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Protein

Probable E3 ubiquitin-protein ligase HERC4

Gene

Herc4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Probable E3 ubiquitin-protein ligase involved in either protein trafficking or in the distribution of cellular structures. Required for spermatozoon maturation and fertility, and for the removal of the cytoplasmic droplet of the spermatozoon. E3 ubiquitin-protein ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer it to targeted substrates.By similarity

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei1025Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Differentiation, Spermatogenesis, Ubl conjugation pathway

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable E3 ubiquitin-protein ligase HERC4 (EC:2.3.2.26)
Alternative name(s):
HECT domain and RCC1-like domain-containing protein 4
HECT-type E3 ubiquitin transferase HERC4
Gene namesi
Name:Herc4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 20

Organism-specific databases

RGDi1310971. Herc4.

Subcellular locationi

  • Cytoplasmcytosol By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002782181 – 1057Probable E3 ubiquitin-protein ligase HERC4Add BLAST1057

Proteomic databases

PaxDbiQ5PQN1.
PRIDEiQ5PQN1.

PTM databases

iPTMnetiQ5PQN1.
PhosphoSitePlusiQ5PQN1.

Expressioni

Gene expression databases

BgeeiENSRNOG00000000381.
GenevisibleiQ5PQN1. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000049495.

Structurei

3D structure databases

ProteinModelPortaliQ5PQN1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati1 – 51RCC1 1Add BLAST51
Repeati52 – 101RCC1 2Add BLAST50
Repeati102 – 154RCC1 3Add BLAST53
Repeati156 – 207RCC1 4Add BLAST52
Repeati208 – 259RCC1 5Add BLAST52
Repeati261 – 311RCC1 6Add BLAST51
Repeati313 – 366RCC1 7Add BLAST54
Domaini730 – 1057HECTPROSITE-ProRule annotationAdd BLAST328

Sequence similaritiesi

Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.PROSITE-ProRule annotation
Contains 7 RCC1 repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0941. Eukaryota.
COG5021. LUCA.
COG5184. LUCA.
GeneTreeiENSGT00860000133671.
HOGENOMiHOG000208452.
HOVERGENiHBG050878.
InParanoidiQ5PQN1.
KOiK10615.
OMAiPYNGQCP.
OrthoDBiEOG091G028I.
PhylomeDBiQ5PQN1.
TreeFamiTF315189.

Family and domain databases

CDDicd00078. HECTc. 1 hit.
Gene3Di2.130.10.30. 1 hit.
InterProiIPR000569. HECT_dom.
IPR009091. RCC1/BLIP-II.
IPR000408. Reg_chr_condens.
[Graphical view]
PfamiPF00632. HECT. 1 hit.
PF00415. RCC1. 7 hits.
[Graphical view]
PRINTSiPR00633. RCCNDNSATION.
SMARTiSM00119. HECTc. 1 hit.
[Graphical view]
SUPFAMiSSF50985. SSF50985. 1 hit.
SSF56204. SSF56204. 1 hit.
PROSITEiPS50237. HECT. 1 hit.
PS00626. RCC1_2. 3 hits.
PS50012. RCC1_3. 7 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5PQN1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLCWGNASYG QLGLGGIDEE IVLEPRKSDF FVNKKVRDVG CGLRHTVFVL
60 70 80 90 100
DDGTVYTCGC NDLGQLGHEK SRKKPEQVVA LDAQNIVAVS CGEAHTLALN
110 120 130 140 150
DKGQVYAWGL DSDGQLGLQG SEECIRVPRN IKSLSDIQIV QVACGYYHSL
160 170 180 190 200
ALSKASEVFC WGQNKYGQLG LGIECQKQTS PQLIKSLLGI PFMQVAAGGA
210 220 230 240 250
HSFVLTLSGA IFGWGRNKFG QLGLNDENDR YVPNLLKSLR SQKIVYICCG
260 270 280 290 300
EDHTAALTKE GGVFTFGAGG YGQLGHNSTS HEINPRKVFE LMGSIVTQIA
310 320 330 340 350
CGRQHTSAFV PSSGRIYSFG LGGNGQLGTG STSNRKSPFT VKGNWFSYNG
360 370 380 390 400
QCPQDIGSED YFCVKRIFSG GDQSFSHYSS PQSCGPPDDF RRSDPSKQIW
410 420 430 440 450
TVNEALIQKW LSYPSGRFPV EIANEIDGTF SSSGCLNGSF LAVSNDDHYR
460 470 480 490 500
TGTRFSGVDM NAARLLFHRL IQPDHPQISQ QVAASLEKNL IPKLTSSLPD
510 520 530 540 550
VEALRFYLTL PECPLMSDCN NFTTIAIPFG TALVNLEKAP LKVLENWWSV
560 570 580 590 600
LEPPLFLKIV ELFKEVVVHL LKLYKIGIPP SERRIFNSFL HTALKVLEIL
610 620 630 640 650
HRVNERTGQL IQYDKFYIHE VQELIDIRND YINWVQQQAY GVDVSHGITE
660 670 680 690 700
LADIPVTICT YPFVFDAQAK TTLLQTDAVL QMQMAIDQAH RQNVSSLFLP
710 720 730 740 750
VIESVNPCLI LVVRRENIVG DAMEVLRKTK NIDYKKPLKV IFVGEDAVDA
760 770 780 790 800
GGVRKEFFLL IMRELLDPKY GMFRYYEDSR LIWFSDKTFE DSDLFHLIGV
810 820 830 840 850
ICGLAIYNFT IVDLHFPLAL YKKLLKRKPS LDDLKELMPD VGRSMQQLLD
860 870 880 890 900
YPEDDIEETF CLNFTITVEN FGATEVKELV LNGADTAVNK QNRQEFVDAY
910 920 930 940 950
VDYIFNKSVA SLFDAFHAGF HKVCGGKVLL LFQPNELQAM VIGNTNYDWK
960 970 980 990 1000
ELEKNTEYKG EYWAEHPTIK IFWEVFHELP LEKKKQFLLF LTGSDRIPIL
1010 1020 1030 1040 1050
GMKSLKLVIQ STGGGESYLP VSHTCFNLLD LPKYTEKETL RCKLIQAIDH

