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Protein

BRCA1-A complex subunit RAP80

Gene

Uimc1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Ubiquitin-binding protein. Specifically recognizes and binds 'Lys-63'-linked ubiquitin. Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator

Keywords - Biological processi

DNA damage, DNA repair, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-RNO-5693565. Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks.
R-RNO-5693571. Nonhomologous End-Joining (NHEJ).
R-RNO-5693607. Processing of DNA double-strand break ends.
R-RNO-69473. G2/M DNA damage checkpoint.

Names & Taxonomyi

Protein namesi
Recommended name:
BRCA1-A complex subunit RAP80
Alternative name(s):
Receptor-associated protein 80
Ubiquitin interaction motif-containing protein 1
Gene namesi
Name:Uimc1
Synonyms:Rap80
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 17

Organism-specific databases

RGDi1307009. Uimc1.

Subcellular locationi

  • Nucleus By similarity

  • Note: Localizes at sites of DNA damage at double-strand breaks (DSBs).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 726726BRCA1-A complex subunit RAP80PRO_0000373952Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki20 – 20Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei29 – 291PhosphoserineBy similarity
Cross-linki31 – 31Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei44 – 441PhosphoserineCombined sources
Modified residuei46 – 461PhosphoserineCombined sources
Modified residuei51 – 511PhosphothreonineBy similarity
Modified residuei140 – 1401PhosphoserineBy similarity
Modified residuei205 – 2051PhosphoserineBy similarity
Modified residuei379 – 3791PhosphoserineCombined sources
Modified residuei402 – 4021PhosphoserineBy similarity
Modified residuei427 – 4271PhosphoserineBy similarity
Cross-linki551 – 551Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki569 – 569Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki616 – 616Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei636 – 6361PhosphoserineBy similarity
Modified residuei664 – 6641PhosphoserineCombined sources
Modified residuei688 – 6881PhosphoserineCombined sources

Post-translational modificationi

Sumoylated.By similarity
Phosphorylated upon DNA damage by ATM or ATR.By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ5PQK4.
PRIDEiQ5PQK4.

PTM databases

iPTMnetiQ5PQK4.
PhosphoSiteiQ5PQK4.

Expressioni

Gene expression databases

BgeeiENSRNOG00000016891.
ExpressionAtlasiQ5PQK4. baseline and differential.
GenevisibleiQ5PQK4. RN.

Interactioni

Subunit structurei

Interacts with TSP57 (By similarity). Component of the ARISC complex, at least composed of UIMC1/RAP80, FAM175A/Abraxas, BRCC3/BRCC36, BRE/BRCC45 and BABAM1/NBA1. Component of the BRCA1-A complex, at least composed of the BRCA1, BARD1, UIMC1/RAP80, FAM175A/Abraxas, BRCC3/BRCC36, BRE/BRCC45 and BABAM1/NBA1. In the BRCA1-A complex, interacts directly with FAM175A/Abraxas. Interacts with ESR1, NR6A1 and UBE2I (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000022704.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini80 – 9920UIM 1PROSITE-ProRule annotationAdd
BLAST
Domaini104 – 12421UIM 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 101101Necessary for transcriptional repressionBy similarityAdd
BLAST
Regioni97 – 1037UIM-linker
Regioni100 – 200101Necessary for interaction with NR6A1 N-terminusBy similarityAdd
BLAST
Regioni270 – 400131AIRAdd
BLAST
Regioni400 – 507108Necessary for interaction with NR6A1 C-terminusBy similarityAdd
BLAST
Regioni512 – 58978Zinc-finger-like regionAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi60 – 7819LR motifAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi81 – 10828Glu-richAdd
BLAST

Domaini

The tandem UIM domains form a continuous 60 Angstrom-long alpha-helix and mediate binding to 'Lys-63'-linked ubiquitins. UIM1 and UIM2 bind to the proximal and distal ubiquitin moieties and recognize an 'Ile-44'-centered hydrophobic patch. Since UIMs don't interact with the 'Lys-63' isopeptide bond the UIM-linker region between the 2 UIM domains determines the selectivity for 'Lys-63'-linkage, and its length is very important for specificity.By similarity
The Abraxas-interacting region (AIR) mediates the interaction with FAM175A/Abraxas.By similarity

Sequence similaritiesi

Belongs to the RAP80 family.Curated
Contains 2 UIM (ubiquitin-interacting motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IGE5. Eukaryota.
ENOG4111MI8. LUCA.
GeneTreeiENSGT00390000007635.
HOGENOMiHOG000154772.
InParanoidiQ5PQK4.
PhylomeDBiQ5PQK4.
TreeFamiTF336575.

