Q5PQA4 (NB5R2_XENLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-cytochrome b5 reductase 2
Short name=b5R.2
EC=1.6.2.2

Gene names

Name:

cyb5r2

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	296 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	NADH-cytochrome b5 reductases are involved in desaturation and elongation of fatty acids, cholesterol biosynthesis and drug metabolism By similarity.
Catalytic activity	NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5.
Cofactor	FAD By similarity.
Subcellular location	Membrane; Single-pass membrane protein Potential.
Sequence similarities	Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cytochrome-b5 reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 296	296	NADH-cytochrome b5 reductase 2		PRO_0000287553
Regions
	Transmembrane	2 – 24	23	Helical; Potential
	Domain	35 – 147	113	FAD-binding FR-type
	Nucleotide binding	127 – 142	16	FAD By similarity
	Nucleotide binding	166 – 201	36	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5PQA4 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: BC391EEE22573505	FASTA	296	32,664
	10 20 30 40 50 60 MLVALAAIGV TVLLFLIKAL GSGAKKAPVT LLDPNAKYPL PLIEKQEISH DTKKFRFGLP 70 80 90 100 110 120 SAEHVLGLPV GQHIYLSAKV NGSLVVRAYT PVSSDEVKGH VDLVVKVYYK NVNPKFPDGG 130 140 150 160 170 180 KMSQHLDSLK IGETIDFRGP NGLLVYKGKG KFAIRPDKKA EPKIKVAKHV GMLAGGTGIT 190 200 210 220 230 240 PMLQLIRQIT QDPNDNTKCY LIFANQTEDD ILLRYELETV AKSHPEQFKL WYTLDRPPQG 250 260 270 280 290 WKYGSGFVTA DMIKEHLPPP SEDVLVLMCG PPPMIQFACQ DNLTKLGYPE AGRFAY

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC087294 mRNA. Translation: AAH87294.1.
RefSeq	NP_001088670.1. NM_001095201.1.
UniGene	Xl.49849.
3D structure databases
HSSP	HSSP built from PDB template 1NDH based on UniProtKB P83686.
ProteinModelPortal	Q5PQA4.
SMR	Q5PQA4. Positions 29-296.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	495932.
KEGG	xla:495932.
Organism-specific databases
CTD	51700.
Xenbase	XB-GENE-1006047. cyb5r2.
Phylogenomic databases
HOVERGEN	HBG052580.
Family and domain databases
InterPro	IPR017927. Fd_Rdtase_FAD-bd. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR001834. NADH-Cyt_B5_reductase. IPR008333. OxRdtase_FAD-bd_dom. IPR001433. OxRdtase_FAD/NAD-bd. IPR017938. Riboflavin_synthase-like_b-brl. [Graphical view]
KO	K00326.
Pfam	PF00970. FAD_binding_6. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00406. CYTB5RDTASE. PR00371. FPNCR.
SUPFAM	SSF63380. Riboflavin_synthase_like_b-brl. 1 hit.
PROSITE	PS51384. FAD_FR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NB5R2_XENLA

Accession

Primary (citable) accession number: Q5PQA4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	January 4, 2005
Last modified:	November 16, 2011
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program