NEGFSLI
Length:1,057
Mass (Da):118,540
Last modified:January 4, 2005 - v1
Checksum:i4A3C6F2B2BABF39B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC087104 mRNA. Translation: AAH87104.1.
RefSeqiNP_001012074.1. NM_001012074.1.
XP_006256196.1. XM_006256134.3.
XP_017457145.1. XM_017601656.1.
UniGeneiRn.18143.

Genome annotation databases

EnsembliENSRNOT00000083017; ENSRNOP00000072219; ENSRNOG00000061040.
GeneIDi309758.
KEGGirno:309758.
UCSCiRGD:1310971. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC087104 mRNA. Translation: AAH87104.1.
RefSeqiNP_001012074.1. NM_001012074.1.
XP_006256196.1. XM_006256134.3.
XP_017457145.1. XM_017601656.1.
UniGeneiRn.18143.

3D structure databases

ProteinModelPortaliQ5PQN1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000049495.

PTM databases

iPTMnetiQ5PQN1.
PhosphoSitePlusiQ5PQN1.

Proteomic databases

PaxDbiQ5PQN1.
PRIDEiQ5PQN1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000083017; ENSRNOP00000072219; ENSRNOG00000061040.
GeneIDi309758.
KEGGirno:309758.
UCSCiRGD:1310971. rat.

Organism-specific databases

CTDi26091.
RGDi1310971. Herc4.

Phylogenomic databases

eggNOGiKOG0941. Eukaryota.
COG5021. LUCA.
COG5184. LUCA.
GeneTreeiENSGT00860000133671.
HOGENOMiHOG000208452.
HOVERGENiHBG050878.
InParanoidiQ5PQN1.
KOiK10615.
OMAiPYNGQCP.
OrthoDBiEOG091G028I.
PhylomeDBiQ5PQN1.
TreeFamiTF315189.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

PROiQ5PQN1.

Gene expression databases

BgeeiENSRNOG00000000381.
GenevisibleiQ5PQN1. RN.

Family and domain databases

CDDicd00078. HECTc. 1 hit.
Gene3Di2.130.10.30. 1 hit.
InterProiIPR000569. HECT_dom.
IPR009091. RCC1/BLIP-II.
IPR000408. Reg_chr_condens.
[Graphical view]
PfamiPF00632. HECT. 1 hit.
PF00415. RCC1. 7 hits.
[Graphical view]
PRINTSiPR00633. RCCNDNSATION.
SMARTiSM00119. HECTc. 1 hit.
[Graphical view]
SUPFAMiSSF50985. SSF50985. 1 hit.
SSF56204. SSF56204. 1 hit.
PROSITEiPS50237. HECT. 1 hit.
PS00626. RCC1_2. 3 hits.
PS50012. RCC1_3. 7 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHERC4_RAT
AccessioniPrimary (citable) accession number: Q5PQN1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: January 4, 2005
Last modified: November 30, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.