Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating

Gene

Nsdhl

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in the sequential removal of two C-4 methyl groups in post-squalene cholesterol biosynthesis.By similarity

Catalytic activityi

A 3-beta-hydroxysteroid-4-alpha-carboxylate + NAD(P)+ = a 3-oxosteroid + CO2 + NAD(P)H.

Pathwayi: zymosterol biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes zymosterol from lanosterol.
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Lanosterol 14-alpha demethylase (Cyp51a1)
  2. no protein annotated in this organism
  3. Methylsterol monooxygenase 1 (Msmo1)
  4. Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating (Nsdhl)
  5. 3-keto-steroid reductase (Hsd17b7)
  6. no protein annotated in this organism
This subpathway is part of the pathway zymosterol biosynthesis, which is itself part of Steroid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes zymosterol from lanosterol, the pathway zymosterol biosynthesis and in Steroid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei161Proton acceptorBy similarity1
Binding sitei165NADBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processCholesterol biosynthesis, Cholesterol metabolism, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism
LigandNAD

Enzyme and pathway databases

ReactomeiR-RNO-191273. Cholesterol biosynthesis.
SABIO-RKiQ5PPL3.
UniPathwayiUPA00770; UER00757.

Names & Taxonomyi

Protein namesi
Recommended name:
Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating (EC:1.1.1.170)
Gene namesi
Name:Nsdhl
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome X

Organism-specific databases

RGDi1359337. Nsdhl.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei289 – 309HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Lipid droplet, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003278311 – 362Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylatingAdd BLAST362

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ5PPL3.
PRIDEiQ5PPL3.

Expressioni

Gene expression databases

BgeeiENSRNOG00000057814.
GenevisibleiQ5PPL3. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000022985.

Structurei

3D structure databases

ProteinModelPortaliQ5PPL3.
SMRiQ5PPL3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi359 – 362Prevents secretion from ERBy similarity4

Sequence similaritiesi

Belongs to the 3-beta-HSD family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1430. Eukaryota.
COG0451. LUCA.
GeneTreeiENSGT00550000074557.
HOGENOMiHOG000167989.
HOVERGENiHBG054675.
InParanoidiQ5PPL3.
KOiK07748.
OMAiHYYSCER.
OrthoDBiEOG091G0BS8.
PhylomeDBiQ5PPL3.
TreeFamiTF354279.

Family and domain databases

InterProiView protein in InterPro
IPR002225. 3Beta_OHSteriod_DH/Estase.
IPR036291. NAD(P)-bd_dom_sf.
PfamiView protein in Pfam
PF01073. 3Beta_HSD. 1 hit.
SUPFAMiSSF51735. SSF51735. 2 hits.

Sequencei

Sequence statusi: Complete.

Q5PPL3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEQAVRSESK KGQVTGTDLI NEVSKAKKCT VIGGSGFLGQ HMVEQLLSRG
60 70 80 90 100
YAVNVFDVRQ GFDNPRVQFF IGDLCNQQDL YPALKGVSTV FHCASPPSNS
110 120 130 140 150
NNKELFYRVN STGTKTVIET CKEAGVQKLI LTSSASVVFE GVDIKNGTED
160 170 180 190 200
LPYAMKPIDY YTETKILQER AVLDANDPKK NFLTAAIRPH GIFGPRDPQL
210 220 230 240 250
VPVLIDAARK GKMKFMIGNG KNLVDFTFVE NVVHGHILAA EHLSRDAGLG
260 270 280 290 300
GKAFHITNDE PIPFWTFLSR ILTGLNYEAP KYHIPYRVAY YLAFLLSLLV
310 320 330 340 350
MVLSPLIQIQ TTFTPFRVAL AGTFHYYSCE KAKKLIGYRP LVTMDDAVER
360
TVQSFHHLRK DK
Length:362
Mass (Da):40,412
Last modified:January 4, 2005 - v1
Checksum:i35B224A8CA20C114
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC087626 mRNA. Translation: AAH87626.1.
RefSeqiNP_001009399.1. NM_001009399.1.
UniGeneiRn.23620.

Genome annotation databases

EnsembliENSRNOT00000091154; ENSRNOP00000069662; ENSRNOG00000057814.
GeneIDi309262.
KEGGirno:309262.

Similar proteinsi

Entry informationi

Entry nameiNSDHL_RAT
AccessioniPrimary (citable) accession number: Q5PPL3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: January 4, 2005
Last modified: November 22, 2017
This is version 99 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families