Reviewed, UniProtKB/Swiss-Prot Q5PRA2 (DHTK1_DANRE)

Names and origin

Protein names

Recommended name:
    Probable 2-oxoglutarate dehydrogenase E1 component DHKTD1, mitochondrial
    EC=1.2.4.2
Alternative name(s):
    Dehydrogenase E1 and transketolase domain-containing protein 1

Gene names

Name:	dhtkd1
ORF Names:	zgc:101818

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	920 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3) By similarity.
Catalytic activity	2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine = [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2.
Cofactor	Thiamine pyrophosphate By similarity.
Subcellular location	Mitochondrion Potential.
Sequence similarities	Belongs to the alpha-ketoglutarate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Thiamine pyrophosphate
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	oxoglutarate dehydrogenase (succinyl-transferring) activity Inferred from electronic annotation. Source: EC thiamin pyrophosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 920		Probable 2-oxoglutarate dehydrogenase E1 component DHKTD1, mitochondrial		PRO_0000307940

Sequences

	Sequence		Length	Mass (Da)
	Q5PRA2-1 [UniParc]. Last modified October 23, 2007. Version 2. Checksum: 7753F5D8CB29A2A7	FASTA	920	102,964
	10 20 30 40 50 60 MLIHLPRLLH RSFGARRSFL ASLYHTQRGV YGFRPRDGPE RRAELTQISA LNRDHGLARL 70 80 90 100 110 120 VEAYRAHGHK VAKINPLLPQ SPVLESVPEI SLLNGAVRGV LNTTGLRHFG KAEATAEEVV 130 140 150 160 170 180 AYLESTYCGG ISVETSQLQT LEEREWFADR FEELKKEAFS PEERRMLAKL MLESQEFDHF 190 200 210 220 230 240 LATKFATVKR YGGEGAESMM GFFYELFRSS VYSGVTDVVM GMPHRGRLNL LTGLLQFPPE 250 260 270 280 290 300 LMFRKMRGLS EFPEHSPSIG DVLSHLTSTV ELDFGAGHPL HVTMLPNPSH LEAINPVTQG 310 320 330 340 350 360 KTRGRQQVKQ DGDYSTDPHS RPGDKVICLQ VHGDASFSGQ GIVPETFTLS NLPHYRVGGS 370 380 390 400 410 420 IHLIVNNQVG YTTPSERGRS SLYCSDVGKM VGCAIIHVNG DDAEEVLRAT RLAVEYQRRF 430 440 450 460 470 480 RKDVIVDLLC YRQWGHNELD EPFFTNPAMY KIIRSRKSIP DSYADQLISE GLMTDEERSQ 490 500 510 520 530 540 IKTSYYATLN NQLTNMTLYS PPPTNLQGRW GDLVEPQNRV SCWDTGVAQP LLQFVGAKSV 550 560 570 580 590 600 DIPEEIILHS HLRKTHVQAR LQKLEEGTKL DWSTAEALAF GTLLCQGFNI RISGQDVGRG 610 620 630 640 650 660 TFSQRHAMVV CQETNDMFIP LNHISSEQKG HLEVCNSALS EEAVLGFEYG MSIAQPRLLP 670 680 690 700 710 720 IWEAQFGDFF NGAQIIFDTF LSGGEAKWLL QSGLVILLPH GYDGAGPEHS SCRIERFLQL 730 740 750 760 770 780 CDSKEEGVDG DTVNMAVVNP TLPAQYFHLL RRQMIRNFRK PLIVASPKTL LRFSGAVSGL 790 800 810 820 830 840 SDMGPGTSFK PIIGDSSVNP ASVQRVLFCS GKHYYALLKH RETIPEAEKN TALVRVEELC 850 860 870 880 890 900 PFPTEALQQE LNKYTNAKEF IWSQEEPQNM GCWSFVSPRF EKQLACKLRL VSRPALPAPA 910 920 VGIGTLHHQQ HEAILTSSFS

References

[1]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Olfactory epithelium.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC086742 mRNA. Translation: AAH86742.1. Different initiation.
IPI	IPI00832858.
RefSeq	NP_001008619.1.
UniGene	Dr.117252
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSDARG00000016415. Danio rerio. [Contig view]
GeneID	494076.
KEGG	dre:494076.
Organism-specific databases
ZFIN	ZDB-GENE-041212-44. dhtkd1.
Phylogenomic databases
HOGENOM	Q5PRA2.
HOVERGEN	Q5PRA2.
Enzyme and pathway databases
BRENDA	1.2.4.2. 96826.
Gene expression databases
ArrayExpress	Q5PRA2.
Bgee	Q5PRA2.
Family and domain databases
InterPro	IPR011603. 2oxoglutarate_DH_E1. IPR001017. DH_E1. IPR005475. Transketolase_central-reg. [Graphical view]
PANTHER	PTHR23152. 2oxoglutarate_DH_E1. 1 hit.
Pfam	PF00676. E1_dh. 1 hit. PF02779. Transket_pyr. 1 hit. [Graphical view]
PIRSF	PIRSF000157. Oxoglu_dh_E1. 1 hit.
TIGRFAMs	TIGR00239. 2oxo_dh_E1. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DHTK1_DANRE

Accession

Primary (citable) accession number: Q5PRA2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 23, 2007
Last sequence update:	October 23, 2007
Last modified:	June 16, 2009
This is version 30 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Zebrafish annotation project