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Q5PRF9 (SMAG2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein Smaug homolog 2

Short name=Smaug 2
Short name=hSmaug2
Alternative name(s):
Sterile alpha motif domain-containing protein 4B
Short name=SAM domain-containing protein 4B
Gene names
Name:SAMD4B
Synonyms:SMAUG2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length694 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Has transcriptional repressor activity. Overexpression inhibits the transcriptional activities of AP-1, p53/TP53 and CDKN1A. Ref.1

Subcellular location

Cytoplasm. Nucleus Ref.1.

Tissue specificity

Widely expressed in embryonic and adult tissues. Ref.1

Sequence similarities

Belongs to the SMAUG family.

Contains 1 SAM (sterile alpha motif) domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentCytoplasm
Nucleus
   Molecular functionRepressor
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processregulation of transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from direct assay. Source: HPA

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionpoly(A) RNA binding

Inferred from direct assay PubMed 22681889. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 694694Protein Smaug homolog 2
PRO_0000260080

Regions

Domain299 – 37274SAM

Amino acid modifications

Modified residue2711Phosphoserine Ref.6
Modified residue4071Phosphothreonine Ref.5
Modified residue5551Phosphoserine Ref.8
Modified residue5571Phosphoserine Ref.8
Modified residue5631Phosphoserine Ref.8
Modified residue5921Phosphoserine Ref.6 Ref.7 Ref.8
Modified residue6001Phosphoserine Ref.6 Ref.8

Sequences

Sequence LengthMass (Da)Tools
Q5PRF9 [UniParc].

Last modified January 4, 2005. Version 1.
Checksum: 4AA4769F38494E56

FASTA69475,483
        10         20         30         40         50         60 
MMFRDQVGIL AGWFKGWNEC EQTVALLSLL KRVTRTQARF LQLCLEHSLA DCNDIHLLES 

        70         80         90        100        110        120 
EANSAAIVSQ WQQESKEKVV SLLLSHLPLL QPGNTEAKSE YMRLLQKVLA YSIESNAFIE 

       130        140        150        160        170        180 
ESRQLLSYAL IHPATTLEDR NALALWLSHL EERLASGFRS RPEPSYHSRQ GSDEWGGPAE 

       190        200        210        220        230        240 
LGPGEAGPGW QDKPPRENGH VPFHPSSSVP PAINSIGSNA NTGLPCQIHP SPLKRSMSLI 

       250        260        270        280        290        300 
PTSPQVPGEW PSPEELGARA AFTTPDHAPL SPQSSVASSG SEQTEEQGSS RNTFQEDGSG 

       310        320        330        340        350        360 
MKDVPSWLKS LRLHKYAALF SQMSYEEMMT LTEQHLESQN VTKGARHKIA LSIQKLRERQ 

       370        380        390        400        410        420 
SVLKSLEKDV LEGGNLRNAL QELQQIIITP IKAYSVLQAT VAAATTTPTA KDGAPGEPPL 

       430        440        450        460        470        480 
PGAEPPLAHP GTDKGTEAKD PPAVENYPPP PAPAPTDGSE PAPAPVADGD IPSQFTRVMG 

       490        500        510        520        530        540 
KVCTQLLVSR PDEENITSYL QLIEKCLTHE AFTETQKKRL LSWKQQVLKL LRTFPRKAAL 

       550        560        570        580        590        600 
EMQNYRQQKG WAFGSNSLPI AGSVGMGVAR RTQRQFPMPP RALPPGRMGL LSPSGIGGVS 

       610        620        630        640        650        660 
PRHALTSPSL GGQGRQNLWF ANPGGSNSMP SQSRSSVQRT HSLPVHSSPQ AILMFPPDCP 

       670        680        690 
VPGPDLEINP TLESLCLSMT EHALGDGTDK TSTI 

« Hide

References

« Hide 'large scale' references
[1]"SAMD4B, a novel SAM-containing protein, inhibits AP-1-, p53- and p21-mediated transcriptional activity."
Luo N., Li G., Li Y., Fan X., Wang Y., Ye X., Mo X., Zhou J., Yuan W., Tan M., Xie H., Ocorr K., Bodmer R., Deng Y., Wu X.
BMB Rep. 43:355-361(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, FUNCTION, SUBCELLULAR LOCATION.
Tissue: Heart.
[2]Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Ovary and Uterus.
[4]"Mammalian Smaug is a translational repressor that forms cytoplasmic foci similar to stress granules."
Baez M.V., Boccaccio G.L.
J. Biol. Chem. 280:43131-43140(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION.
[5]"A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.
Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-407, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[6]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-271; SER-592 AND SER-600, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[7]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-592, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[8]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-555; SER-557; SER-563; SER-592 AND SER-600, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[9]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
EF601121 mRNA. Translation: ABQ85549.1.
CH471126 Genomic DNA. Translation: EAW56877.1.
BC054518 mRNA. Translation: AAH54518.1.
BC065211 mRNA. Translation: AAH65211.1.
CCDSCCDS33020.1.
RefSeqNP_060498.2. NM_018028.2.
XP_005259085.1. XM_005259028.2.
XP_005259086.1. XM_005259029.1.
XP_006723319.1. XM_006723256.1.
UniGeneHs.612332.
Hs.707137.

3D structure databases

ProteinModelPortalQ5PRF9.
SMRQ5PRF9. Positions 300-392.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid120407. 8 interactions.
IntActQ5PRF9. 2 interactions.
MINTMINT-3307444.
STRING9606.ENSP00000317224.

PTM databases

PhosphoSiteQ5PRF9.

Polymorphism databases

DMDM74736136.

Proteomic databases

MaxQBQ5PRF9.
PaxDbQ5PRF9.
PRIDEQ5PRF9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000314471; ENSP00000317224; ENSG00000179134.
GeneID55095.
KEGGhsa:55095.
UCSCuc002ola.3. human.

Organism-specific databases

CTD55095.
GeneCardsGC19P039833.
H-InvDBHIX0015115.
HGNCHGNC:25492. SAMD4B.
HPAHPA016800.
neXtProtNX_Q5PRF9.
PharmGKBPA143485607.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG300236.
HOGENOMHOG000290216.
InParanoidQ5PRF9.
OMAQTEEQGS.
PhylomeDBQ5PRF9.
TreeFamTF324165.

Gene expression databases

ArrayExpressQ5PRF9.
BgeeQ5PRF9.
CleanExHS_SAMD4B.
GenevestigatorQ5PRF9.

Family and domain databases

Gene3D1.10.150.50. 1 hit.
1.25.40.170. 2 hits.
InterProIPR001660. SAM.
IPR013761. SAM/pointed.
IPR021129. SAM_type1.
IPR015327. Smaug_PHAT.
[Graphical view]
PfamPF00536. SAM_1. 1 hit.
[Graphical view]
SMARTSM00454. SAM. 1 hit.
[Graphical view]
SUPFAMSSF47769. SSF47769. 1 hit.
ProtoNetSearch...

Other

ChiTaRSSAMD4B. human.
GenomeRNAi55095.
NextBio58681.
PROQ5PRF9.

Entry information

Entry nameSMAG2_HUMAN
AccessionPrimary (citable) accession number: Q5PRF9
Secondary accession number(s): A5Z0M6, Q6P194
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: January 4, 2005
Last modified: July 9, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM