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Q5Q157

- Q5Q157_9INFA

UniProt

Q5Q157 - Q5Q157_9INFA

Protein
Submitted name:

NP

Gene
N/A
Organism
Influenza A virus (A/swine/Korea/S452/2004(H9N2))
Status
Unreviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 45 (01 Oct 2014)
      Sequence version 1 (04 Jan 2005)
      Previous versions | rss
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    Functioni

    Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals and is responsible of the active RNP import into the nucleus through the cellular importin alpha/beta pathway. Later in the infection, nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that the nucleoprotein binds directly exportin-1 (XPO1) and plays an active role in RNP nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmask nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus.SAAS annotation

    GO - Molecular functioni

    1. RNA binding Source: UniProtKB-KW
    2. structural molecule activity Source: InterPro

    GO - Biological processi

    1. viral entry into host cell Source: UniProtKB-KW
    2. viral penetration into host nucleus Source: UniProtKB-KW

    Keywords - Biological processi

    Host-virus interactionSAAS annotation, Viral penetration into host nucleusSAAS annotation, Virus entry into host cell

    Keywords - Ligandi

    RNA-bindingSAAS annotation

    Names & Taxonomyi

    Protein namesi
    Submitted name:
    NPImported
    OrganismiInfluenza A virus (A/swine/Korea/S452/2004(H9N2))Imported
    Taxonomic identifieri300749 [NCBI]
    Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A

    Subcellular locationi

    Virion SAAS annotation. Host nucleus SAAS annotation

    GO - Cellular componenti

    1. helical viral capsid Source: UniProtKB-KW
    2. host cell nucleus Source: UniProtKB-SubCell
    3. intracellular Source: GOC

    Keywords - Cellular componenti

    Capsid protein, Helical capsid proteinSAAS annotation, Host nucleusSAAS annotation, Virion

    Interactioni

    Subunit structurei

    Homomultimerizes to form the nucleocapsid. May bind human exportin-1. Binds to viral genomic RNA. Protein-RNA contacts are mediated by a combination of electrostatic interactions between positively charged residues and the phosphate backbone and planar interactions between aromatic side chains and bases.SAAS annotation
    Homomultimerizes to form the nucleocapsid. May bind human exportin-1. Binds to viral genomic RNA. Protein-RNA contacts are mediated by a combination of electrostatic interactions between positively charged residues and the phosphate backbone and planar interactions between aromatic side chains and bases By similarity.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4HUUX-ray2.00C/F367-375[»]
    4HUVX-ray2.50C/F367-375[»]
    4HUWX-ray3.16I/J/K/L367-375[»]
    4HUXX-ray2.20C367-375[»]
    4HV8X-ray2.00E/F367-375[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Family and domain databases

    InterProiIPR002141. Flu_NP.
    [Graphical view]
    PfamiPF00506. Flu_NP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5Q157-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASQGTKRSY EQMETGGERQ NATEIRASVG RMVGGIGRFY IQMCTELKLS    50
    DHEGRLIQNS ITIERMVLSA FDERRNRYLE EHPSAGKDPK KTGGPIYRRR 100
    DGKWMRELIL YDKEEIRRIW RQANNGEDAT AGLTHIMIWH SNLNDATYQR 150
    TRALVRTGMD PRMCSLMQGS TLPRRSGAAG AAVMGVGTMV MELIRMIKRG 200
    IDDRIFWRGE NGPKNKGLHM RECATFSKGS SKQQHKEQWW TRYEKAGIPG 250
    NAEIEDLIFL ARSALILRGS VAHKSCLPAC VYGLAVASGY DFEREGYSLV 300
    GIDPFRLLQN SQVFSLIRPN ENPAHKSQLV WMACHSAAFE DLRVSSFIRG 350
    ARVVPRGQLS TRGVQIASNE NTETMDSSTL ELRSRYWAIR TRSGGNTNQQ 400
    RASAGQISVQ PTFSVQRNLP FERATIMAAF TGSTEGRTSD MRTEIIKMME 450
    SARPEDVSFQ GRGVFELSDE KATNPIVPSF DMSKEGSYFF GDNAEEYDN 499
    Length:499
    Mass (Da):56,235
    Last modified:January 4, 2005 - v1
    Checksum:i1228BFBA9131BCE7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY790308 Genomic RNA. Translation: AAV68025.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY790308 Genomic RNA. Translation: AAV68025.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4HUU X-ray 2.00 C/F 367-375 [» ]
    4HUV X-ray 2.50 C/F 367-375 [» ]
    4HUW X-ray 3.16 I/J/K/L 367-375 [» ]
    4HUX X-ray 2.20 C 367-375 [» ]
    4HV8 X-ray 2.00 E/F 367-375 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR002141. Flu_NP.
    [Graphical view ]
    Pfami PF00506. Flu_NP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Reassorted Swine H9N2 Influenza virus in Korea, 2004."
      Seo S.-H., Kim J.-A., Jo S.-K.
      Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: A/Swine/Korea/S452/2004Imported.
    2. "Preemptive priming readily overcomes structure-based mechanisms of virus escape."
      Valkenburg S.A., Gras S., Guillonneau C., Hatton L.A., Bird N.A., Twist K.A., Halim H., Jackson D.C., Purcell A.W., Turner S.J., Doherty P.C., Rossjohn J., Kedzierska K.
      Proc. Natl. Acad. Sci. U.S.A. 110:5570-5575(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 367-375.

    Entry informationi

    Entry nameiQ5Q157_9INFA
    AccessioniPrimary (citable) accession number: Q5Q157
    Entry historyi
    Integrated into UniProtKB/TrEMBL: January 4, 2005
    Last sequence update: January 4, 2005
    Last modified: October 1, 2014
    This is version 45 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported

    External Data

    Dasty 3