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Protein

Cholesterol 25-hydroxylase-like protein

Gene

ch25h

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes. Plays a key role in cell positioning and movement in lymphoid tissues: 25-hydroxycholesterol is an intermediate in biosynthesis of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC), an oxysterol that acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for a number of lymphoid cells. May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. In testis, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation.By similarity

Catalytic activityi

Cholesterol + AH2 + O2 = 25-hydroxycholesterol + A + H2O.By similarity

Cofactori

Fe cationBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Keywords - Ligandi

Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cholesterol 25-hydroxylase-like protein (EC:1.14.99.-)
Gene namesi
Name:ch25h
ORF Names:zgc:101688
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 12

Organism-specific databases

ZFINiZDB-GENE-041212-81. ch25h.

Subcellular locationi

  • Endoplasmic reticulum membrane By similarity; Multi-pass membrane protein By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei22 – 4221HelicalSequence analysisAdd
BLAST
Transmembranei69 – 8921HelicalSequence analysisAdd
BLAST
Transmembranei108 – 12821HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 251251Cholesterol 25-hydroxylase-like proteinPRO_0000226805Add
BLAST

Proteomic databases

PaxDbiQ5PRC0.

Expressioni

Gene expression databases

BgeeiENSDARG00000045190.

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000066438.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi126 – 1305Histidine box-1
Motifi141 – 1455Histidine box-2
Motifi222 – 2287Histidine box-3

Sequence similaritiesi

Belongs to the sterol desaturase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0873. Eukaryota.
COG3000. LUCA.
GeneTreeiENSGT00530000063017.
HOGENOMiHOG000015767.
InParanoidiQ5PRC0.
KOiK10223.
OMAiYFLWHLL.
OrthoDBiEOG091G0CY4.
PhylomeDBiQ5PRC0.
TreeFamiTF314256.

Family and domain databases

InterProiIPR006694. Fatty_acid_hydroxylase.
[Graphical view]
PfamiPF04116. FA_hydroxylase. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5PRC0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFGLQHIWDC ILQYEAQLRS PFFPVLFSIT VYLSFCLPFV LLDALSPKVE
60 70 80 90 100
LIRRYKIQQK ASVSWTMMWS CLALSLYNHV VYIFPLSVLH WYWRPVSYLA
110 120 130 140 150
EAPGVLRVVW DLAACLLLFD FQYFVWHLLH HKVPWLYRTF HKVHHKYTST
160 170 180 190 200
FALATEYSGA WETLSLGFFA AVNPMLLGVH PMTEMLFHML NMWLSVEDHC
210 220 230 240 250
GYDLPWATHR LMPFGLYGGA PHHDVHHQKF KSNYAPYFTH WDKLFGTLHF

E
Length:251
Mass (Da):29,735
Last modified:January 4, 2005 - v1
Checksum:i15315B70C824E279
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC086721 mRNA. Translation: AAH86721.1.
RefSeqiNP_001008652.1. NM_001008652.1.
UniGeneiDr.82220.

Genome annotation databases

EnsembliENSDART00000066439; ENSDARP00000066438; ENSDARG00000045190.
GeneIDi494109.
KEGGidre:494109.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC086721 mRNA. Translation: AAH86721.1.
RefSeqiNP_001008652.1. NM_001008652.1.
UniGeneiDr.82220.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000066438.

Proteomic databases

PaxDbiQ5PRC0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000066439; ENSDARP00000066438; ENSDARG00000045190.
GeneIDi494109.
KEGGidre:494109.

Organism-specific databases

CTDi9023.
ZFINiZDB-GENE-041212-81. ch25h.

Phylogenomic databases

eggNOGiKOG0873. Eukaryota.
COG3000. LUCA.
GeneTreeiENSGT00530000063017.
HOGENOMiHOG000015767.
InParanoidiQ5PRC0.
KOiK10223.
OMAiYFLWHLL.
OrthoDBiEOG091G0CY4.
PhylomeDBiQ5PRC0.
TreeFamiTF314256.

Miscellaneous databases

PROiQ5PRC0.

Gene expression databases

BgeeiENSDARG00000045190.

Family and domain databases

InterProiIPR006694. Fatty_acid_hydroxylase.
[Graphical view]
PfamiPF04116. FA_hydroxylase. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCH25H_DANRE
AccessioniPrimary (citable) accession number: Q5PRC0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: January 4, 2005
Last modified: September 7, 2016
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.