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Q5QGZ2 (NSP4_ROTGA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 24. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Non-structural glycoprotein 4

Short name=NSP4
OrganismRotavirus B (isolate Human/China/ADRV/1982) (RV-B) (Rotavirus B (isolate adult diarrhea rotavirus))
Taxonomic identifier10942 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirus
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length219 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in virus morphogenesis. Functions as a receptor for the immature double-layered inner capsid particle (ICP) which transiently buds into the lumen of the rough endoplasmic reticulum during viral maturation By similarity.

Enterotoxin that causes a phospholipase C-dependent elevation of the intracellular calcium concentration in host intestinal mucosa cells. Increased concentration of intracellular calcium disrupts the cytoskeleton and the tight junctions, raising the paracellular permeability. Potentiates chloride ion secretion through a calcium ion-dependent signaling pathway, inducing age-dependent diarrhea. To perform this enterotoxigenic role in vivo, NSP4 is probably released from infected enterocytes in a soluble form capable of diffusing within the intestinal lumen and interacting with the plasma membrane receptors on neighboring epithelial cells By similarity.

Subunit structure

Homotetramer By similarity.

Subcellular location

Non-structural glycoprotein 4: Host rough endoplasmic reticulum membrane; Single-pass type III membrane protein By similarity. Host membranehost caveola; Single-pass type III membrane protein. Secreted By similarity. Note: Immature double-layered particles assembled in the cytoplasm bud across the membrane of the endoplasmic reticulum, acquiring during this process a transient lipid membrane that is modified with the ER resident viral glycoproteins NSP4 and VP7; these enveloped particles also contain VP4. As the particles move towards the interior of the ER cisternae, the transient lipid membrane and the non-structural protein NSP4 are lost, while the virus surface proteins VP4 and VP7 rearrange to form the outermost virus protein layer, yielding mature infectious triple-layered particles. NSP4 also localizes in vesicular structures, which contain an autophagosomal marker and associate with viroplasms in virus-infected cells By similarity.

Domain

The coiled coil region mediates oligomerization By similarity.

Sequence similarities

Belongs to the rotavirus NSP4 family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 219219Non-structural glycoprotein 4
PRO_0000369864

Regions

Topological domain1 – 4242Lumenal By similarity
Transmembrane43 – 6018Helical; Potential
Topological domain61 – 219159Cytoplasmic By similarity
Compositional bias36 – 394Poly-Ser

Sequences

Sequence LengthMass (Da)Tools
Q5QGZ2 [UniParc].

Last modified January 4, 2005. Version 1.
Checksum: 9569C7E9763005BD

FASTA21925,347
        10         20         30         40         50         60 
MAESSDMQEL FVQATYEEIL KLASSVNHEQ IRETISSSSP QKILTGTMLT LGALLTTLIV 

        70         80         90        100        110        120 
KKKGTKFLTT KVQSNVAYLA EMVVWKANQT VKTICDEVLH QRKVIEKLQC LDKMCSEIEK 

       130        140        150        160        170        180 
LRYDMEHFQG MDVTKELIQM CEQKMIDIDS KVKEVERSCD RRIRDYDWKI ATLTAHPTQQ 

       190        200        210 
LPAHIDIINQ HIEDDAETQI IQQHMNKQAR VKLNSRNRL 

« Hide

References

[1]"Molecular characterization of the rotavirus NSP4 enterotoxin homologue from group B rotavirus."
Guzman E., McCrae M.A.
Virus Res. 110:151-160(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY548957 Genomic RNA. Translation: AAT36306.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameNSP4_ROTGA
AccessionPrimary (citable) accession number: Q5QGZ2
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: January 4, 2005
Last modified: February 19, 2014
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families