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Reviewed, UniProtKB/Swiss-Prot Q5PU49 (KPCD_CANFA)

Last modified October 13, 2009. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Protein kinase C delta type
    EC=2.7.11.13
Alternative name(s):
    nPKC-delta
Gene names
Name: PRKCD
OrganismCanis familiaris (Dog)
Taxonomic identifier9615 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis

Protein attributes

Sequence length674 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

General annotation (Comments)

Function

This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. May play a role in antigen-dependent control of B-cell function. Phosphorylates MUC1 in the C-terminal and regulates the interaction between MUC1 and beta-catenin By similarity.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation

Three specific sites; Thr-505 (activation loop of the kinase domain), Ser-643 (turn motif) and Ser-662 (hydrophobic region), need to be phosphorylated for its full activation.

Subunit structure

Interacts with MUC1, PDK1, RAD9A and CDCP1 By similarity.

Subcellular location

Cytoplasm By similarity. Membrane; Peripheral membrane protein By similarity.

Domain

The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor.

The C2 domain is a non-calcium binding domain. It binds proteins containing phosphotyrosine in a sequence-specific manner By similarity.

Post-translational modification

Phosphorylated on Thr-505, within the activation loop. Autophosphorylated and/or phosphorylated. Although the Thr-505 phosphorylation occurs it is not a prerequisite for enzymatic activity By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.

Contains 1 AGC-kinase C-terminal domain.

Contains 1 C2 domain.

Contains 2 phorbol-ester/DAG-type zinc fingers.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 674674Protein kinase C delta type
PRO_0000055693

Regions

Domain1 – 9090C2
Domain347 – 601255Protein kinase
Domain602 – 67372AGC-kinase C-terminal
Zinc finger158 – 20851Phorbol-ester/DAG-type 1
Zinc finger230 – 28051Phorbol-ester/DAG-type 2
Nucleotide binding353 – 3619ATP By similarity

Sites

Active site4711Proton acceptor By similarity
Binding site3761ATP By similarity
Site481Interaction with phosphotyrosine-containing peptide By similarity
Site621Interaction with phosphotyrosine-containing peptide By similarity
Site671Interaction with phosphotyrosine-containing peptide By similarity
Site1231Interaction with phosphotyrosine-containing peptide By similarity

Amino acid modifications

Modified residue431Phosphothreonine By similarity
Modified residue501Phosphothreonine By similarity
Modified residue1301Phosphoserine By similarity
Modified residue2991Phosphoserine By similarity
Modified residue3021Phosphoserine By similarity
Modified residue3101Phosphotyrosine By similarity
Modified residue3321Phosphotyrosine By similarity
Modified residue5051Phosphothreonine; by PDPK1 By similarity
Modified residue6431Phosphoserine Probable
Modified residue6521Phosphoserine By similarity
Modified residue6621Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5PU49-1 [UniParc].

Last modified January 4, 2005. Version 1.
Checksum: 5A25DADDB7A364BA

FASTA67477,338
        10         20         30         40         50         60 
MAPFLRIAFT SYELGSLQAA DEASQPFCAV KMKEALSTER GKTLVQKKPT MYPEWKSTFD 

        70         80         90        100        110        120 
AHIYEGRVIQ IVLMRAAEEP MSEVTVGVSV LAERCKKNNX KAEFWLDLQP QAKVLMSVQY 

       130        140        150        160        170        180 
FLEDIDCRQS MHGEDEAKLP TMNRRGAIKQ AKIHYIKNHE FIATFFGQPT FCSVCKDFVW 

       190        200        210        220        230        240 
GLNKQGYKCR QCNAAIHKKC IDKIIGRCTG TAANSRDTIF QKERFNIDMP HRFKVYNYMS 

       250        260        270        280        290        300 
PTFCDHCGSL LWGLVKQGLK CEDCGMNVHH KCQKKVANLC GINQKLLAEA LNQVTQRSSR 

       310        320        330        340        350        360 
KSETESVGIY QNFERKPGVS GDIAPGEDNG TYGKIWEGST RCNIDNFIFH KVLGKGSFGK 

       370        380        390        400        410        420 
VLLVELKGKK EFFAIKALKK DVVLIDDDVE CTMVEKRVLA LAWENPFLTH LFCTFQTKDH 

       430        440        450        460        470        480 
LFFVMEFLNG GDLMYHIQDK GRFELYRATF YAAEIVCGLQ FLHNKGIIYR DLKLDNVMLD 

       490        500        510        520        530        540 
QDGHIKIADF GMCKENIFGE KQASTFCGTP DYIAPEILQG LKYSFSVDWW SFGVLLYEML 

       550        560        570        580        590        600 
IGQSPFHGDD EDELFESIRV DTPHYPRWIT KESKDILEKL LERDTTKRLG VTGNIKIHPF 

       610        620        630        640        650        660 
FKTINWTLLE KRAVEPPFKP KVKSPGDYSN FDQEFLNEKA RLSYTDKNLI DSMDQTAFAG 

       670 
FSFVNPKFER FLEK 

« Hide

References

[1]"Canine protein kinase C delta."
Hsieh Y.-T., Chen H.-C.
Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases

AY825360 mRNA. Translation: AAV80465.1.
RefSeqNP_001008716.1.
UniGeneCfa.16226

3D structure databases

SMRQ5PU49. Positions 1-123, 231-280, 344-616.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ5PU49.

Genome annotation databases

EnsemblENSCAFT00000013836; ENSCAFP00000012799; ENSCAFG00000008689; Canis familiaris. [Genome view]
GeneID494005.
KEGGcfa:494005.

Organism-specific databases

CTD494005.

Phylogenomic databases

HOVERGENQ5PU49.

Enzyme and pathway databases

BRENDA2.7.11.13. 463.

Family and domain databases

InterProIPR000961. AGC-kinase_C.
IPR000008. C2_Ca-dep.
IPR020454. DAG/PE_bd.
IPR015745. PKC.
IPR017892. Pkinase_C.
IPR014376. Prot_kin_PKC_delta.
IPR002219. Prot_Kinase_C-like_PE/DAG_bd.
IPR000719. Prot_kinase_core.
IPR017441. Protein_kinase_ATP_BS.
IPR017442. Se/Thr_pkinase-rel.
IPR008271. Ser_thr_pkin_AS.
IPR002290. Ser_thr_pkinase.
[Graphical view]
PANTHERPTHR22985:SF86. PKC. 1 hit.
PfamPF00130. C1_1. 2 hits.
PF00069. Pkinase. 1 hit.
PF00433. Pkinase_C. 1 hit.
[Graphical view]
PIRSFPIRSF000551. PKC_delta. 1 hit.
PRINTSPR00008. DAGPEDOMAIN.
ProDomPD000001. Prot_kinase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00109. C1. 2 hits.
SM00239. C2. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
PROSITEPS51285. AGC_KINASE_CTER. 1 hit.
PS50004. C2. False negative.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKPCD_CANFA
AccessionPrimary (citable) accession number: Q5PU49
Entry history
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: January 4, 2005
Last modified: October 13, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents