Q5PQL4 (CLP1_RAT) Reviewed, UniProtKB/Swiss-Prot
Last modified May 1, 2013. Version 58. History...
Names and origin
|Protein names||Recommended name:|
Polyribonucleotide 5'-hydroxyl-kinase Clp1
Polyadenylation factor Clp1
Polynucleotide kinase Clp1
Pre-mRNA cleavage complex II protein Clp1
|Organism||Rattus norvegicus (Rat) [Reference proteome]|
|Taxonomic identifier||10116 [NCBI]|
|Taxonomic lineage||Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus|
|Sequence length||425 AA.|
|Protein existence||Evidence at transcript level|
General annotation (Comments)
Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Appears to have roles in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex. Phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA. Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex (RISC). However, endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing By similarity. HAMAP-Rule MF_03035
ATP + 5'-dephospho-DNA = ADP + 5'-phospho-DNA. HAMAP-Rule MF_03035
ATP + 5'-dephospho-RNA = ADP + 5'-phospho-RNA. HAMAP-Rule MF_03035
Magnesium, manganese or nickel By similarity.
Component of the tRNA splicing endonuclease complex, composed of CLP1, TSEN2, TSEN15, TSEN34 and TSEN54. Component of pre-mRNA cleavage complex II (CF-II). Also associates with numerous components of the pre-mRNA cleavage complex I (CF-I/CFIm), including NUDT21, CPSF2, CPSF3, CPSF6 and CPSF7. Interacts with CSTF2 and SYMPK By similarity.
Nucleus By similarity.
Sequence annotation (Features)
|Feature key||Position(s)||Length||Description||Graphical view||Feature identifier|
|Chain||1 – 425||425||Polyribonucleotide 5'-hydroxyl-kinase Clp1 HAMAP-Rule MF_03035||PRO_0000375168|
|Nucleotide binding||124 – 129||6||ATP By similarity|
|Binding site||22||1||ATP By similarity|
|Binding site||62||1||ATP; via carbonyl oxygen By similarity|
|||Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.|
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Brown Norway.
|||"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."|
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
|CH473949 Genomic DNA. Translation: EDL79312.1.|
BC087130 mRNA. Translation: AAH87130.1.
|RefSeq||NP_001009599.1. NM_001009599.1. |
3D structure databases
|SMR||Q5PQL4. Positions 14-423. |
Protein-protein interaction databases
Protocols and materials databases
Genome annotation databases
|Ensembl||ENSRNOT00000009726; ENSRNOP00000009726; ENSRNOG00000007416. |
|UCSC||RGD:1307679. rat. |
|RGD||1307679. Clp1. |
Gene expression databases
Family and domain databases
|HAMAP||MF_03035. Clp1. |
|InterPro||IPR010655. Pre-mRNA_cleavage_cplxII_Clp1. |
|Pfam||PF06807. Clp1. 1 hit. |
|Accession||Primary (citable) accession number: Q5PQL4|
|Entry status||Reviewed (UniProtKB/Swiss-Prot)|
|Annotation program||Chordata Protein Annotation Program|
Index of protein domains and families