Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Enolase-phosphatase E1

Gene

Enoph1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene).UniRule annotation

Catalytic activityi

5-(methylthio)-2,3-dioxopentyl phosphate + H2O = 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: L-methionine biosynthesis via salvage pathway

This protein is involved in step 3 and 4 of the subpathway that synthesizes L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Methylthioribose-1-phosphate isomerase (Mri1)
  2. Methylthioribulose-1-phosphate dehydratase (Apip)
  3. Enolase-phosphatase E1 (Enoph1)
  4. Enolase-phosphatase E1 (Enoph1)
  5. 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (Adi1)
  6. no protein annotated in this organism
This subpathway is part of the pathway L-methionine biosynthesis via salvage pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate, the pathway L-methionine biosynthesis via salvage pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi16 – 161MagnesiumUniRule annotation
Metal bindingi18 – 181Magnesium; via carbonyl oxygenUniRule annotation
Binding sitei184 – 1841SubstrateUniRule annotation
Metal bindingi209 – 2091MagnesiumUniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

ReactomeiR-RNO-1237112. Methionine salvage pathway.
UniPathwayiUPA00904; UER00876.
UPA00904; UER00877.

Names & Taxonomyi

Protein namesi
Recommended name:
Enolase-phosphatase E1UniRule annotation (EC:3.1.3.77UniRule annotation)
Alternative name(s):
2,3-diketo-5-methylthio-1-phosphopentane phosphataseUniRule annotation
MASA homologUniRule annotation
Gene namesi
Name:Enoph1
Synonyms:Masa
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 14

Organism-specific databases

RGDi1309016. Enoph1.

Subcellular locationi

  • Cytoplasm UniRule annotation
  • Nucleus UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 261261Enolase-phosphatase E1PRO_0000254009Add
BLAST

Proteomic databases

PaxDbiQ5PPH0.
PRIDEiQ5PPH0.

PTM databases

iPTMnetiQ5PPH0.
PhosphoSiteiQ5PPH0.

Expressioni

Gene expression databases

BgeeiENSRNOG00000002262.
GenevisibleiQ5PPH0. RN.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000003083.

Structurei

3D structure databases

ProteinModelPortaliQ5PPH0.
SMRiQ5PPH0. Positions 4-254.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni150 – 1512Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the HAD-like hydrolase superfamily. MasA/MtnC family.UniRule annotation

Phylogenomic databases

eggNOGiKOG2630. Eukaryota.
COG4229. LUCA.
GeneTreeiENSGT00440000039914.
HOGENOMiHOG000237286.
HOVERGENiHBG054539.
InParanoidiQ5PPH0.
KOiK09880.
OMAiPYAKQNV.
OrthoDBiEOG091G0IX4.
PhylomeDBiQ5PPH0.
TreeFamiTF105939.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
HAMAPiMF_01681. Salvage_MtnC. 1 hit.
MF_03117. Salvage_MtnC_euk. 1 hit.
InterProiIPR023943. Enolase-ppase_E1.
IPR027511. ENOPH1_eukaryotes.
IPR023214. HAD-like_dom.
IPR006439. HAD-SF_hydro_IA.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01691. enolase-ppase. 1 hit.
TIGR01549. HAD-SF-IA-v1. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5PPH0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVVVSVPAEV TVILLDIEGT TTPIAFVKDI LFPYIKENVK EYLQTHWEEE
60 70 80 90 100
ECQQDVSLLR KQAEEDAHLD GAVPIPVASG GDVQQMIQAV VDNVSWQMSH
110 120 130 140 150
DRKTTALKQL QGHMWKAAFT AGRMKAEVFA DVVPAVRRWR EAGMKVYIYS
160 170 180 190 200
SGSVEAQKLL FGHSTEGDIL ELIDGHFDTK IGHKVESDSY RKIADSIGCS
210 220 230 240 250
TNNILFLTDV TVEASAAEEA DVHVAVVVRP GNAGLTDDEK TYYNLISSFS
260
ELYLPSTQSK V
Length:261
Mass (Da):28,875
Last modified:January 4, 2005 - v1
Checksum:i0C51CA857A122BEE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC087697 mRNA. Translation: AAH87697.1.
RefSeqiNP_001009391.1. NM_001009391.1.
UniGeneiRn.98105.

Genome annotation databases

EnsembliENSRNOT00000003083; ENSRNOP00000003083; ENSRNOG00000002262.
GeneIDi305177.
KEGGirno:305177.
UCSCiRGD:1309016. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC087697 mRNA. Translation: AAH87697.1.
RefSeqiNP_001009391.1. NM_001009391.1.
UniGeneiRn.98105.

3D structure databases

ProteinModelPortaliQ5PPH0.
SMRiQ5PPH0. Positions 4-254.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000003083.

PTM databases

iPTMnetiQ5PPH0.
PhosphoSiteiQ5PPH0.

Proteomic databases

PaxDbiQ5PPH0.
PRIDEiQ5PPH0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000003083; ENSRNOP00000003083; ENSRNOG00000002262.
GeneIDi305177.
KEGGirno:305177.
UCSCiRGD:1309016. rat.

Organism-specific databases

CTDi58478.
RGDi1309016. Enoph1.

Phylogenomic databases

eggNOGiKOG2630. Eukaryota.
COG4229. LUCA.
GeneTreeiENSGT00440000039914.
HOGENOMiHOG000237286.
HOVERGENiHBG054539.
InParanoidiQ5PPH0.
KOiK09880.
OMAiPYAKQNV.
OrthoDBiEOG091G0IX4.
PhylomeDBiQ5PPH0.
TreeFamiTF105939.

Enzyme and pathway databases

UniPathwayiUPA00904; UER00876.
UPA00904; UER00877.
ReactomeiR-RNO-1237112. Methionine salvage pathway.

Miscellaneous databases

PROiQ5PPH0.

Gene expression databases

BgeeiENSRNOG00000002262.
GenevisibleiQ5PPH0. RN.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
HAMAPiMF_01681. Salvage_MtnC. 1 hit.
MF_03117. Salvage_MtnC_euk. 1 hit.
InterProiIPR023943. Enolase-ppase_E1.
IPR027511. ENOPH1_eukaryotes.
IPR023214. HAD-like_dom.
IPR006439. HAD-SF_hydro_IA.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01691. enolase-ppase. 1 hit.
TIGR01549. HAD-SF-IA-v1. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiENOPH_RAT
AccessioniPrimary (citable) accession number: Q5PPH0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: January 4, 2005
Last modified: September 7, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.