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Protein

Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating

Gene

Nsdhl

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in the sequential removal of two C-4 methyl groups in post-squalene cholesterol biosynthesis.By similarity

Catalytic activityi

A 3-beta-hydroxysteroid-4-alpha-carboxylate + NAD(P)+ = a 3-oxosteroid + CO2 + NAD(P)H.

Pathwayi: zymosterol biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes zymosterol from lanosterol.
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Lanosterol 14-alpha demethylase (Cyp51a1)
  2. no protein annotated in this organism
  3. Methylsterol monooxygenase 1 (Msmo1)
  4. Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating (Nsdhl)
  5. 3-keto-steroid reductase (Hsd17b7)
  6. no protein annotated in this organism
This subpathway is part of the pathway zymosterol biosynthesis, which is itself part of Steroid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes zymosterol from lanosterol, the pathway zymosterol biosynthesis and in Steroid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei161Proton acceptorBy similarity1
Binding sitei165NADBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Cholesterol biosynthesis, Cholesterol metabolism, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

ReactomeiR-RNO-191273. Cholesterol biosynthesis.
SABIO-RKQ5PPL3.
UniPathwayiUPA00770; UER00757.

Names & Taxonomyi

Protein namesi
Recommended name:
Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating (EC:1.1.1.170)
Gene namesi
Name:Nsdhl
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome X

Organism-specific databases

RGDi1359337. Nsdhl.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei289 – 309HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Lipid droplet, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003278311 – 362Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylatingAdd BLAST362

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ5PPL3.
PRIDEiQ5PPL3.

Expressioni

Gene expression databases

BgeeiENSRNOG00000017099.
GenevisibleiQ5PPL3. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000022985.

Structurei

3D structure databases

ProteinModelPortaliQ5PPL3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi359 – 362Prevents secretion from ERBy similarity4

Sequence similaritiesi

Belongs to the 3-beta-HSD family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1430. Eukaryota.
COG0451. LUCA.
GeneTreeiENSGT00550000074557.
HOGENOMiHOG000167989.
HOVERGENiHBG054675.
InParanoidiQ5PPL3.
KOiK07748.
OMAiEPMRDQV.
OrthoDBiEOG091G0BS8.
PhylomeDBiQ5PPL3.
TreeFamiTF354279.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002225. 3Beta_OHSteriod_DH/Estase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01073. 3Beta_HSD. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 2 hits.

Sequencei

Sequence statusi: Complete.

Q5PPL3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEQAVRSESK KGQVTGTDLI NEVSKAKKCT VIGGSGFLGQ HMVEQLLSRG
60 70 80 90 100
YAVNVFDVRQ GFDNPRVQFF IGDLCNQQDL YPALKGVSTV FHCASPPSNS
110 120 130 140 150
NNKELFYRVN STGTKTVIET CKEAGVQKLI LTSSASVVFE GVDIKNGTED
160 170 180 190 200
LPYAMKPIDY YTETKILQER AVLDANDPKK NFLTAAIRPH GIFGPRDPQL
210 220 230 240 250
VPVLIDAARK GKMKFMIGNG KNLVDFTFVE NVVHGHILAA EHLSRDAGLG
260 270 280 290 300
GKAFHITNDE PIPFWTFLSR ILTGLNYEAP KYHIPYRVAY YLAFLLSLLV
310 320 330 340 350
MVLSPLIQIQ TTFTPFRVAL AGTFHYYSCE KAKKLIGYRP LVTMDDAVER
360
TVQSFHHLRK DK
Length:362
Mass (Da):40,412
Last modified:January 4, 2005 - v1
Checksum:i35B224A8CA20C114
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC087626 mRNA. Translation: AAH87626.1.
RefSeqiNP_001009399.1. NM_001009399.1.
UniGeneiRn.23620.

Genome annotation databases

EnsembliENSRNOT00000091154; ENSRNOP00000069662; ENSRNOG00000057814.
GeneIDi309262.
KEGGirno:309262.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC087626 mRNA. Translation: AAH87626.1.
RefSeqiNP_001009399.1. NM_001009399.1.
UniGeneiRn.23620.

3D structure databases

ProteinModelPortaliQ5PPL3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000022985.

Proteomic databases

PaxDbiQ5PPL3.
PRIDEiQ5PPL3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000091154; ENSRNOP00000069662; ENSRNOG00000057814.
GeneIDi309262.
KEGGirno:309262.

Organism-specific databases

CTDi50814.
RGDi1359337. Nsdhl.

Phylogenomic databases

eggNOGiKOG1430. Eukaryota.
COG0451. LUCA.
GeneTreeiENSGT00550000074557.
HOGENOMiHOG000167989.
HOVERGENiHBG054675.
InParanoidiQ5PPL3.
KOiK07748.
OMAiEPMRDQV.
OrthoDBiEOG091G0BS8.
PhylomeDBiQ5PPL3.
TreeFamiTF354279.

Enzyme and pathway databases

UniPathwayiUPA00770; UER00757.
ReactomeiR-RNO-191273. Cholesterol biosynthesis.
SABIO-RKQ5PPL3.

Miscellaneous databases

PROiQ5PPL3.

Gene expression databases

BgeeiENSRNOG00000017099.
GenevisibleiQ5PPL3. RN.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002225. 3Beta_OHSteriod_DH/Estase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01073. 3Beta_HSD. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiNSDHL_RAT
AccessioniPrimary (citable) accession number: Q5PPL3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: January 4, 2005
Last modified: November 2, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.