect on subcellular location nor on DNA binding By similarity.
Sequence similarities	Belongs to the nuclear hormone receptor family. NR3 subfamily. Contains 1 nuclear receptor DNA-binding domain.
Sequence caution	The sequence AAI04702.1 differs from that shown. Reason: Erroneous initiation. The sequence AAQ17212.1 differs from that shown. Reason: Frameshift at positions 408 and 413.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Receptor
PTM	Isopeptide bond Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cartilage development Inferred from mutant phenotype PubMed 17170100. Source: RGD positive regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara positive regulation of transcription, DNA-dependent Inferred from mutant phenotype PubMed 17170100. Source: RGD regulation of cell proliferation Inferred from mutant phenotype PubMed 11381083. Source: RGD regulation of osteoblast differentiation Inferred from mutant phenotype PubMed 11381083. Source: RGD regulation of osteoclast differentiation Inferred from mutant phenotype PubMed 12193582. Source: RGD transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: Compara steroid binding Inferred from electronic annotation. Source: InterPro steroid hormone receptor activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 422	422	Steroid hormone receptor ERR1		PRO_0000295232
Regions
	DNA binding	76 – 151	76	Nuclear receptor
	Zinc finger	79 – 99	21	NR C4-type
	Zinc finger	115 – 134	20	NR C4-type
	Region	1 – 76	76	Repressor domain By similarity
	Region	205 – 401	197	Ligand binding domain By similarity
	Region	402 – 422	21	AF-2 domain By similarity
Sites
	Site	124	1	Required for DNA-dependent dimerization By similarity
Amino acid modifications
	Modified residue	19	1	Phosphoserine By similarity
	Modified residue	22	1	Phosphoserine By similarity
	Cross-link	14		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity
	Cross-link	402		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5QJV7 [UniParc]. Last modified July 10, 2007. Version 2. Checksum: 2F54EEA1BD3B511D	FASTA	422	45,464
	10 20 30 40 50 60 MSSQVVGIEP LYIKAEPASP DSPKGSSETE TEPPVTLASG PAPARCLPGH KEEEDGEGAG 70 80 90 100 110 120 SGEQGSGKLV LSSLPKRLCL VCGDVASGYH YGVASCEACK AFFKRTIQGS IEYSCPASNE 130 140 150 160 170 180 CEITKRRRKA CQACRFTKCL RVGMLKEGVR LDRVRGGRQK YKRRPEVDPL PFPGPFPAGP 190 200 210 220 230 240 LAVAGGPRKT APVNALVSHL LVVEPEKLYA MPDPASPDGH LPAVATLCDL FDREIVVTIS 250 260 270 280 290 300 WAKSIPGFSS LSLSDQMSVL QSVWMEVLVL GVAQRSLPLQ DELAFAEDLV LDEEGARAAG 310 320 330 340 350 360 LGDLGAALLQ LVRRLQALRL EREEYVLLKA LALANSDSVH IEDAEAVEQL REALHEALLE 370 380 390 400 410 420 YEAGRAGPGG GAERRRAGRL LLTLPLLRQT AGKVLAHFYG VKLEGKVPMH KLFLEMLEAM MD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and expression study of estrogen receptor-related receptor alpha in rat ovary." Lui K., Chen S., Chan F.L. Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Heart.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY280663 mRNA. Translation: AAQ17212.1. Frameshift. BC104701 mRNA. Translation: AAI04702.1. Different initiation.
IPI	IPI00371457.
RefSeq	NP_001008511.2. NM_001008511.2.
UniGene	Rn.130171.
3D structure databases
HSSP	HSSP built from PDB template 1XB7 based on UniProtKB P11474.
ProteinModelPortal	Q5QJV7.
SMR	Q5QJV7. Positions 73-421.
ModBase	Search...
PTM databases
PhosphoSite	Q5QJV7.
Proteomic databases
PaxDb	Q5QJV7.
PRIDE	Q5QJV7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000028697; ENSRNOP00000028697; ENSRNOG00000021139.
GeneID	293701.
KEGG	rno:293701.
UCSC	RGD:1583866. rat.
Organism-specific databases
CTD	2101.
RGD	1583866. Esrra.
Phylogenomic databases
eggNOG	NOG282629.
GeneTree	ENSGT00700000104391.
HOGENOM	HOG000233467.
HOVERGEN	HBG108344.
InParanoid	Q5QJV7.
KO	K08552.
OMA	PLPFPGS.
OrthoDB	EOG48KRBN.
Gene expression databases
Genevestigator	Q5QJV7.
Family and domain databases
Gene3D	1.10.565.10. 1 hit. 3.30.50.10. 1 hit.
InterPro	IPR008946. Nucl_hormone_rcpt_ligand-bd. IPR000536. Nucl_hrmn_rcpt_lig-bd_core. IPR027289. Oest-rel_rcp. IPR024178. Oest_rcpt/oest-rel_rcp. IPR001723. Str_hrmn_rcpt. IPR001628. Znf_hrmn_rcpt. IPR013088. Znf_NHR/GATA. [Graphical view]
Pfam	PF00104. Hormone_recep. 1 hit. PF00105. zf-C4. 1 hit. [Graphical view]
PIRSF	PIRSF002527. ER-like_NR. 1 hit. PIRSF500939. ERR1-2-3. 1 hit.
PRINTS	PR00398. STRDHORMONER. PR00047. STROIDFINGER.
SMART	SM00430. HOLI. 1 hit. SM00399. ZnF_C4. 1 hit. [Graphical view]
SUPFAM	SSF48508. Str_ncl_receptor. 1 hit.
PROSITE	PS00031. NUCLEAR_REC_DBD_1. 1 hit. PS51030. NUCLEAR_REC_DBD_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	636801.

Entry information

Entry name

ERR1_RAT

Accession

Primary (citable) accession number: Q5QJV7
Secondary accession number(s): Q3MHT1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	July 10, 2007
Last modified:	April 3, 2013
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program