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Reviewed, UniProtKB/Swiss-Prot Q5PQL4 (CLP1_RAT)

Last modified November 3, 2009. Version 33. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Polyribonucleotide 5'-hydroxyl-kinase Clp1
    EC=2.7.1.78
Alternative name(s):
    Polynucleotide kinase Clp1
    Pre-mRNA cleavage complex II protein Clp1
Gene names
Name: Clp1
ORF Names: rCG_26812
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length425 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

General annotation (Comments)

Function

Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Appears to have roles in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex. Phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA. Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex (RISC). However endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing By similarity.

Catalytic activity

ATP + 5'-dephospho-DNA = ADP + 5'-phospho-DNA.

ATP + 5'-dephospho-RNA = ADP + 5'-phospho-RNA.

Cofactor

Magnesium, manganese or nickel By similarity.

Subunit structure

Component of the tRNA splicing endonuclease complex, composed of CLP1, TSEN2, TSEN15, TSEN34 and TSEN54. Component of pre-mRNA cleavage complex II (CF-II). Also associates with numerous components of the pre-mRNA cleavage complex I (CF-I/CFIm), including NUDT21, CPSF2, CPSF3, CPSF6 and CPSF7. Interacts with CSTF2 and SYMPK By similarity.

Subcellular location

Nucleus By similarity.

Sequence similarities

Belongs to the Clp1 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 425425Polyribonucleotide 5'-hydroxyl-kinase Clp1
PRO_0000375168

Regions

Nucleotide binding121 – 1288ATP Potential

Sequences

Sequence LengthMass (Da)Tools
Q5PQL4-1 [UniParc].

Last modified January 4, 2005. Version 1.
Checksum: C1AED7978B383097

FASTA42547,738
        10         20         30         40         50         60 
MSEESNDDKK PTTKFELERE TELRFEVEAS QSVQLELLAG MAEIFGTELT RNKKFTFDAG 

        70         80         90        100        110        120 
AKVAVFTWHG CSLQLSGRTE VAYVSKDTPM LLYLNTHTAL EQMRRQAEKE EERGPRVMVV 

       130        140        150        160        170        180 
GPTDVGKSTV CRLLLNYAVR LGRRPTYVEL DVGQGSVSIP GTMGALYIER PADVEEGFSI 

       190        200        210        220        230        240 
QAPLVYHFGS TTPGTNIKLY NKITSRLADV FNQRCEVNRR ASVSGCVINT CGWVKGYGYQ 

       250        260        270        280        290        300 
ALVHAASAFE VDVVVVLDQE RLYNELKRDL PHFVRTVLLP KSGGVVERSK DFRRECRDER 

       310        320        330        340        350        360 
IREYFYGFRG CFYPHAFNVK FSDVKIYKVG APTIPDSCLP LGMSQEDNQL KLVPVTPGRD 

       370        380        390        400        410        420 
MVHHLLSVST AEGTEENLSE TSVAGFIVVT SVDVEHQVFT VLSPAPRPLP KNFLLIMDIR 


FMDLK 

« Hide

References

[1]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Brown Norway.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.
+Additional computationally mapped references.

Cross-references

Sequence databases

CH473949 Genomic DNA. Translation: EDL79312.1.
BC087130 mRNA. Translation: AAH87130.1.
IPIIPI00193975.
RefSeqNP_001009599.1.
UniGeneRn.144332

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGQ5PQL4.

Genome annotation databases

EnsemblENSRNOT00000009726; ENSRNOP00000009726; ENSRNOG00000007416; Rattus norvegicus. [Genome view]
GeneID311166.
KEGGrno:311166.
UCSCNM_001009599. rat.

Organism-specific databases

CTD311166.
RGD1307679. Clp1.

Phylogenomic databases

HOVERGENQ5PQL4.
OMAVVLLPKS.

Gene expression databases

ArrayExpressQ5PQL4.
GenevestigatorQ5PQL4.

Family and domain databases

InterProIPR010655. Clp1.
[Graphical view]
PfamPF06807. Clp1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio663086.

Entry information

Entry nameCLP1_RAT
AccessionPrimary (citable) accession number: Q5PQL4
Entry history
Integrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: January 4, 2005
Last modified: November 3, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents