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Protein

Bifunctional nitrilase/nitrile hydratase NIT4A

Gene

NIT4A

Organism
Lupinus angustifolius (Narrow-leaved blue lupine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the cyanide detoxification pathway. Has nitrilase and nitrile-hydratase activity in the ratio 4.0:1, producing both asparagine and aspartic acid from beta-cyano-L-alanine (Ala(CN)). Can also use 3-phenylpropionitrile as substrate, but not indole-3-acetonitrile.1 Publication

Catalytic activityi

L-asparagine = 3-cyanoalanine + H2O.1 Publication
3-cyano-L-alanine + 2 H2O = L-aspartate + NH3.1 Publication

Kineticsi

  1. KM=1.18 mM for Ala(CN)1 Publication
  1. Vmax=477.7 nmol/sec/mg enzyme for the nitrilase activity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei69Proton acceptorPROSITE-ProRule annotation1
Active sitei156PROSITE-ProRule annotation1
Active sitei190NucleophilePROSITE-ProRule annotation1

GO - Molecular functioni

  • 3-cyanoalanine hydratase activity Source: UniProtKB
  • cyanoalanine nitrilase activity Source: UniProtKB

GO - Biological processi

  • cyanide catabolic process Source: UniProtKB
  • detoxification of nitrogen compound Source: UniProtKB

Keywordsi

Molecular functionHydrolase, Lyase

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-17621.
BRENDAi3.5.5.4. 3090.
4.2.1.65. 3090.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional nitrilase/nitrile hydratase NIT4A (EC:3.5.5.4, EC:4.2.1.65)
Short name:
LaNIT4A
Alternative name(s):
3-cyanoalanine hydratase
Cyanoalanine nitrilase A
Gene namesi
Name:NIT4A
OrganismiLupinus angustifolius (Narrow-leaved blue lupine)
Taxonomic identifieri3871 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeGenisteaeLupinus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004301461 – 349Bifunctional nitrilase/nitrile hydratase NIT4AAdd BLAST349

Proteomic databases

PRIDEiQ5QGZ8.

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ5QGZ8.
SMRiQ5QGZ8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini29 – 301CN hydrolasePROSITE-ProRule annotationAdd BLAST273

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi249 – 252Poly-Pro4

Sequence similaritiesi

Phylogenomic databases

KOiK13035.

Family and domain databases

Gene3Di3.60.110.10. 1 hit.
InterProiView protein in InterPro
IPR003010. C-N_Hydrolase.
IPR036526. C-N_Hydrolase_sf.
IPR000132. Nitrilase/CN_hydratase_CS.
PfamiView protein in Pfam
PF00795. CN_hydrolase. 1 hit.
SUPFAMiSSF56317. SSF56317. 1 hit.
PROSITEiView protein in PROSITE
PS50263. CN_HYDROLASE. 1 hit.
PS00920. NITRIL_CHT_1. 1 hit.
PS00921. NITRIL_CHT_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5QGZ8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALVTTPTVN DGPLFAEVNM SSDFNAPTVR ATVVQASTIF YDTPATLDKA
60 70 80 90 100
ERLLAEAASY GAQIVVFPEA FIGGYPRGSN FGVSIGNRTA KGKEDFRKYH
110 120 130 140 150
SAAIDVPGPE VDRLAALAGK YKVYLVMGVI ERDGYTLYCT VLFFGAQGRY
160 170 180 190 200
LGKHRKLMPT ALERIIWGFG DGSTIPVFET PIGKIGAAIC WENKMPLLRT
210 220 230 240 250
AMYAKGVEIY CAPTADSREV WQASMTHIAL EGGCFVLSAN QFCRRRDYPP
260 270 280 290 300
PPEYVFEGTE ENLTPDSVVC AGGSVIISPS GAVLAGPSYE GEALISADLD
310 320 330 340
LGEIARAKFD FDVVGHYSRP EVLSLVVKDH PTNPVTFTSA STKIEDKTK
Length:349
Mass (Da):37,762
Last modified:January 4, 2005 - v1
Checksum:iBEC5FEFC46D52068
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY547301 mRNA. Translation: AAT36331.1.
DQ241759 Genomic DNA. Translation: ABB51979.1.
AY547303 Genomic DNA. Translation: AAS55944.1.
RefSeqiXP_019448859.1. XM_019593314.1.

Genome annotation databases

GeneIDi109351736.
KEGGilang:109351736.

Similar proteinsi

Entry informationi

Entry nameiNRL4A_LUPAN
AccessioniPrimary (citable) accession number: Q5QGZ8
Secondary accession number(s): Q6QDB7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 3, 2014
Last sequence update: January 4, 2005
Last modified: October 25, 2017
This is version 45 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families