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Protein

A-kinase anchor protein 12

Gene

Akap12

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC).By similarity

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
A-kinase anchor protein 12
Short name:
AKAP-12
Gene namesi
Name:Akap12
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi70988. Akap12.

Subcellular locationi

  • Cytoplasmcytoskeleton By similarity
  • Membrane By similarity; Lipid-anchor By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 16871686A-kinase anchor protein 12PRO_0000304942Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineBy similarity
Modified residuei11 – 111PhosphoserineCombined sources
Modified residuei18 – 181PhosphoserineBy similarity
Modified residuei22 – 221PhosphoserineBy similarity
Modified residuei27 – 271PhosphoserineBy similarity
Modified residuei136 – 1361PhosphoserineBy similarity
Modified residuei204 – 2041PhosphoserineBy similarity
Modified residuei235 – 2351PhosphoserineBy similarity
Modified residuei245 – 2451PhosphoserineCombined sources
Modified residuei268 – 2681PhosphoserineCombined sources
Modified residuei271 – 2711PhosphoserineCombined sources
Modified residuei274 – 2741PhosphoserineCombined sources
Modified residuei304 – 3041PhosphoserineCombined sources
Modified residuei331 – 3311PhosphothreonineCombined sources
Modified residuei335 – 3351PhosphoserineCombined sources
Modified residuei350 – 3501PhosphoserineCombined sources
Modified residuei353 – 3531PhosphotyrosineBy similarity
Modified residuei371 – 3711PhosphoserineBy similarity
Modified residuei469 – 4691PhosphoserineCombined sources
Modified residuei491 – 4911PhosphoserineCombined sources
Modified residuei507 – 5071PhosphoserineCombined sources
Modified residuei509 – 5091PhosphoserineCombined sources
Modified residuei541 – 5411PhosphoserineCombined sources
Modified residuei544 – 5441PhosphoserineCombined sources
Modified residuei585 – 5851PhosphoserineCombined sources
Modified residuei599 – 5991PhosphoserineCombined sources
Modified residuei614 – 6141PhosphoserineCombined sources
Modified residuei616 – 6161PhosphoserineBy similarity
Modified residuei629 – 6291PhosphothreonineCombined sources
Modified residuei631 – 6311PhosphoserineCombined sources
Modified residuei632 – 6321PhosphoserineBy similarity
Modified residuei635 – 6351PhosphoserineCombined sources
Modified residuei638 – 6381PhosphoserineCombined sources
Modified residuei683 – 6831PhosphoserineCombined sources
Modified residuei684 – 6841PhosphoserineBy similarity
Modified residuei685 – 6851PhosphoserineBy similarity
Modified residuei736 – 7361PhosphoserineCombined sources
Modified residuei748 – 7481PhosphoserineBy similarity
Modified residuei770 – 7701PhosphoserineCombined sources
Modified residuei771 – 7711PhosphoserineCombined sources
Modified residuei789 – 7891PhosphoserineBy similarity
Modified residuei871 – 8711PhosphothreonineBy similarity
Modified residuei873 – 8731PhosphoserineBy similarity
Cross-linki1030 – 1030Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Modified residuei1059 – 10591PhosphoserineCombined sources
Modified residuei1289 – 12891PhosphoserineBy similarity
Modified residuei1348 – 13481PhosphoserineCombined sources
Modified residuei1352 – 13521PhosphoserineCombined sources
Modified residuei1354 – 13541PhosphoserineCombined sources
Modified residuei1543 – 15431PhosphoserineBy similarity
Modified residuei1571 – 15711PhosphoserineCombined sources
Modified residuei1637 – 16371PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated by PKC (in vitro).1 Publication

Keywords - PTMi

Isopeptide bond, Lipoprotein, Myristate, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ5QD51.
PRIDEiQ5QD51.

PTM databases

iPTMnetiQ5QD51.
PhosphoSiteiQ5QD51.

