Q5PRF9 (SMAG2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein Smaug homolog 2
Short name=Smaug 2
Short name=hSmaug2

Alternative name(s):
Sterile alpha motif domain-containing protein 4B
Short name=SAM domain-containing protein 4B

Gene names

Name:	SAMD4B
Synonyms:	SMAUG2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	694 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has transcriptional repressor activity. Overexpression inhibits the transcriptional activities of AP-1, p53/TP53 and CDKN1A. Ref.1
Subcellular location	Cytoplasm. Nucleus Ref.1.
Tissue specificity	Widely expressed in embryonic and adult tissues. Ref.1
Sequence similarities	Belongs to the SMAUG family. Contains 1 SAM (sterile alpha motif) domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Molecular function	Repressor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 694	694	Protein Smaug homolog 2		PRO_0000260080
Regions
	Domain	299 – 372	74	SAM
Amino acid modifications
	Modified residue	271	1	Phosphoserine Ref.6
	Modified residue	407	1	Phosphothreonine Ref.5
	Modified residue	555	1	Phosphoserine Ref.8
	Modified residue	557	1	Phosphoserine Ref.8
	Modified residue	563	1	Phosphoserine Ref.8
	Modified residue	592	1	Phosphoserine Ref.6 Ref.7 Ref.8
	Modified residue	600	1	Phosphoserine Ref.6 Ref.8

Sequences

	Sequence		Length	Mass (Da)
	Q5PRF9 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: 4AA4769F38494E56	FASTA	694	75,483
	10 20 30 40 50 60 MMFRDQVGIL AGWFKGWNEC EQTVALLSLL KRVTRTQARF LQLCLEHSLA DCNDIHLLES 70 80 90 100 110 120 EANSAAIVSQ WQQESKEKVV SLLLSHLPLL QPGNTEAKSE YMRLLQKVLA YSIESNAFIE 130 140 150 160 170 180 ESRQLLSYAL IHPATTLEDR NALALWLSHL EERLASGFRS RPEPSYHSRQ GSDEWGGPAE 190 200 210 220 230 240 LGPGEAGPGW QDKPPRENGH VPFHPSSSVP PAINSIGSNA NTGLPCQIHP SPLKRSMSLI 250 260 270 280 290 300 PTSPQVPGEW PSPEELGARA AFTTPDHAPL SPQSSVASSG SEQTEEQGSS RNTFQEDGSG 310 320 330 340 350 360 MKDVPSWLKS LRLHKYAALF SQMSYEEMMT LTEQHLESQN VTKGARHKIA LSIQKLRERQ 370 380 390 400 410 420 SVLKSLEKDV LEGGNLRNAL QELQQIIITP IKAYSVLQAT VAAATTTPTA KDGAPGEPPL 430 440 450 460 470 480 PGAEPPLAHP GTDKGTEAKD PPAVENYPPP PAPAPTDGSE PAPAPVADGD IPSQFTRVMG 490 500 510 520 530 540 KVCTQLLVSR PDEENITSYL QLIEKCLTHE AFTETQKKRL LSWKQQVLKL LRTFPRKAAL 550 560 570 580 590 600 EMQNYRQQKG WAFGSNSLPI AGSVGMGVAR RTQRQFPMPP RALPPGRMGL LSPSGIGGVS 610 620 630 640 650 660 PRHALTSPSL GGQGRQNLWF ANPGGSNSMP SQSRSSVQRT HSLPVHSSPQ AILMFPPDCP 670 680 690 VPGPDLEINP TLESLCLSMT EHALGDGTDK TSTI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"SAMD4B, a novel SAM-containing protein, inhibits AP-1-, p53- and p21-mediated transcriptional activity." Luo N., Li G., Li Y., Fan X., Wang Y., Ye X., Mo X., Zhou J., Yuan W., Tan M., Xie H., Ocorr K., Bodmer R., Deng Y., Wu X. BMB Rep. 43:355-361(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, FUNCTION, SUBCELLULAR LOCATION. Tissue: Heart.
[2]	Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Ovary and Uterus.
[4]	"Mammalian Smaug is a translational repressor that forms cytoplasmic foci similar to stress granules." Baez M.V., Boccaccio G.L. J. Biol. Chem. 280:43131-43140(2005) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.
[5]	"A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-407, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[6]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-271; SER-592 AND SER-600, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-592, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[8]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-555; SER-557; SER-563; SER-592 AND SER-600, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	EF601121 mRNA. Translation: ABQ85549.1. CH471126 Genomic DNA. Translation: EAW56877.1. BC054518 mRNA. Translation: AAH54518.1. BC065211 mRNA. Translation: AAH65211.1.
IPI	IPI00791720.
RefSeq	NP_060498.2. NM_018028.2.
UniGene	Hs.741401.
3D structure databases
HSSP	HSSP built from PDB template 1OXJ based on UniProtKB Q23972.
ProteinModelPortal	Q5PRF9.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-3307444.
STRING	9606.ENSP00000317224.
PTM databases
PhosphoSite	Q5PRF9.
Polymorphism databases
DMDM	74736136.
Proteomic databases
PaxDb	Q5PRF9.
PRIDE	Q5PRF9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000314471; ENSP00000317224; ENSG00000179134.
GeneID	55095.
KEGG	hsa:55095.
UCSC	uc002ola.3. human.
Organism-specific databases
CTD	55095.
GeneCards	GC19P039833.
H-InvDB	HIX0015115.
HGNC	HGNC:25492. SAMD4B.
HPA	HPA016800.
neXtProt	NX_Q5PRF9.
PharmGKB	PA143485607.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG300236.
HOGENOM	HOG000290216.
InParanoid	Q5PRF9.
OMA	QTEEQGS.
OrthoDB	EOG4H72B4.
Gene expression databases
ArrayExpress	Q5PRF9.
Bgee	Q5PRF9.
CleanEx	HS_SAMD4B.
Genevestigator	Q5PRF9.
GermOnline	ENSG00000179134. Homo sapiens.
Family and domain databases
Gene3D	1.10.150.50. 1 hit. 1.25.40.170. 2 hits.
InterPro	IPR001660. SAM. IPR013761. SAM/pointed. IPR021129. SAM_type1. IPR015327. Smaug_PHAT. [Graphical view]
Pfam	PF00536. SAM_1. 1 hit. [Graphical view]
SMART	SM00454. SAM. 1 hit. [Graphical view]
SUPFAM	SSF47769. SAM_homology. 1 hit.
PROSITE	PS50105. SAM_DOMAIN. False negative. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	SAMD4B. human.
GenomeRNAi	55095.
NextBio	58681.

Entry information

Entry name

SMAG2_HUMAN

Accession

Primary (citable) accession number: Q5PRF9
Secondary accession number(s): A5Z0M6, Q6P194

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	January 4, 2005
Last modified:	May 1, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.