Q5Q157 (Q5Q157_9INFA) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names	Submitted name: NP EMBL AAV68025.1
Organism	Influenza A virus (A/swine/Korea/S452/2004(H9N2)) EMBL AAV68025.1
Taxonomic identifier	300749 [NCBI]
Taxonomic lineage	Viruses › ssRNA viruses › ssRNA negative-strand viruses › Orthomyxoviridae › Influenzavirus A › Influenza A virus › H9N2 subtype

Protein attributes

Sequence length	499 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function

Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals and is responsible of the active RNP import into the nucleus through the cellular importin alpha/beta pathway. Later in the infection, nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that the nucleoprotein binds directly exportin-1 (XPO1) and plays an active role in RNP nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmask nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus By similarity. SAAS SAAS002141

Subunit structure

Homomultimerizes to form the nucleocapsid. May bind human exportin-1. Binds to viral genomic RNA. Protein-RNA contacts are mediated by a combination of electrostatic interactions between positively charged residues and the phosphate backbone and planar interactions between aromatic side chains and bases By similarity. SAAS SAAS002141

Ontologies

Keywords
Biological process	Host-virus interaction SAAS SAAS002141 Viral penetration into host nucleus SAAS SAAS002141 Virus entry into host cell
Cellular component	Host nucleus SAAS SAAS002141 Virion SAAS SAAS002141
Ligand	RNA-binding SAAS SAAS002141
Gene Ontology (GO)
Biological_process	viral entry into host cell Inferred from electronic annotation. Source: UniProtKB-KW viral penetration into host nucleus Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	host cell nucleus Inferred from electronic annotation. Source: UniProtKB-KW virion Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q5Q157 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: 1228BFBA9131BCE7	FASTA	499	56,235
	10 20 30 40 50 60 MASQGTKRSY EQMETGGERQ NATEIRASVG RMVGGIGRFY IQMCTELKLS DHEGRLIQNS 70 80 90 100 110 120 ITIERMVLSA FDERRNRYLE EHPSAGKDPK KTGGPIYRRR DGKWMRELIL YDKEEIRRIW 130 140 150 160 170 180 RQANNGEDAT AGLTHIMIWH SNLNDATYQR TRALVRTGMD PRMCSLMQGS TLPRRSGAAG 190 200 210 220 230 240 AAVMGVGTMV MELIRMIKRG IDDRIFWRGE NGPKNKGLHM RECATFSKGS SKQQHKEQWW 250 260 270 280 290 300 TRYEKAGIPG NAEIEDLIFL ARSALILRGS VAHKSCLPAC VYGLAVASGY DFEREGYSLV 310 320 330 340 350 360 GIDPFRLLQN SQVFSLIRPN ENPAHKSQLV WMACHSAAFE DLRVSSFIRG ARVVPRGQLS 370 380 390 400 410 420 TRGVQIASNE NTETMDSSTL ELRSRYWAIR TRSGGNTNQQ RASAGQISVQ PTFSVQRNLP 430 440 450 460 470 480 FERATIMAAF TGSTEGRTSD MRTEIIKMME SARPEDVSFQ GRGVFELSDE KATNPIVPSF 490 DMSKEGSYFF GDNAEEYDN

References

[1]	"Reassorted Swine H9N2 Influenza virus in Korea, 2004." Seo S.-H., Kim J.-A., Jo S.-K. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: A/Swine/Korea/S452/2004 EMBL AAV68025.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AY790308 Genomic RNA. Translation: AAV68025.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4HUU	X-ray	2.00	C/F	367-375	[»]
4HUV	X-ray	2.50	C/F	367-375	[»]
4HUW	X-ray	3.16	I/J/K/L	367-375	[»]
4HUX	X-ray	2.20	C	367-375	[»]
4HV8	X-ray	2.00	E/F	367-375	[»]

ModBase

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Protocols and materials databases

StructuralBiologyKnowledgebase

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Family and domain databases

InterPro

IPR002141. Flu_NP.
[Graphical view]

Pfam

PF00506. Flu_NP. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

Q5Q157_9INFA

Accession

Primary (citable) accession number: Q5Q157

Entry history

Integrated into UniProtKB/TrEMBL:	January 4, 2005
Last sequence update:	January 4, 2005
Last modified:	May 1, 2013
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)