Reviewed, UniProtKB/Swiss-Prot Q5PF10 (GSH1_SALPA)

Names and origin

Protein names

Recommended name:
    Glutamate--cysteine ligase
    EC=6.3.2.2
Alternative name(s):
    Gamma-glutamylcysteine synthetase
    Gamma-ECS
      Short name=GCS

Gene names

Name:	gshA
Ordered Locus Names:	SPA2677

Organism

Salmonella paratyphi A [Complete proteome] [HAMAP]

Taxonomic identifier

54388 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	518 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine. HAMAP MF_00578
Pathway	Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2. HAMAP MF_00578
Sequence similarities	Belongs to the glutamate--cysteine ligase type 1 family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Glutathione biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutathione biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-cysteine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 518	518	Glutamate--cysteine ligase HAMAP MF_00578		PRO_0000192537

Sequences

	Sequence		Length	Mass (Da)
	Q5PF10-1 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: 9766882B28965C47	FASTA	518	58,384
	10 20 30 40 50 60 MIPDVSQALA WLEKHPQALK GIQRGLERET LRVNADGTLA TTGHPEALGS ALTHKWITTD 70 80 90 100 110 120 FAEALLEFIT PVDGDIQHML TFMRDLHRYT ARKLGDERMW PLSMPCYIAE GQDIELAQYG 130 140 150 160 170 180 TSNTGRFKTL YREGLKNRYG ALMQTISGVH YNFSLPMAFW QAKCGVTEGE AAKEKISAGY 190 200 210 220 230 240 FRLIRNYYRF GWVIPYLFGA SPAICSSFLQ GKPTTLPFEK TDCGMYYLPY ATSLRLSDLG 250 260 270 280 290 300 YTNKSQSNLG ITFNDLHEYV AGLKRAIKTP SEEYARIGVE KDGKRLQINS NVLQIENELY 310 320 330 340 350 360 APIRPKRVTR SGESPSDALL RGGIEYIEVR SLDINPFSPI GVDEQQVRFL DLFMVWCVLA 370 380 390 400 410 420 DAPEMSSDEL LCTRTNWNRV ILEGRKPGLT LGIGCETAQF PLPKVGKDLF RDLKRVAQTL 430 440 450 460 470 480 DSIHGGEEYQ KVCDELVACF DNPELTFSAR ILRSMIDEGI GGTGKAFGEA YRNLLREEPL 490 500 510 EILQEEEFIA ERDASVRRQQ EIEAADTEPF AAWLAKHA

References

[1]

"Comparison of genome degradation in Paratyphi A and Typhi, human-restricted serovars of Salmonella enterica that cause typhoid." McClelland M., Sanderson K.E., Clifton S.W., Latreille P., Porwollik S., Sabo A., Meyer R., Bieri T., Ozersky P., McLellan M., Harkins C.R., Wang C., Nguyen C., Berghoff A., Elliott G., Kohlberg S., Strong C., Du F. , Carter J., Kremizki C., Layman D., Leonard S., Sun H., Fulton L., Nash W., Miner T., Minx P., Delehaunty K., Fronick C., Magrini V., Nhan M., Warren W., Florea L., Spieth J., Wilson R.K. Nat. Genet. 36:1268-1274(2004) [PubMed: 15531882] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 9150 / SARB42.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000026 Genomic DNA. Translation: AAV78538.1.
RefSeq	YP_151850.1.
3D structure databases
SMR	Q5PF10. Positions 1-514.
ModBase	Search...
Genome annotation databases
GeneID	3177337.
GenomeReviews	Gene locus SPA2677 in contig CP000026_GR.
KEGG	spt:SPA2677.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5PF10.
OMA	Q5PF10. EYIEVRA.
Enzyme and pathway databases
BioCyc	SENT295319:SPA2677-MON.
Family and domain databases
HAMAP	MF_00578. [Tree]
InterPro	IPR007370. Glu_cys_ligase. IPR006334. Glut_cys_ligase. [Graphical view]
Pfam	PF04262. Glu_cys_ligase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01434. glu_cys_ligase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GSH1_SALPA

Accession

Primary (citable) accession number: Q5PF10

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	January 4, 2005
Last modified:	June 16, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)