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Protein

Enolase-phosphatase E1

Gene

Enoph1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene).UniRule annotation

Catalytic activityi

5-(methylthio)-2,3-dioxopentyl phosphate + H2O = 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: L-methionine biosynthesis via salvage pathway

This protein is involved in step 3 and 4 of the subpathway that synthesizes L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Methylthioribose-1-phosphate isomerase (Mri1)
  2. Methylthioribulose-1-phosphate dehydratase (Apip)
  3. Enolase-phosphatase E1 (Enoph1)
  4. Enolase-phosphatase E1 (Enoph1)
  5. 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (Adi1)
  6. no protein annotated in this organism
This subpathway is part of the pathway L-methionine biosynthesis via salvage pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate, the pathway L-methionine biosynthesis via salvage pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi16MagnesiumUniRule annotation1
Metal bindingi18Magnesium; via carbonyl oxygenUniRule annotation1
Binding sitei184SubstrateUniRule annotation1
Metal bindingi209MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processAmino-acid biosynthesis, Methionine biosynthesis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

ReactomeiR-RNO-1237112 Methionine salvage pathway
UniPathwayiUPA00904; UER00876
UPA00904; UER00877

Names & Taxonomyi

Protein namesi
Recommended name:
Enolase-phosphatase E1UniRule annotation (EC:3.1.3.77UniRule annotation)
Alternative name(s):
2,3-diketo-5-methylthio-1-phosphopentane phosphataseUniRule annotation
MASA homologUniRule annotation
Gene namesi
Name:Enoph1
Synonyms:Masa
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 14

Organism-specific databases

RGDi1309016 Enoph1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002540091 – 261Enolase-phosphatase E1Add BLAST261

Proteomic databases

PaxDbiQ5PPH0
PRIDEiQ5PPH0

PTM databases

iPTMnetiQ5PPH0
PhosphoSitePlusiQ5PPH0

Expressioni

Gene expression databases

BgeeiENSRNOG00000002262
GenevisibleiQ5PPH0 RN

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000003083

Structurei

3D structure databases

ProteinModelPortaliQ5PPH0
SMRiQ5PPH0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni150 – 151Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the HAD-like hydrolase superfamily. MasA/MtnC family.UniRule annotation

Phylogenomic databases

eggNOGiKOG2630 Eukaryota
COG4229 LUCA
GeneTreeiENSGT00440000039914
HOGENOMiHOG000237286
HOVERGENiHBG054539
InParanoidiQ5PPH0
KOiK09880
OMAiHMWRAAY
OrthoDBiEOG091G0IX4
PhylomeDBiQ5PPH0
TreeFamiTF105939

Family and domain databases

CDDicd01629 HAD_EP, 1 hit
Gene3Di3.40.50.1000, 2 hits
HAMAPiMF_01681 Salvage_MtnC, 1 hit
MF_03117 Salvage_MtnC_euk, 1 hit
InterProiView protein in InterPro
IPR023943 Enolase-ppase_E1
IPR027511 ENOPH1_eukaryotes
IPR036412 HAD-like_sf
IPR006439 HAD-SF_hydro_IA
IPR023214 HAD_sf
SUPFAMiSSF56784 SSF56784, 1 hit
TIGRFAMsiTIGR01691 enolase-ppase, 1 hit
TIGR01549 HAD-SF-IA-v1, 1 hit

Sequencei

Sequence statusi: Complete.

Q5PPH0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVVVSVPAEV TVILLDIEGT TTPIAFVKDI LFPYIKENVK EYLQTHWEEE
60 70 80 90 100
ECQQDVSLLR KQAEEDAHLD GAVPIPVASG GDVQQMIQAV VDNVSWQMSH
110 120 130 140 150
DRKTTALKQL QGHMWKAAFT AGRMKAEVFA DVVPAVRRWR EAGMKVYIYS
160 170 180 190 200
SGSVEAQKLL FGHSTEGDIL ELIDGHFDTK IGHKVESDSY RKIADSIGCS
210 220 230 240 250
TNNILFLTDV TVEASAAEEA DVHVAVVVRP GNAGLTDDEK TYYNLISSFS
260
ELYLPSTQSK V
Length:261
Mass (Da):28,875
Last modified:January 4, 2005 - v1
Checksum:i0C51CA857A122BEE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC087697 mRNA Translation: AAH87697.1
RefSeqiNP_001009391.1, NM_001009391.1
UniGeneiRn.98105

Genome annotation databases

EnsembliENSRNOT00000003083; ENSRNOP00000003083; ENSRNOG00000002262
GeneIDi305177
KEGGirno:305177
UCSCiRGD:1309016 rat

Similar proteinsi

Entry informationi

Entry nameiENOPH_RAT
AccessioniPrimary (citable) accession number: Q5PPH0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: January 4, 2005
Last modified: May 23, 2018
This is version 111 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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