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Protein

Ribosomal RNA small subunit methyltransferase F

Gene

rsmF

Organism
Salmonella paratyphi A (strain ATCC 9150 / SARB42)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + cytosine(1407) in 16S rRNA = S-adenosyl-L-homocysteine + 5-methylcytosine(1407) in 16S rRNA.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei149 – 1491S-adenosyl-L-methionineUniRule annotation
Binding sitei176 – 1761S-adenosyl-L-methionineUniRule annotation
Binding sitei194 – 1941S-adenosyl-L-methionineUniRule annotation
Active sitei247 – 2471NucleophileUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciSENT295319:GJBZ-1023-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase FUniRule annotation (EC:2.1.1.178UniRule annotation)
Alternative name(s):
16S rRNA m5C1407 methyltransferaseUniRule annotation
rRNA (cytosine-C(5)-)-methyltransferase RsmFUniRule annotation
Gene namesi
Name:rsmFUniRule annotation
Ordered Locus Names:SPA1023
OrganismiSalmonella paratyphi A (strain ATCC 9150 / SARB42)
Taxonomic identifieri295319 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000008185 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 479479Ribosomal RNA small subunit methyltransferase FPRO_0000285006Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ5PHN5.
SMRiQ5PHN5. Positions 7-474.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni125 – 1317S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000218115.
KOiK11392.
OMAiWCAEGFW.
OrthoDBiEOG6091D0.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01579. 16SrRNA_methyltr_F.
InterProiIPR018314. Fmu/NOL1/Nop2p_CS.
IPR031341. Methyltr_RsmF_N.
IPR001678. MeTrfase_RsmB/NOP2.
IPR027391. Nol1_Nop2_Fmu_2.
IPR011023. Nop2p.
IPR023267. RCMT.
IPR023545. rRNA_ssu_MeTfrase_F.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01189. Methyltr_RsmB-F. 1 hit.
PF17125. Methyltr_RsmF_N. 1 hit.
PF13636. Methyltranf_PUA. 1 hit.
[Graphical view]
PRINTSiPR02008. RCMTFAMILY.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00446. nop2p. 1 hit.
PROSITEiPS01153. NOL1_NOP2_SUN. 1 hit.
PS51686. SAM_MT_RSMB_NOP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5PHN5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQHAVYFPD AFLTQMREAM PSTLSFDEFI SACQRPLRRS IRINTLKISV
60 70 80 90 100
ADFLALIAPY GWSLTPIPWC HEGFWIERDD EEALPLGSTA EHLSGLFYIQ
110 120 130 140 150
EASSMLPVAA LFADDNHPQR VMDMAAAPGS KTTQIAARMG NRGAILANEF
160 170 180 190 200
SASRVKVLHA NISRCGIANT ALTHFDGRVF GAALPEMFDA ILLDAPCSGE
210 220 230 240 250
GVVRKDPDAL KNWSPESNLD IAATQRELLD SAFHALRPGG TLVYSTCTLN
260 270 280 290 300
RQENEDVCLW LKETYADAVE FLPLGDLFPD ADRALTPEGF LHVFPQIYDC
310 320 330 340 350
EGFFVARLRK MSSLPAMPAP GYKVGAFPFT PLKGREALNV TQAANAVGLL
360 370 380 390 400
WDENLHLWQR EKEVWLFPAE IESLIGKVRF SRLGIKLAES HNKGYRWQHE
410 420 430 440 450
ATIALACPTH AHAFELSAQE AEEWYRGRDI YPQTPPAADD VLVTFQHQPL
460 470
GLAKRIGARI KNSYPRELVR DGKLFTGNS
Length:479
Mass (Da):53,284
Last modified:January 4, 2005 - v1
Checksum:iC44B2E39E5E58990
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000026 Genomic DNA. Translation: AAV76995.1.
RefSeqiWP_011233026.1. NC_006511.1.

Genome annotation databases

EnsemblBacteriaiAAV76995; AAV76995; SPA1023.
KEGGispt:SPA1023.
PATRICi32351315. VBISalEnt134188_1094.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000026 Genomic DNA. Translation: AAV76995.1.
RefSeqiWP_011233026.1. NC_006511.1.

3D structure databases

ProteinModelPortaliQ5PHN5.
SMRiQ5PHN5. Positions 7-474.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAV76995; AAV76995; SPA1023.
KEGGispt:SPA1023.
PATRICi32351315. VBISalEnt134188_1094.

Phylogenomic databases

HOGENOMiHOG000218115.
KOiK11392.
OMAiWCAEGFW.
OrthoDBiEOG6091D0.

Enzyme and pathway databases

BioCyciSENT295319:GJBZ-1023-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01579. 16SrRNA_methyltr_F.
InterProiIPR018314. Fmu/NOL1/Nop2p_CS.
IPR031341. Methyltr_RsmF_N.
IPR001678. MeTrfase_RsmB/NOP2.
IPR027391. Nol1_Nop2_Fmu_2.
IPR011023. Nop2p.
IPR023267. RCMT.
IPR023545. rRNA_ssu_MeTfrase_F.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01189. Methyltr_RsmB-F. 1 hit.
PF17125. Methyltr_RsmF_N. 1 hit.
PF13636. Methyltranf_PUA. 1 hit.
[Graphical view]
PRINTSiPR02008. RCMTFAMILY.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00446. nop2p. 1 hit.
PROSITEiPS01153. NOL1_NOP2_SUN. 1 hit.
PS51686. SAM_MT_RSMB_NOP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 9150 / SARB42.

Entry informationi

Entry nameiRSMF_SALPA
AccessioniPrimary (citable) accession number: Q5PHN5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: January 4, 2005
Last modified: May 11, 2016
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.