Q5PIG8 (PUR4_SALPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase
Short name=FGAM synthase
Short name=FGAMS
EC=6.3.5.3

Alternative name(s):
Formylglycinamide ribotide amidotransferase
Short name=FGARAT
Formylglycinamide ribotide synthetase

Gene names

Name:	purL
Ordered Locus Names:	SPA0300

Organism

Salmonella paratyphi A [Complete proteome] [HAMAP]

Taxonomic identifier

54388 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	1295 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00419
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00419
Subunit structure	Monomer.
Subcellular location	Cytoplasm HAMAP MF_00419.
Sequence similarities	In the N-terminal section; belongs to the FGAMS family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1295	1295	Phosphoribosylformylglycinamidine synthase HAMAP MF_00419		PRO_0000264592
Regions
	Domain	1042 – 1295	254	Glutamine amidotransferase type-1
	Nucleotide binding	307 – 318	12	ATP Potential
Sites
	Active site	1135	1	Nucleophile By similarity
	Active site	1260	1	By similarity
	Active site	1262	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5PIG8 [UniParc]. Last modified December 4, 2007. Version 3. Checksum: 073DD3960A937C2A	FASTA	1,295	141,443
	10 20 30 40 50 60 MMEILRGSPA LSAFRINKLL ARFQAANLQV HNIYAEYVHF ADLNAPLNDS EQAQLTRLLQ 70 80 90 100 110 120 YGPALNSHTP AGKLLLVTPR PGTISPWSSK ATDIAHNCGL QQVDRLERGV AYYIEASTLT 130 140 150 160 170 180 AEQWRQVAAE LHDRMMETVF SSLTDAEKLF IHHQPAPVSS VDLLGEGRQA LIDANLRLGL 190 200 210 220 230 240 ALAEDEIDYL QEAFTKLGRN PNDIELYMFA QANSEHCRHK IFNADWIIDG KPQPKSLFKM 250 260 270 280 290 300 IKNTFETTPD YVLSAYKDNA AVMEGSAVGR YFADHNTGRY DFHQEPAHIL MKVETHNHPT 310 320 330 340 350 360 AISPWPGAAT GSGGEIRDEG ATGRGAKPKA GLVGFSVSNL RIPGFEQPWE EDFGKPERIV 370 380 390 400 410 420 TALDIMTEGP LGGAAFNNEF GRPALTGYFR TYEDKVNSHN GEELRGYHKP IMLAGGIGNI 430 440 450 460 470 480 RADHVQKGEI VVGAKLIVLG GPAMNIGLGG GAASSMASGQ SDVDLDFASV QRDNPEMERR 490 500 510 520 530 540 CQEVIDRCWQ LGDANPILFI HDVGAGGLSN AMPELVSDGG RGGKFELRDI LSDEPGMSPL 550 560 570 580 590 600 EIWCNESQER YVLAVAADQL PLFDELCKRE RAPYAVIGDA TEEQHLSLHD NHFDNQPIDL 610 620 630 640 650 660 PLDVLLGKTP KMTRDVQTLK AKGDALNRAD ITIADAVNRV LHLPTVAEKT FLVTIGDRTV 670 680 690 700 710 720 TGMVARDQMV GPWQVPVADC AVTTASLDSY YGEAMSIGER APVALLDFAA SARLAVGEAL 730 740 750 760 770 780 TNIAATQIGD IKRIKLSANW MAAAGHPGED AGLYDAVKAV GEELCPQLGL TIPVGKDSMS 790 800 810 820 830 840 MKTRWQEGNE QREMTSPLSL VISAFARVED VRHTLTPQLS TEDNALLLID LGKGHNALGA 850 860 870 880 890 900 TALAQVYRQL GDKPADVRDV AQLKGFYDAM QALVAARKLL AWHDRSDGGL LVTLAEMAFA 910 920 930 940 950 960 GHCGVQVDIA ALGDDHLAAL FNEELGGVIQ VRAEDREAVE ALLAQYGLAD CVHYLGQALA 970 980 990 1000 1010 1020 GDRFVITAND QTVFSESRTT LRVWWAETTW QMQRLRDNPQ CADQEHEAKA NDADPGLNVK 1030 1040 1050 1060 1070 1080 LSFDINEDIA APYIATGARP KVAVLREQGV NSHVEMAAAF HRAGFDAIDV HMSDLLGGRI 1090 1100 1110 1120 1130 1140 GLGNFHALVA CGGFSYGDVL GAGEGWAKSI LFNPRVRDEF ETFFHRPQTL ALGVCNGCQM 1150 1160 1170 1180 1190 1200 MSNLRELIPG SELWPRFVRN HSDRFEARFS LVEVTQSPSL LLQGMVGSQM PIAVSHGEGR 1210 1220 1230 1240 1250 1260 VEVRDDAHLA ALESKGLVAL RYVDNFGKVT ETYPANPNGS PNGITAVTTE NGRVTIMMPH 1270 1280 1290 PERVFRTVAN SWHPENWGED SPWMRIFRNA RKQLG

References

[1]

"Comparison of genome degradation in Paratyphi A and Typhi, human-restricted serovars of Salmonella enterica that cause typhoid." McClelland M., Sanderson K.E., Clifton S.W., Latreille P., Porwollik S., Sabo A., Meyer R., Bieri T., Ozersky P., McLellan M., Harkins C.R., Wang C., Nguyen C., Berghoff A., Elliott G., Kohlberg S., Strong C., Du F. , Carter J., Kremizki C., Layman D., Leonard S., Sun H., Fulton L., Nash W., Miner T., Minx P., Delehaunty K., Fronick C., Magrini V., Nhan M., Warren W., Florea L., Spieth J., Wilson R.K. Nat. Genet. 36:1268-1274(2004) [PubMed: 15531882] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 9150 / SARB42.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000026 Genomic DNA. Translation: AAV76321.1.
RefSeq	YP_149633.1. NC_006511.1.
3D structure databases
HSSP	HSSP built from PDB template 1T3T based on UniProtKB P74881.
ProteinModelPortal	Q5PIG8.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3176257.
GenomeReviews	Gene locus SPA0300 in contig CP000026_GR.
KEGG	spt:SPA0300.
NMPDR	fig|295319.3.peg.1575.
PATRIC	32349715. VBISalEnt134188_0315.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG335309.
OMA	VKAVGME.
ProtClustDB	PRK05297.
Enzyme and pathway databases
BioCyc	SENT295319:SPA0300-MONOMER.
Family and domain databases
HAMAP	MF_00419. PurL_1. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR017926. GATASE_1. IPR010073. PRibForGlyAmidine_synth. IPR022940. PRibForGlyAmidine_synth_bac. IPR016188. PurM_N-like. [Graphical view]
KO	K01952.
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01735. FGAM_synt. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR4_SALPA

Accession

Primary (citable) accession number: Q5PIG8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	December 4, 2007
Last modified:	January 25, 2012
This is version 53 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program