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Protein

Deoxyhypusine hydroxylase

Gene

Dohh

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the hydroxylation of the N(6)-(4-aminobutyl)-L-lysine intermediate to form hypusine, an essential post-translational modification only found in mature eIF-5A factor.UniRule annotation

Catalytic activityi

[eIF5A]-deoxyhypusine + AH2 + O2 = [eIF5A]-hypusine + A + H2O.UniRule annotation

Cofactori

Fe2+UniRule annotationNote: Binds 2 Fe2+ ions per subunit.UniRule annotation

Pathwayi: eIF5A hypusination

This protein is involved in the pathway eIF5A hypusination, which is part of Protein modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway eIF5A hypusination and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi56 – 561Iron 1UniRule annotation
Metal bindingi57 – 571Iron 1UniRule annotation
Metal bindingi89 – 891Iron 1UniRule annotation
Metal bindingi90 – 901Iron 1UniRule annotation
Metal bindingi208 – 2081Iron 2UniRule annotation
Metal bindingi209 – 2091Iron 2UniRule annotation
Metal bindingi241 – 2411Iron 2UniRule annotation
Metal bindingi242 – 2421Iron 2UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Hypusine biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

ReactomeiR-RNO-204626. Hypusine synthesis from eIF5A-lysine.
UniPathwayiUPA00354.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyhypusine hydroxylaseUniRule annotation (EC:1.14.99.29UniRule annotation)
Short name:
DOHHUniRule annotation
Alternative name(s):
Deoxyhypusine dioxygenaseUniRule annotation
Deoxyhypusine monooxygenaseUniRule annotation
Gene namesi
Name:Dohh
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi1304783. Dohh.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 302302Deoxyhypusine hydroxylasePRO_0000248577Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ5PPJ4.
PRIDEiQ5PPJ4.

Expressioni

Gene expression databases

BgeeiENSRNOG00000004259.
GenevisibleiQ5PPJ4. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000005640.

Structurei

3D structure databases

ProteinModelPortaliQ5PPJ4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati54 – 8027HEAT-like PBS-type 1Add
BLAST
Repeati87 – 11327HEAT-like PBS-type 2Add
BLAST
Repeati175 – 20127HEAT-like PBS-type 3Add
BLAST
Repeati206 – 23227HEAT-like PBS-type 4Add
BLAST
Repeati239 – 26527HEAT-like PBS-type 5Add
BLAST

Sequence similaritiesi

Belongs to the deoxyhypusine hydroxylase family.UniRule annotation
Contains 5 HEAT-like PBS-type repeats.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0567. Eukaryota.
COG1413. LUCA.
GeneTreeiENSGT00500000044957.
HOGENOMiHOG000248665.
HOVERGENiHBG081460.
InParanoidiQ5PPJ4.
KOiK06072.
OMAiAIDMWEY.
OrthoDBiEOG091G0E2R.
PhylomeDBiQ5PPJ4.
TreeFamiTF105626.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
HAMAPiMF_03101. Deoxyhypusine_hydroxylase. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR027517. Deoxyhypusine_hydroxylase.
IPR004155. PBS_lyase_HEAT.
[Graphical view]
SMARTiSM00567. EZ_HEAT. 6 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5PPJ4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVTEQEVEAI GKTLVDSTQP LQARFRALFT LRGLGGPDAI SWISRGFEDS
60 70 80 90 100
SALLKHELAY CLGQMRDPRA IPVLVSVLQD RNQEPMVRHE AGEALGAIGN
110 120 130 140 150
PKVLGLLKQY STDPVVEVAE TCQLAVRRLE WLQQHPGEAT CAGPYLSVDP
160 170 180 190 200
APPAAEGDVG RLRETLLDEA QPLFERYRAM FALRNVGGKE AALALAEGLK
210 220 230 240 250
CGSALFRHEV GYVLGQLQHE AAVSELAATL ARTTESPMVR HECAEALGAI
260 270 280 290 300
ARPACLAALR EYITDPERVV RESCEVALDM YEYENGQDFQ YADGLERLRP

PP
Length:302
Mass (Da):33,077
Last modified:January 4, 2005 - v1
Checksum:iB29216591C11523D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC087658 mRNA. Translation: AAH87658.1.
RefSeqiNP_001020177.1. NM_001025006.1.
XP_006240999.1. XM_006240937.2.
XP_006241000.1. XM_006240938.2.
XP_006241001.1. XM_006240939.2.
UniGeneiRn.38296.

Genome annotation databases

EnsembliENSRNOT00000005640; ENSRNOP00000005640; ENSRNOG00000004259.
GeneIDi314644.
KEGGirno:314644.
UCSCiRGD:1304783. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC087658 mRNA. Translation: AAH87658.1.
RefSeqiNP_001020177.1. NM_001025006.1.
XP_006240999.1. XM_006240937.2.
XP_006241000.1. XM_006240938.2.
XP_006241001.1. XM_006240939.2.
UniGeneiRn.38296.

3D structure databases

ProteinModelPortaliQ5PPJ4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000005640.

Proteomic databases

PaxDbiQ5PPJ4.
PRIDEiQ5PPJ4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000005640; ENSRNOP00000005640; ENSRNOG00000004259.
GeneIDi314644.
KEGGirno:314644.
UCSCiRGD:1304783. rat.

Organism-specific databases

CTDi83475.
RGDi1304783. Dohh.

Phylogenomic databases

eggNOGiKOG0567. Eukaryota.
COG1413. LUCA.
GeneTreeiENSGT00500000044957.
HOGENOMiHOG000248665.
HOVERGENiHBG081460.
InParanoidiQ5PPJ4.
KOiK06072.
OMAiAIDMWEY.
OrthoDBiEOG091G0E2R.
PhylomeDBiQ5PPJ4.
TreeFamiTF105626.

Enzyme and pathway databases

UniPathwayiUPA00354.
ReactomeiR-RNO-204626. Hypusine synthesis from eIF5A-lysine.

Miscellaneous databases

PROiQ5PPJ4.

Gene expression databases

BgeeiENSRNOG00000004259.
GenevisibleiQ5PPJ4. RN.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
HAMAPiMF_03101. Deoxyhypusine_hydroxylase. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR027517. Deoxyhypusine_hydroxylase.
IPR004155. PBS_lyase_HEAT.
[Graphical view]
SMARTiSM00567. EZ_HEAT. 6 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDOHH_RAT
AccessioniPrimary (citable) accession number: Q5PPJ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: January 4, 2005
Last modified: September 7, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.