Reviewed, UniProtKB/Swiss-Prot Q5PIQ0 (DSBD_SALPA)

Names and origin

Protein names

Recommended name:
    Thiol:disulfide interchange protein dsbD
    EC=1.8.1.8
Alternative name(s):
    Protein-disulfide reductase
      Short name=Disulfide reductase

Gene names

Name:	dsbD
Ordered Locus Names:	SPA4139

Organism

Salmonella paratyphi A [Complete proteome] [HAMAP]

Taxonomic identifier

54388 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	567 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Required to facilitate the formation of correct disulfide bonds in some periplasmic proteins and for the assembly of the periplasmic c-type cytochromes. Acts by transferring electrons from cytoplasmic thioredoxin to the periplasm. This transfer involves a cascade of disulfide bond formation and reduction steps By similarity.
Catalytic activity	Protein dithiol + NAD(P)+ = protein disulfide + NAD(P)H. HAMAP MF_00399
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the thioredoxin family. DsbD subfamily. Contains 1 thioredoxin domain.

Ontologies

Keywords
Biological process	Cytochrome c-type biogenesis Electron transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Redox-active center Signal Transmembrane
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro cytochrome complex assembly Inferred from electronic annotation. Source: HAMAP electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: HAMAP protein-disulfide reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 567	548	Thiol:disulfide interchange protein dsbD HAMAP MF_00399		PRO_0000304395
Regions
	Transmembrane	166 – 186	21	Potential
	Transmembrane	211 – 231	21	Potential
	Transmembrane	246 – 266	21	Potential
	Transmembrane	299 – 319	21	Potential
	Transmembrane	326 – 346	21	Potential
	Transmembrane	360 – 380	21	Potential
	Transmembrane	387 – 407	21	Potential
	Transmembrane	418 – 438	21	Potential
	Domain	435 – 567	133	Thioredoxin
Amino acid modifications
	Disulfide bond	122 ↔ 128		Redox-active By similarity
	Disulfide bond	185 ↔ 307		Redox-active By similarity
	Disulfide bond	482 ↔ 485		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5PIQ0-1 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: FF6A6526EB405CBA	FASTA	567	61,314
	10 20 30 40 50 60 MAQRIFTLIL LLCSTSAFAG LFDAPGRSQF VPADRAFVFD FQQNQHDLTL SWQVKEGYYL 70 80 90 100 110 120 YRKQISITPT KADIAAVQLP AGVWHEDEFY GKSEIYRKRL NVPVTVNQAA AGATLTVTYQ 130 140 150 160 170 180 GCADAGFCYP PETKTVPLSE VAAAIDATPT PAVTQTGETS KPAAQLPFSA LWALLIGIGI 190 200 210 220 230 240 AFTPCVLPMY PLISGIVLGG RQRLSTGRAL LLAFIYVQGM ALTYTALGLV VAAAGLQFQA 250 260 270 280 290 300 ALQHPYVLIG LAIVFTLLAL SMFGLFTLQL PSSLQTRLTL MSNRQQGGSP GGVFVMGAIA 310 320 330 340 350 360 GLICSPCTTA PLSAILLYIA QSGNMWLGGG TLYLYALGMG LPLMLVTVFG NRLLPKSGPW 370 380 390 400 410 420 MAHVKTAFGF VILALPVFLL ERIIGEAWGL RLWSLLGVAF FGWAFITSLQ ARRAWMRIVQ 430 440 450 460 470 480 IILLAAALIS VRPLQDWAFG SPSAQAPAHL NFTAISTVDE LNQALAQAKG KPVMLDFYAD 490 500 510 520 530 540 WCVACKEFEK YTFSDPRVQQ ALGDTVLLQA NVTANNAQDV ALLKHLQVLG LPTILFFDAQ 550 560 GQEQPQARVT GFMDAATFSA HLHDRQP

References

[1]

"Comparison of genome degradation in Paratyphi A and Typhi, human-restricted serovars of Salmonella enterica that cause typhoid." McClelland M., Sanderson K.E., Clifton S.W., Latreille P., Porwollik S., Sabo A., Meyer R., Bieri T., Ozersky P., McLellan M., Harkins C.R., Wang C., Nguyen C., Berghoff A., Elliott G., Kohlberg S., Strong C., Du F. , Carter J., Kremizki C., Layman D., Leonard S., Sun H., Fulton L., Nash W., Miner T., Minx P., Delehaunty K., Fronick C., Magrini V., Nhan M., Warren W., Florea L., Spieth J., Wilson R.K. Nat. Genet. 36:1268-1274(2004) [PubMed: 15531882] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 9150 / SARB42.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000026 Genomic DNA. Translation: AAV79880.1.
RefSeq	YP_153192.1.
3D structure databases
SMR	Q5PIQ0. Positions 23-143, 449-567.
ModBase	Search...
Genome annotation databases
GeneID	3176146.
GenomeReviews	Gene locus SPA4139 in contig CP000026_GR.
KEGG	spt:SPA4139.
NMPDR	fig|295319.3.peg.3423.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5PIQ0.
OMA	Q5PIQ0. TITHILW.
Enzyme and pathway databases
BioCyc	SENT295319:SPA4139-MON.
Family and domain databases
HAMAP	MF_00399. [Tree]
InterPro	IPR003834. Cyt_c_assmbl_TM. IPR017936. Thioredoxin-like. IPR015467. Thioredoxin_core. IPR017937. Thioredoxin_CS. IPR013766. Thioredoxin_domain. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
PANTHER	PTHR10438. Trx. 1 hit.
Pfam	PF02683. DsbD. 1 hit. PF00085. Thioredoxin. 1 hit. [Graphical view]
PROSITE	PS00194. THIOREDOXIN_1. 1 hit. PS51352. THIOREDOXIN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DSBD_SALPA

Accession

Primary (citable) accession number: Q5PIQ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	January 4, 2005
Last modified:	June 16, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)