Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Cholesterol 25-hydroxylase-like protein

Gene

ch25h

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes. Plays a key role in cell positioning and movement in lymphoid tissues: 25-hydroxycholesterol is an intermediate in biosynthesis of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC), an oxysterol that acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for a number of lymphoid cells. May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. In testis, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation.By similarity

Catalytic activityi

Cholesterol + AH2 + O2 = 25-hydroxycholesterol + A + H2O.By similarity

Cofactori

Fe cationBy similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
Biological processLipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism
LigandIron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cholesterol 25-hydroxylase-like protein (EC:1.14.99.-)
Gene namesi
Name:ch25h
ORF Names:zgc:101688
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 12

Organism-specific databases

ZFINiZDB-GENE-041212-81 ch25h

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei22 – 42HelicalSequence analysisAdd BLAST21
Transmembranei69 – 89HelicalSequence analysisAdd BLAST21
Transmembranei108 – 128HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002268051 – 251Cholesterol 25-hydroxylase-like proteinAdd BLAST251

Proteomic databases

PaxDbiQ5PRC0

Expressioni

Gene expression databases

BgeeiENSDARG00000045190
ExpressionAtlasiQ5PRC0 baseline

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000066438

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi126 – 130Histidine box-15
Motifi141 – 145Histidine box-25
Motifi222 – 228Histidine box-37

Sequence similaritiesi

Belongs to the sterol desaturase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0873 Eukaryota
COG3000 LUCA
GeneTreeiENSGT00530000063017
HOGENOMiHOG000015767
InParanoidiQ5PRC0
KOiK10223
OMAiQFNCNFA
OrthoDBiEOG091G0CY4
PhylomeDBiQ5PRC0
TreeFamiTF314256

Family and domain databases

InterProiView protein in InterPro
IPR006694 Fatty_acid_hydroxylase
PfamiView protein in Pfam
PF04116 FA_hydroxylase, 1 hit

Sequencei

Sequence statusi: Complete.

Q5PRC0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFGLQHIWDC ILQYEAQLRS PFFPVLFSIT VYLSFCLPFV LLDALSPKVE
60 70 80 90 100
LIRRYKIQQK ASVSWTMMWS CLALSLYNHV VYIFPLSVLH WYWRPVSYLA
110 120 130 140 150
EAPGVLRVVW DLAACLLLFD FQYFVWHLLH HKVPWLYRTF HKVHHKYTST
160 170 180 190 200
FALATEYSGA WETLSLGFFA AVNPMLLGVH PMTEMLFHML NMWLSVEDHC
210 220 230 240 250
GYDLPWATHR LMPFGLYGGA PHHDVHHQKF KSNYAPYFTH WDKLFGTLHF

E
Length:251
Mass (Da):29,735
Last modified:January 4, 2005 - v1
Checksum:i15315B70C824E279
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC086721 mRNA Translation: AAH86721.1
RefSeqiNP_001008652.1, NM_001008652.1
UniGeneiDr.82220

Genome annotation databases

EnsembliENSDART00000066439; ENSDARP00000066438; ENSDARG00000045190
GeneIDi494109
KEGGidre:494109

Similar proteinsi

Entry informationi

Entry nameiCH25H_DANRE
AccessioniPrimary (citable) accession number: Q5PRC0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: January 4, 2005
Last modified: March 28, 2018
This is version 82 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health