Family and domain databases

InterProiIPR003903. UIM_dom.
[Graphical view]
SMARTiSM00726. UIM. 2 hits.
[Graphical view]
PROSITEiPS50330. UIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5PQK4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRRKKKIKE ASEGQNLEKK DLETTSSVSI KKKRRLEDLF IVISDSDGEE
60 70 80 90 100
TKEENGLQKM KTKQSNRSKC LAKRKIAQMS EEEQFALALK MSEQEAKEVN
110 120 130 140 150
NQEEKEEELL RKAIAESLNS RWSSDASAAR PRPLSTGPSS HPHQDSTKDS
160 170 180 190 200
GTTEGIWQLA PPSLCKGSQI SQGNEAEEGK EPWDHSENPE EEPLSGSSGS
210 220 230 240 250
QDQSSRPVFE NEKVKCFDRC TGHLAEHTQC GKPQESTGSG CAFPKAVQGR
260 270 280 290 300
GDTSRQCLPT PADTKGLQDI GGTVHYYWGV PFCPAGVDPD QYTSVILCQL
310 320 330 340 350
EVYQKSLKMA QRQLVKKRGF GEPVLPRPPF LIQNECGQEE QTSEKHEGLS
360 370 380 390 400
EDVRAGAREE RQGSGASVWH SETKDSQKSP ITSLKQRLLL EEEPTTGRGQ
410 420 430 440 450
SSQGLFVEET SEEVLKSSEG DDAVPASQSI AVLTSKRDLV LMPEASTEEV
460 470 480 490 500
TVCPETQLSS FEPLDLNGED SVDGREIPTE LGMTVSNRQV DNREAGKDSR
510 520 530 540 550
PPAVSASARV SCPLCNQDFP PTKIEQHAMY CTGLMEPETV LTRRRREAKK
560 570 580 590 600
KSDGRTAAQP ALDISRKEKC YLCKSLVPLG EYQCHVETCL QLANVDRENG
610 620 630 640 650
TEGMRRPRVC APVEGKPKQR LRKSKEKGHS QGRLLSLLEQ SEHRTTGVER
660 670 680 690 700
TPKCSEAGAF RMPSPDVEEA GCSRERQSSL SKLNLNESPI KSFVPVSEAT
710 720
NCLVDFKGQF AFRSQTKSGR GRRRKS
Length:726
Mass (Da):80,381
Last modified:January 4, 2005 - v1
Checksum:i2F1DC767FC0B2744
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH474032 Genomic DNA. Translation: EDL94013.1.
BC087149 mRNA. Translation: AAH87149.1.
RefSeqiNP_001013906.1. NM_001013884.1.
UniGeneiRn.103261.

Genome annotation databases

EnsembliENSRNOT00000022703; ENSRNOP00000022704; ENSRNOG00000016891.
GeneIDi290997.
KEGGirno:290997.
UCSCiRGD:1307009. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH474032 Genomic DNA. Translation: EDL94013.1.
BC087149 mRNA. Translation: AAH87149.1.
RefSeqiNP_001013906.1. NM_001013884.1.
UniGeneiRn.103261.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000022704.

PTM databases

iPTMnetiQ5PQK4.
PhosphoSiteiQ5PQK4.

Proteomic databases

PaxDbiQ5PQK4.
PRIDEiQ5PQK4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000022703; ENSRNOP00000022704; ENSRNOG00000016891.
GeneIDi290997.
KEGGirno:290997.
UCSCiRGD:1307009. rat.

Organism-specific databases

CTDi51720.
RGDi1307009. Uimc1.

Phylogenomic databases

eggNOGiENOG410IGE5. Eukaryota.
ENOG4111MI8. LUCA.
GeneTreeiENSGT00390000007635.
HOGENOMiHOG000154772.
InParanoidiQ5PQK4.
PhylomeDBiQ5PQK4.
TreeFamiTF336575.

Enzyme and pathway databases

ReactomeiR-RNO-5693565. Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks.
R-RNO-5693571. Nonhomologous End-Joining (NHEJ).
R-RNO-5693607. Processing of DNA double-strand break ends.
R-RNO-69473. G2/M DNA damage checkpoint.

Miscellaneous databases

PROiQ5PQK4.

Gene expression databases

BgeeiENSRNOG00000016891.
ExpressionAtlasiQ5PQK4. baseline and differential.
GenevisibleiQ5PQK4. RN.

Family and domain databases

InterProiIPR003903. UIM_dom.
[Graphical view]
SMARTiSM00726. UIM. 2 hits.
[Graphical view]
PROSITEiPS50330. UIM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUIMC1_RAT
AccessioniPrimary (citable) accession number: Q5PQK4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: January 4, 2005
Last modified: September 7, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.