Expressioni

Gene expression databases

BgeeiENSRNOG00000019549.
ExpressionAtlasiQ5QD51. baseline and differential.
GenevisibleiQ5QD51. RN.

Interactioni

Subunit structurei

Binds to dimeric RII-alpha regulatory subunit of PKC.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000057494.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini591 – 62131AKAP 1Add
BLAST
Domaini740 – 77031AKAP 2Add
BLAST
Domaini781 – 81131AKAP 3Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni254 – 544291Involved in PKC-bindingBy similarityAdd
BLAST
Regioni1498 – 151114RII-bindingBy similarityAdd
BLAST

Sequence similaritiesi

Contains 3 AKAP domains.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IFC9. Eukaryota.
ENOG4110Y3C. LUCA.
GeneTreeiENSGT00730000111244.
HOGENOMiHOG000293190.
HOVERGENiHBG050472.
InParanoidiQ5QD51.
KOiK16528.
OMAiFARTETA.
OrthoDBiEOG091G01X9.
PhylomeDBiQ5QD51.
TreeFamiTF105411.

Family and domain databases

InterProiIPR028540. AKAP12.
IPR001573. Pkinase-A_anch_WSK-motif.
IPR018459. RII_binding_1.
[Graphical view]
PANTHERiPTHR23209. PTHR23209. 1 hit.
PfamiPF10522. RII_binding_1. 1 hit.
PF03832. WSK. 3 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5QD51-1) [UniParc]FASTAAdd to basket
Also known as: Alpha

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGAGSSTEQR SPEQPAGSDT PSELVLSGHG PAAEASGAAG DPADADPATK
60 70 80 90 100
LPQKNGQLST VNGVAEQGDV HVQEENQEGQ EEEVVDEDVG QRESEDVREK
110 120 130 140 150
DRVEEMAANS TAVEDITKDG QEETSEIIEQ IPASESNVEE MVQPAESQAN
160 170 180 190 200
DVGFKKVFKF VGFKFTVKKD KNEKSDTVQL LTVKKDEGEG AEASVGAGDH
210 220 230 240 250
QEPSVETAVG ESASKESELK QSTEKQEGTL KQEQSSTEIP LQAESDQAAE
260 270 280 290 300
EEAKDEGEEK QEKEPTKSPE SPSSPVSSET TSSFKKFFTH GWAGWRKKTS
310 320 330 340 350
FKKSKEDDLE TAEKRKEQEA EKVDEEEKEK TEPASEEQEP AEDTDQARLS
360 370 380 390 400
ADYEKVELPL EDQVGDLEAS SEEKCAPLAT EVFDEKMEAH QEVVAEVHVS
410 420 430 440 450
TVEKTEEEQG GGGEAEGGVV VEGTGESLPP EKLAEPQEVP QEAEPAEELM
460 470 480 490 500
KSREMCVSGG DHTQLTDLSP EEKTLPKHPE GIVSEVEMLS SQERIKVQGS
510 520 530 540 550
PLKKLFSSSG LKKLSGKKQK GKRGGGGDEE PGEYQHIHTE SPESADEQKG
560 570 580 590 600
ESSASSPEEP EETTCLEKGP LEAPQDGEAE EGTTSDGEKK REGITPWASF
610 620 630 640 650
KKMVTPKKRV RRPSESDKEE ELEKVKSATL SSTDSTVSEM QDEVKTVGEE
660 670 680 690 700
QKPEEPKRRV DTSVSWEALI CVGSSKKRAR KASSSDDEGG PRTLGGDSHR
710 720 730 740 750
AEEASKDKEA GTDAVPASTQ EQDQAQGSSS PEPAGSPSEG EGVSTWESFK
760 770 780 790 800
RLVTPRKKSK SKLEEKAEDS SVEQLSTEIE PSREESWVSI KKFIPGRRKK
810 820 830 840 850
RADGKQEQAT VEDSGPVEIN EDDPDVPAVV PLSEYDAVER EKMEAQGNAE
860 870 880 890 900
LPQLLGAVYV SEELSKTLVH TVSVAVIDGT RAVTSVEERS PSWISASVTE
910 920 930 940 950
PLEHTAGEAM PPVEEVTEKD IIAEETPVLT QTLPEGKDAH DDMVTSEVDF
960 970 980 990 1000
TSEAVTATET SEALRTEEVT EASGAEETTD MVSAVSQLTD SPDTTEEATP
1010 1020 1030 1040 1050
VQEVEGGVLD TEEEERQTQA ILQAVADKVK EESQVPATQT VQRTGSKALE
1060 1070 1080 1090 1100
KVEEVEEDSE VLASEKEKDV MPKGPVQEAG AEHLAQGSET GQATPESLEV
1110 1120 1130 1140 1150
PEVTADVDHV ATCQVIKLQQ LMEQAVAPES SETLTDSETN GSTPLADSDT
1160 1170 1180 1190 1200
ADGTQQDETI DSQDSKATAA VRQSQVTEEE AATAQKEEPS TLPNNVPAQE
1210 1220 1230 1240 1250
EHGEEPGRDV LEPTQQELAA AAVPVLAKTE VGQEGEVDWL DGEKVKEEQE
1260 1270 1280 1290 1300
VFVHSGPNSQ KAADVTYDSE VMGVAGCQEK ESTEVQSLSL EEGEMETDVE
1310 1320 1330 1340 1350
KEKRETKPEQ VSEEGEQETA APEHEGTYGK PVLTLDMPSS ERGKALGSLG
1360 1370 1380 1390 1400
GSPSLPDQDK AGCIEVQVQS LDTTVTQTAE AVEKVIETVV ISETGESPEC
1410 1420 1430 1440 1450
VGAHLLPAEK SSATGGHWTL QHAEDTVPLG PESQAESIPI IVTPAPESTL
1460 1470 1480 1490 1500
HPDLQGEISA SQRERSEEED KPDAGPDADG KESTAIEKVL KAEPEILELE
1510 1520 1530 1540 1550
SKSNKIVLNV IQTAVDQFAR TETAPETHAY DSQTQVPAMQ ADSQGAQQML
1560 1570 1580 1590 1600
DKNESCQDET PSAAAQRGLA SPDRSGGMGS ASEMLAALAV ESAGVKVSIE
1610 1620 1630 1640 1650
KLPPQPKDQK EHAADGPQLQ SLAQAEASAS GNLTKESPDT NGPKLTEEGD
1660 1670 1680
APKVEVQEEE MSTKSVKENK AQAEEDLQEP KGDLAES
Length:1,687
Mass (Da):181,110
Last modified:January 4, 2005 - v1
Checksum:iAC02148E9BD2DB0C
GO
Isoform 2 (identifier: Q5QD51-2) [UniParc]FASTAAdd to basket
Also known as: Beta

The sequence of this isoform differs from the canonical sequence as follows:
     1-80: Missing.
     81-88: EEEVVDED → MLGTITIT

Show »
Length:1,607
Mass (Da):173,064
Checksum:i9FDB949E3BBCB154
GO
Isoform 3 (identifier: Q5QD51-3) [UniParc]FASTAAdd to basket
Also known as: Gamma

The sequence of this isoform differs from the canonical sequence as follows:
     1-105: Missing.

Show »
Length:1,582
Mass (Da):170,191
Checksum:iB36091A71E22B069
GO

Sequence cautioni

The sequence AAD03788 differs from that shown. Reason: Frameshift at position 1544. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti60 – 601T → S in AAD03788 (PubMed:8626441).Curated
Sequence conflicti136 – 1361S → N in AAD03788 (PubMed:8626441).Curated
Sequence conflicti277 – 2771S → N in AAD03788 (PubMed:8626441).Curated
Sequence conflicti1006 – 10061G → S in AAD03788 (PubMed:8626441).Curated
Sequence conflicti1219 – 12191A → T in AAD03788 (PubMed:8626441).Curated
Sequence conflicti1651 – 16511A → R in AAD03788 (PubMed:8626441).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 105105Missing in isoform 3. 1 PublicationVSP_028136Add
BLAST
Alternative sequencei1 – 8080Missing in isoform 2. 1 PublicationVSP_028137Add
BLAST
Alternative sequencei81 – 888EEEVVDED → MLGTITIT in isoform 2. 1 PublicationVSP_028138

