Q5PMW8 (OTSA_SALPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha,alpha-trehalose-phosphate synthase [UDP-forming]
EC=2.4.1.15

Alternative name(s):
Osmoregulatory trehalose synthesis protein A
Trehalose-6-phosphate synthase
UDP-glucose-glucosephosphate glucosyltransferase

Gene names

Name:	otsA
Ordered Locus Names:	SPA0940

Organism

Salmonella paratyphi A (strain ATCC 9150 / SARB42) [Complete proteome] [HAMAP]

Taxonomic identifier

295319 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella choleraesuis › Salmonella enterica I › Salmonella paratyphi A

Protein attributes

Sequence length	473 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor By similarity.
Catalytic activity	UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate.
Pathway	Glycan biosynthesis; trehalose biosynthesis.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the glycosyltransferase 20 family.

Ontologies

Keywords
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	trehalose biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 473	473	Alpha,alpha-trehalose-phosphate synthase [UDP-forming]		PRO_0000348917
Regions
	Region	21 – 22	2	UDP-glucose binding By similarity
	Region	365 – 369	5	UDP-glucose binding By similarity
Sites
	Binding site	10	1	Glucose-6-phosphate By similarity
	Binding site	76	1	Glucose-6-phosphate By similarity
	Binding site	130	1	Glucose-6-phosphate By similarity
	Binding site	262	1	UDP-glucose By similarity
	Binding site	267	1	UDP-glucose By similarity
	Binding site	300	1	Glucose-6-phosphate By similarity
	Binding site	339	1	UDP-glucose; via amide nitrogen and carbonyl oxygen By similarity
	Site	85	1	Involved in alpha anomer selectivity By similarity
	Site	155	1	Involved in alpha anomer selectivity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5PMW8 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: E85B3F0AE58E144A	FASTA	473	53,581
	10 20 30 40 50 60 MSRLVVVSNR IAPPDNKGGA GGLAVGVLGA LKAAGGLWFG WSGETGNEDE PLKKVTKGNI 70 80 90 100 110 120 TWASFNLSEQ DYEDYYCQFS NAVLWPAFHY RLDLVQFQRP AWEGYMRVNA LLADKLLPLI 130 140 150 160 170 180 KENDIIWVHD YHLLPFASEL RKRGVNNRIG FFLHIPFPTP EIFNALPPHD ELLEQLCDFD 190 200 210 220 230 240 LLGFQTENDR LAFLDSLSSQ TRVTTRSGKQ HIAWGKDFQT EVYPIGIEPD EIALQAAGPL 250 260 270 280 290 300 PPKLAQLKAE LKNVKNIFSV ERLDYSKGLP ERFLAYEALL ENYPQHRGKI RYTQIAPTSR 310 320 330 340 350 360 GEVQAYQDIR HQLETEAGRI NGKYGQLGWT PLYYLNQHFD RKLLMKIFRY SDVGLVTPLR 370 380 390 400 410 420 DGMNLVAKEF VAAQDPANPG VLVLSQFAGA ANELTSALIV NPYDRDDVAA ALNRALTMPL 430 440 450 460 470 AERISRHAEM LDVIVKNDIN RWQERFIHDL KEVTPRSPER QQQNNVATFP KLA

References

[1]

"Comparison of genome degradation in Paratyphi A and Typhi, human-restricted serovars of Salmonella enterica that cause typhoid." McClelland M., Sanderson K.E., Clifton S.W., Latreille P., Porwollik S., Sabo A., Meyer R., Bieri T., Ozersky P., McLellan M., Harkins C.R., Wang C., Nguyen C., Berghoff A., Elliott G., Kohlberg S., Strong C., Du F. , Carter J., Kremizki C., Layman D., Leonard S., Sun H., Fulton L., Nash W., Miner T., Minx P., Delehaunty K., Fronick C., Magrini V., Nhan M., Warren W., Florea L., Spieth J., Wilson R.K. Nat. Genet. 36:1268-1274(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 9150 / SARB42.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000026 Genomic DNA. Translation: AAV76916.1.
RefSeq	YP_150228.1. NC_006511.1.
3D structure databases
HSSP	HSSP built from PDB template 1UQT based on UniProtKB P31677.
ProteinModelPortal	Q5PMW8.
SMR	Q5PMW8. Positions 2-456.
ModBase	Search...
Protein-protein interaction databases
STRING	295319.SPA0940.
Proteomic databases
PRIDE	Q5PMW8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAV76916; AAV76916; SPA0940.
GeneID	3177676.
KEGG	spt:SPA0940.
PATRIC	32351111. VBISalEnt134188_0992.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0380.
HOGENOM	HOG000191478.
KO	K00697.
OMA	EYGRKEM.
ProtClustDB	PRK10117.
Enzyme and pathway databases
BioCyc	SENT295319:GJBZ-940-MONOMER.
UniPathway	UPA00299.
Family and domain databases
InterPro	IPR001830. Glyco_trans_20. IPR012766. Trehalose_OtsA. [Graphical view]
Pfam	PF00982. Glyco_transf_20. 1 hit. [Graphical view]
TIGRFAMs	TIGR02400. trehalose_OtsA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

OTSA_SALPA

Accession

Primary (citable) accession number: Q5PMW8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	January 4, 2005
Last modified:	May 1, 2013
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program