Reviewed, UniProtKB/Swiss-Prot Q5PRF9 (SMAG2_HUMAN)

Names and origin

Protein names

Recommended name:
    Protein Smaug homolog 2
      Short name=Smaug 2
      Short name=hSmaug2
Alternative name(s):
    Sterile alpha motif domain-containing protein 4B
      Short name=SAM domain-containing protein 4B

Gene names

Name:	SAMD4B
Synonyms:	SMAUG2

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	694 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Sequence similarities

Belongs to the SMAUG family. Contains 1 SAM (sterile alpha motif) domain.

Ontologies

Keywords
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
YWHAB	P31946	1	EBI-1047489,EBI-359815
YWHAG	P61981	1	EBI-1047489,EBI-359832
YWHAQ	P27348	1	EBI-1047489,EBI-359854
YWHAZ	P63104	1	EBI-1047489,EBI-347088

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 694	694	Protein Smaug homolog 2		PRO_0000260080
Regions
	Domain	299 – 372	74	SAM
Amino acid modifications
	Modified residue	264	1	Phosphothreonine Ref.5
	Modified residue	271	1	Phosphoserine Ref.5
	Modified residue	407	1	Phosphothreonine Ref.3
	Modified residue	592	1	Phosphoserine Ref.5 Ref.7
	Modified residue	600	1	Phosphoserine Ref.5
	Modified residue	606	1	Phosphothreonine Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q5PRF9-1 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: 4AA4769F38494E56	FASTA	694	75,483
	10 20 30 40 50 60 MMFRDQVGIL AGWFKGWNEC EQTVALLSLL KRVTRTQARF LQLCLEHSLA DCNDIHLLES 70 80 90 100 110 120 EANSAAIVSQ WQQESKEKVV SLLLSHLPLL QPGNTEAKSE YMRLLQKVLA YSIESNAFIE 130 140 150 160 170 180 ESRQLLSYAL IHPATTLEDR NALALWLSHL EERLASGFRS RPEPSYHSRQ GSDEWGGPAE 190 200 210 220 230 240 LGPGEAGPGW QDKPPRENGH VPFHPSSSVP PAINSIGSNA NTGLPCQIHP SPLKRSMSLI 250 260 270 280 290 300 PTSPQVPGEW PSPEELGARA AFTTPDHAPL SPQSSVASSG SEQTEEQGSS RNTFQEDGSG 310 320 330 340 350 360 MKDVPSWLKS LRLHKYAALF SQMSYEEMMT LTEQHLESQN VTKGARHKIA LSIQKLRERQ 370 380 390 400 410 420 SVLKSLEKDV LEGGNLRNAL QELQQIIITP IKAYSVLQAT VAAATTTPTA KDGAPGEPPL 430 440 450 460 470 480 PGAEPPLAHP GTDKGTEAKD PPAVENYPPP PAPAPTDGSE PAPAPVADGD IPSQFTRVMG 490 500 510 520 530 540 KVCTQLLVSR PDEENITSYL QLIEKCLTHE AFTETQKKRL LSWKQQVLKL LRTFPRKAAL 550 560 570 580 590 600 EMQNYRQQKG WAFGSNSLPI AGSVGMGVAR RTQRQFPMPP RALPPGRMGL LSPSGIGGVS 610 620 630 640 650 660 PRHALTSPSL GGQGRQNLWF ANPGGSNSMP SQSRSSVQRT HSLPVHSSPQ AILMFPPDCP 670 680 690 VPGPDLEINP TLESLCLSMT EHALGDGTDK TSTI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Ovary and Uterus.
[2]	"Mammalian Smaug is a translational repressor that forms cytoplasmic foci similar to stress granules." Baez M.V., Boccaccio G.L. J. Biol. Chem. 280:43131-43140(2005) [PubMed: 16221671] [Abstract] Cited for: IDENTIFICATION.
[3]	"A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed: 16964243] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-407, MASS SPECTROMETRY. Tissue: Epithelium.
[4]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-606, MASS SPECTROMETRY.
[5]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-264; SER-271; SER-592 AND SER-600, MASS SPECTROMETRY.
[6]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[7]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-592, MASS SPECTROMETRY. Tissue: T-cell.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC054518 mRNA. Translation: AAH54518.1. BC065211 mRNA. Translation: AAH65211.1.
IPI	IPI00791720.
RefSeq	NP_060498.2.
UniGene	Hs.612332
3D structure databases
HSSP	HSSP built from PDB template 1OXJ based on UniProtKB Q23972.
SMR	Q5PRF9. Positions 299-530.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q5PRF9. 4 interactions.
STRING	Q5PRF9.
PTM databases
PhosphoSite	Q5PRF9.
Proteomic databases
PRIDE	Q5PRF9.
Genome annotation databases
Ensembl	ENST00000314471; ENSP00000317224; ENSG00000179134; Homo sapiens. [Genome view] ENST00000429637; ENSP00000387732; ENSG00000179134; Homo sapiens. [Genome view]
GeneID	55095.
KEGG	hsa:55095.
UCSC	uc002olb.1. human.
Organism-specific databases
CTD	55095.
GeneCards	GC19P044525.
HGNC	HGNC:25492. SAMD4B.
HPA	HPA016800.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG06547.
HOVERGEN	Q5PRF9.
InParanoid	Q5PRF9.
OMA	RRTQRQF.
OrthoDB	EOG99GP29.
Gene expression databases
ArrayExpress	Q5PRF9.
Bgee	Q5PRF9.
CleanEx	HS_SAMD4B.
Genevestigator	Q5PRF9.
GermOnline	ENSG00000179134. Homo sapiens.
Family and domain databases
InterPro	IPR001660. SAM. IPR010993. SAM_homology. IPR013761. SAM_type. IPR021129. SAM_type1. [Graphical view]
Gene3D	G3DSA:1.10.150.50. SAM_type. 1 hit.
Pfam	PF00536. SAM_1. 1 hit. [Graphical view]
SMART	SM00454. SAM. 1 hit. [Graphical view]
PROSITE	PS50105. SAM_DOMAIN. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	58681.

Entry information

Entry name

SMAG2_HUMAN

Accession

Primary (citable) accession number: Q5PRF9
Secondary accession number(s): Q6P194

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	January 4, 2005
Last modified:	February 9, 2010
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.