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U41453 mRNA. Translation: AAD03788.1. Frameshift.
AY695056 mRNA. Translation: AAV84358.1.
AY695057 mRNA. Translation: AAV84359.1.
AY695058 mRNA. Translation: AAV84360.1.
RefSeqiNP_001028825.1. NM_001033653.1. [Q5QD51-2]
NP_476444.2. NM_057103.2. [Q5QD51-1]
XP_006227910.1. XM_006227848.2. [Q5QD51-3]
UniGeneiRn.122094.

Genome annotation databases

EnsembliENSRNOT00000026482; ENSRNOP00000026483; ENSRNOG00000019549. [Q5QD51-3]
ENSRNOT00000060767; ENSRNOP00000057494; ENSRNOG00000019549. [Q5QD51-1]
ENSRNOT00000083702; ENSRNOP00000070062; ENSRNOG00000019549. [Q5QD51-2]
GeneIDi83425.
KEGGirno:83425.
UCSCiRGD:70988. rat. [Q5QD51-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U41453 mRNA. Translation: AAD03788.1. Frameshift.
AY695056 mRNA. Translation: AAV84358.1.
AY695057 mRNA. Translation: AAV84359.1.
AY695058 mRNA. Translation: AAV84360.1.
RefSeqiNP_001028825.1. NM_001033653.1. [Q5QD51-2]
NP_476444.2. NM_057103.2. [Q5QD51-1]
XP_006227910.1. XM_006227848.2. [Q5QD51-3]
UniGeneiRn.122094.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000057494.

PTM databases

iPTMnetiQ5QD51.
PhosphoSiteiQ5QD51.

Proteomic databases

PaxDbiQ5QD51.
PRIDEiQ5QD51.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000026482; ENSRNOP00000026483; ENSRNOG00000019549. [Q5QD51-3]
ENSRNOT00000060767; ENSRNOP00000057494; ENSRNOG00000019549. [Q5QD51-1]
ENSRNOT00000083702; ENSRNOP00000070062; ENSRNOG00000019549. [Q5QD51-2]
GeneIDi83425.
KEGGirno:83425.
UCSCiRGD:70988. rat. [Q5QD51-1]

Organism-specific databases

CTDi9590.
RGDi70988. Akap12.

Phylogenomic databases

eggNOGiENOG410IFC9. Eukaryota.
ENOG4110Y3C. LUCA.
GeneTreeiENSGT00730000111244.
HOGENOMiHOG000293190.
HOVERGENiHBG050472.
InParanoidiQ5QD51.
KOiK16528.
OMAiFARTETA.
OrthoDBiEOG091G01X9.
PhylomeDBiQ5QD51.
TreeFamiTF105411.

Miscellaneous databases

PROiQ5QD51.

Gene expression databases

BgeeiENSRNOG00000019549.
ExpressionAtlasiQ5QD51. baseline and differential.
GenevisibleiQ5QD51. RN.

Family and domain databases

InterProiIPR028540. AKAP12.
IPR001573. Pkinase-A_anch_WSK-motif.
IPR018459. RII_binding_1.
[Graphical view]
PANTHERiPTHR23209. PTHR23209. 1 hit.
PfamiPF10522. RII_binding_1. 1 hit.
PF03832. WSK. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAKA12_RAT
AccessioniPrimary (citable) accession number: Q5QD51
Secondary accession number(s): Q5QD49, Q5QD50, Q9Z1F7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: January 4, 2005
Last modified: September 7, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.