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Protein

A-kinase anchor protein 12

Gene

Akap12

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC).By similarity

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
A-kinase anchor protein 12
Short name:
AKAP-12
Gene namesi
Name:Akap12
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi70988. Akap12.

Subcellular locationi

  • Cytoplasmcytoskeleton By similarity
  • Membrane By similarity; Lipid-anchor By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00003049422 – 1687A-kinase anchor protein 12Add BLAST1686

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineBy similarity1
Modified residuei11PhosphoserineCombined sources1
Modified residuei18PhosphoserineBy similarity1
Modified residuei22PhosphoserineBy similarity1
Modified residuei27PhosphoserineBy similarity1
Modified residuei136PhosphoserineBy similarity1
Modified residuei204PhosphoserineBy similarity1
Modified residuei235PhosphoserineBy similarity1
Modified residuei245PhosphoserineCombined sources1
Modified residuei268PhosphoserineCombined sources1
Modified residuei271PhosphoserineCombined sources1
Modified residuei274PhosphoserineCombined sources1
Modified residuei304PhosphoserineCombined sources1
Modified residuei331PhosphothreonineCombined sources1
Modified residuei335PhosphoserineCombined sources1
Modified residuei350PhosphoserineCombined sources1
Modified residuei353PhosphotyrosineBy similarity1
Modified residuei371PhosphoserineBy similarity1
Modified residuei469PhosphoserineCombined sources1
Modified residuei491PhosphoserineCombined sources1
Modified residuei507PhosphoserineCombined sources1
Modified residuei509PhosphoserineCombined sources1
Modified residuei541PhosphoserineCombined sources1
Modified residuei544PhosphoserineCombined sources1
Modified residuei585PhosphoserineCombined sources1
Modified residuei599PhosphoserineCombined sources1
Modified residuei614PhosphoserineCombined sources1
Modified residuei616PhosphoserineBy similarity1
Modified residuei629PhosphothreonineCombined sources1
Modified residuei631PhosphoserineCombined sources1
Modified residuei632PhosphoserineBy similarity1
Modified residuei635PhosphoserineCombined sources1
Modified residuei638PhosphoserineCombined sources1
Modified residuei683PhosphoserineCombined sources1
Modified residuei684PhosphoserineBy similarity1
Modified residuei685PhosphoserineBy similarity1
Modified residuei736PhosphoserineCombined sources1
Modified residuei748PhosphoserineBy similarity1
Modified residuei770PhosphoserineCombined sources1
Modified residuei771PhosphoserineCombined sources1
Modified residuei789PhosphoserineBy similarity1
Modified residuei871PhosphothreonineBy similarity1
Modified residuei873PhosphoserineBy similarity1
Cross-linki1030Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Modified residuei1059PhosphoserineCombined sources1
Modified residuei1289PhosphoserineBy similarity1
Modified residuei1348PhosphoserineCombined sources1
Modified residuei1352PhosphoserineCombined sources1
Modified residuei1354PhosphoserineCombined sources1
Modified residuei1543PhosphoserineBy similarity1
Modified residuei1571PhosphoserineCombined sources1
Modified residuei1637PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated by PKC (in vitro).1 Publication

Keywords - PTMi

Isopeptide bond, Lipoprotein, Myristate, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ5QD51.
PRIDEiQ5QD51.

PTM databases

iPTMnetiQ5QD51.
PhosphoSitePlusiQ5QD51.

Expressioni

Gene expression databases

BgeeiENSRNOG00000019549.
ExpressionAtlasiQ5QD51. baseline and differential.
GenevisibleiQ5QD51. RN.

Interactioni

Subunit structurei

Binds to dimeric RII-alpha regulatory subunit of PKC.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000057494.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini591 – 621AKAP 1Add BLAST31
Domaini740 – 770AKAP 2Add BLAST31
Domaini781 – 811AKAP 3Add BLAST31

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni254 – 544Involved in PKC-bindingBy similarityAdd BLAST291
Regioni1498 – 1511RII-bindingBy similarityAdd BLAST14

Sequence similaritiesi

Contains 3 AKAP domains.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IFC9. Eukaryota.
ENOG4110Y3C. LUCA.
GeneTreeiENSGT00730000111244.
HOGENOMiHOG000293190.
HOVERGENiHBG050472.
InParanoidiQ5QD51.
KOiK16528.
OMAiFARTETA.
OrthoDBiEOG091G01X9.
PhylomeDBiQ5QD51.
TreeFamiTF105411.

Family and domain databases

InterProiIPR028540. AKAP12.
IPR001573. Pkinase-A_anch_WSK-motif.
IPR018459. RII_binding_1.
[Graphical view]
PANTHERiPTHR23209. PTHR23209. 1 hit.
PfamiPF10522. RII_binding_1. 1 hit.
PF03832. WSK. 3 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5QD51-1) [UniParc]FASTAAdd to basket
Also known as: Alpha

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGAGSSTEQR SPEQPAGSDT PSELVLSGHG PAAEASGAAG DPADADPATK
60 70 80 90 100
LPQKNGQLST VNGVAEQGDV HVQEENQEGQ EEEVVDEDVG QRESEDVREK
110 120 130 140 150
DRVEEMAANS TAVEDITKDG QEETSEIIEQ IPASESNVEE MVQPAESQAN
160 170 180 190 200
DVGFKKVFKF VGFKFTVKKD KNEKSDTVQL LTVKKDEGEG AEASVGAGDH
210 220 230 240 250
QEPSVETAVG ESASKESELK QSTEKQEGTL KQEQSSTEIP LQAESDQAAE
260 270 280 290 300
EEAKDEGEEK QEKEPTKSPE SPSSPVSSET TSSFKKFFTH GWAGWRKKTS
310 320 330 340 350
FKKSKEDDLE TAEKRKEQEA EKVDEEEKEK TEPASEEQEP AEDTDQARLS
360 370 380 390 400
ADYEKVELPL EDQVGDLEAS SEEKCAPLAT EVFDEKMEAH QEVVAEVHVS
410 420 430 440 450
TVEKTEEEQG GGGEAEGGVV VEGTGESLPP EKLAEPQEVP QEAEPAEELM
460 470 480 490 500
KSREMCVSGG DHTQLTDLSP EEKTLPKHPE GIVSEVEMLS SQERIKVQGS
510 520 530 540 550
PLKKLFSSSG LKKLSGKKQK GKRGGGGDEE PGEYQHIHTE SPESADEQKG
560 570 580 590 600
ESSASSPEEP EETTCLEKGP LEAPQDGEAE EGTTSDGEKK REGITPWASF
610 620 630 640 650
KKMVTPKKRV RRPSESDKEE ELEKVKSATL SSTDSTVSEM QDEVKTVGEE
660 670 680 690 700
QKPEEPKRRV DTSVSWEALI CVGSSKKRAR KASSSDDEGG PRTLGGDSHR
710 720 730 740 750
AEEASKDKEA GTDAVPASTQ EQDQAQGSSS PEPAGSPSEG EGVSTWESFK
760 770 780 790 800
RLVTPRKKSK SKLEEKAEDS SVEQLSTEIE PSREESWVSI KKFIPGRRKK
810 820 830 840 850
RADGKQEQAT VEDSGPVEIN EDDPDVPAVV PLSEYDAVER EKMEAQGNAE
860 870 880 890 900
LPQLLGAVYV SEELSKTLVH TVSVAVIDGT RAVTSVEERS PSWISASVTE
910 920 930 940 950
PLEHTAGEAM PPVEEVTEKD IIAEETPVLT QTLPEGKDAH DDMVTSEVDF
960 970 980 990 1000
TSEAVTATET SEALRTEEVT EASGAEETTD MVSAVSQLTD SPDTTEEATP
1010 1020 1030 1040 1050
VQEVEGGVLD TEEEERQTQA ILQAVADKVK EESQVPATQT VQRTGSKALE
1060 1070 1080 1090 1100
KVEEVEEDSE VLASEKEKDV MPKGPVQEAG AEHLAQGSET GQATPESLEV
1110 1120 1130 1140 1150
PEVTADVDHV ATCQVIKLQQ LMEQAVAPES SETLTDSETN GSTPLADSDT
1160 1170 1180 1190 1200
ADGTQQDETI DSQDSKATAA VRQSQVTEEE AATAQKEEPS TLPNNVPAQE
1210 1220 1230 1240 1250
EHGEEPGRDV LEPTQQELAA AAVPVLAKTE VGQEGEVDWL DGEKVKEEQE
1260 1270 1280 1290 1300
VFVHSGPNSQ KAADVTYDSE VMGVAGCQEK ESTEVQSLSL EEGEMETDVE
1310 1320 1330 1340 1350
KEKRETKPEQ VSEEGEQETA APEHEGTYGK PVLTLDMPSS ERGKALGSLG
1360 1370 1380 1390 1400
GSPSLPDQDK AGCIEVQVQS LDTTVTQTAE AVEKVIETVV ISETGESPEC
1410 1420 1430 1440 1450
VGAHLLPAEK SSATGGHWTL QHAEDTVPLG PESQAESIPI IVTPAPESTL
1460 1470 1480 1490 1500
HPDLQGEISA SQRERSEEED KPDAGPDADG KESTAIEKVL KAEPEILELE
1510 1520 1530 1540 1550
SKSNKIVLNV IQTAVDQFAR TETAPETHAY DSQTQVPAMQ ADSQGAQQML
1560 1570 1580 1590 1600
DKNESCQDET PSAAAQRGLA SPDRSGGMGS ASEMLAALAV ESAGVKVSIE
1610 1620 1630 1640 1650
KLPPQPKDQK EHAADGPQLQ SLAQAEASAS GNLTKESPDT NGPKLTEEGD
1660 1670 1680
APKVEVQEEE MSTKSVKENK AQAEEDLQEP KGDLAES
Length:1,687
Mass (Da):181,110
Last modified:January 4, 2005 - v1
Checksum:iAC02148E9BD2DB0C
GO
Isoform 2 (identifier: Q5QD51-2) [UniParc]FASTAAdd to basket
Also known as: Beta

The sequence of this isoform differs from the canonical sequence as follows:
     1-80: Missing.
     81-88: EEEVVDED → MLGTITIT

Show »
Length:1,607
Mass (Da):173,064
Checksum:i9FDB949E3BBCB154
GO
Isoform 3 (identifier: Q5QD51-3) [UniParc]FASTAAdd to basket
Also known as: Gamma

The sequence of this isoform differs from the canonical sequence as follows:
     1-105: Missing.

Show »
Length:1,582
Mass (Da):170,191
Checksum:iB36091A71E22B069
GO

Sequence cautioni

The sequence AAD03788 differs from that shown. Reason: Frameshift at position 1544.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti60T → S in AAD03788 (PubMed:8626441).Curated1
Sequence conflicti136S → N in AAD03788 (PubMed:8626441).Curated1
Sequence conflicti277S → N in AAD03788 (PubMed:8626441).Curated1
Sequence conflicti1006G → S in AAD03788 (PubMed:8626441).Curated1
Sequence conflicti1219A → T in AAD03788 (PubMed:8626441).Curated1
Sequence conflicti1651A → R in AAD03788 (PubMed:8626441).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0281361 – 105Missing in isoform 3. 1 PublicationAdd BLAST105
Alternative sequenceiVSP_0281371 – 80Missing in isoform 2. 1 PublicationAdd BLAST80
Alternative sequenceiVSP_02813881 – 88EEEVVDED → MLGTITIT in isoform 2. 1 Publication8

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U41453 mRNA. Translation: AAD03788.1. Frameshift.
AY695056 mRNA. Translation: AAV84358.1.
AY695057 mRNA. Translation: AAV84359.1.
AY695058 mRNA. Translation: AAV84360.1.
RefSeqiNP_001028825.1. NM_001033653.1. [Q5QD51-2]
NP_476444.2. NM_057103.2. [Q5QD51-1]
XP_006227910.1. XM_006227848.3. [Q5QD51-3]
UniGeneiRn.122094.

Genome annotation databases

EnsembliENSRNOT00000026482; ENSRNOP00000026483; ENSRNOG00000019549. [Q5QD51-3]
ENSRNOT00000060767; ENSRNOP00000057494; ENSRNOG00000019549. [Q5QD51-1]
ENSRNOT00000083702; ENSRNOP00000070062; ENSRNOG00000019549. [Q5QD51-2]
GeneIDi83425.
KEGGirno:83425.
UCSCiRGD:70988. rat. [Q5QD51-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U41453 mRNA. Translation: AAD03788.1. Frameshift.
AY695056 mRNA. Translation: AAV84358.1.
AY695057 mRNA. Translation: AAV84359.1.
AY695058 mRNA. Translation: AAV84360.1.
RefSeqiNP_001028825.1. NM_001033653.1. [Q5QD51-2]
NP_476444.2. NM_057103.2. [Q5QD51-1]
XP_006227910.1. XM_006227848.3. [Q5QD51-3]
UniGeneiRn.122094.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000057494.

PTM databases

iPTMnetiQ5QD51.
PhosphoSitePlusiQ5QD51.

Proteomic databases

PaxDbiQ5QD51.
PRIDEiQ5QD51.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000026482; ENSRNOP00000026483; ENSRNOG00000019549. [Q5QD51-3]
ENSRNOT00000060767; ENSRNOP00000057494; ENSRNOG00000019549. [Q5QD51-1]
ENSRNOT00000083702; ENSRNOP00000070062; ENSRNOG00000019549. [Q5QD51-2]
GeneIDi83425.
KEGGirno:83425.
UCSCiRGD:70988. rat. [Q5QD51-1]

Organism-specific databases

CTDi9590.
RGDi70988. Akap12.

Phylogenomic databases

eggNOGiENOG410IFC9. Eukaryota.
ENOG4110Y3C. LUCA.
GeneTreeiENSGT00730000111244.
HOGENOMiHOG000293190.
HOVERGENiHBG050472.
InParanoidiQ5QD51.
KOiK16528.
OMAiFARTETA.
OrthoDBiEOG091G01X9.
PhylomeDBiQ5QD51.
TreeFamiTF105411.

Miscellaneous databases

PROiQ5QD51.

Gene expression databases

BgeeiENSRNOG00000019549.
ExpressionAtlasiQ5QD51. baseline and differential.
GenevisibleiQ5QD51. RN.

Family and domain databases

InterProiIPR028540. AKAP12.
IPR001573. Pkinase-A_anch_WSK-motif.
IPR018459. RII_binding_1.
[Graphical view]
PANTHERiPTHR23209. PTHR23209. 1 hit.
PfamiPF10522. RII_binding_1. 1 hit.
PF03832. WSK. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAKA12_RAT
AccessioniPrimary (citable) accession number: Q5QD51
Secondary accession number(s): Q5QD49, Q5QD50, Q9Z1F7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: January 4, 2005
Last modified: November 2, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.