Q5PEA9 (SPTP_SALPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Secreted effector protein SptP

Including the following 2 domains:

1. GTPase-activating protein
   Short name=GAP
2. Tyrosine-protein phosphatase
   EC=3.1.3.48

Gene names

Name:	sptP
Ordered Locus Names:	SPA2736

Organism

Salmonella paratyphi A (strain ATCC 9150 / SARB42) [Complete proteome] [HAMAP]

Taxonomic identifier

295319 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella choleraesuis › Salmonella enterica I › Salmonella paratyphi A

Protein attributes

Sequence length	543 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Effector proteins function to alter host cell physiology and promote bacterial survival in host tissues. This protein includes tyrosine phosphatase and GTPase activating protein (GAP) activities. After bacterial internalization, GAP mediates the reversal of the cytoskeletal changes induced by SopE. This function is independent of its tyrosine phosphatase activity, which remains unclear By similarity.
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
Subunit structure	Forms a complex with SicP By similarity.
Subcellular location	Secreted By similarity. Host cytoplasm By similarity. Note: Secreted via type III secretion system 1 (SPI-1 TTSS), and delivered into the host cytoplasm By similarity.
Miscellaneous	Requires SicP as a chaperone for its stability and secretion By similarity.
Sequence similarities	In the N-terminal section; belongs to the YopE family. Contains 1 tyrosine-protein phosphatase domain.

Ontologies

Keywords
Biological process	Virulence
Cellular component	Host cytoplasm Secreted
Molecular function	GTPase activation Hydrolase Protein phosphatase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW peptidyl-tyrosine dephosphorylation Inferred from electronic annotation. Source: GOC positive regulation of GTPase activity Inferred from electronic annotation. Source: GOC
Cellular_component	extracellular space Inferred from electronic annotation. Source: InterPro host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTPase activator activity Inferred from electronic annotation. Source: UniProtKB-KW protein tyrosine phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 543	543	Secreted effector protein SptP		PRO_0000094864
Regions
	Domain	280 – 543	264	Tyrosine-protein phosphatase
	Region	35 – 139	105	Chaperone-binding By similarity
	Region	167 – 290	124	GAP By similarity
Sites
	Active site	481	1	Phosphocysteine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5PEA9 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: BE20AFE173E8C1D3	FASTA	543	60,005
	10 20 30 40 50 60 MLRYEERKLN NLTLSSFSKS GVSSDTRLYI AKENTDKAYV APEKFSSKVL TWLGKMPLFK 70 80 90 100 110 120 NTEVVQKHTE NIRVQNQKIL QTFLQALTEK YGEKAVNNAL YMSSINMNKP LTQRLVVQIT 130 140 150 160 170 180 ECVKGADGGF INIIKNKDNV GVMNAALVIK GGDTKVTEQN NDVGAESKQP LLDIALKGLK 190 200 210 220 230 240 RTIPQLEQMD GNSLRENFQE MASGNGPLRS LMTNLQSLNK IPEAKQLNDY VTTLKNIQIG 250 260 270 280 290 300 ADRFSQWGTC GGEVERWIDK ASTHELTQAV KKIHVIAKEL KNVTAEFEKI KAGASMPQTM 310 320 330 340 350 360 NGPTLGLARF AVSSIPINQQ TQVKLSDGMP VPVNTLTFDG KPVALAGSYP KNTPDALEAH 370 380 390 400 410 420 MKMLLEKECS CLAVLTSEDQ MQAKQLPAYF RGSYTFGEVH TNSQKVSSAS QGGAIDQYNM 430 440 450 460 470 480 QLSCGEKRYT IPVLHVKNWP DHQPLPSTDQ LEYLADRVKN SNQNGAPGRS SSDKHLPMIH 490 500 510 520 530 540 CLGGVGRTGT MAAALVLKDN PHSNLEQVRA DFRNSRNNRM LEDASQFVQL KAMQAQLLMT TAS

References

[1]

"Comparison of genome degradation in Paratyphi A and Typhi, human-restricted serovars of Salmonella enterica that cause typhoid." McClelland M., Sanderson K.E., Clifton S.W., Latreille P., Porwollik S., Sabo A., Meyer R., Bieri T., Ozersky P., McLellan M., Harkins C.R., Wang C., Nguyen C., Berghoff A., Elliott G., Kohlberg S., Strong C., Du F. , Carter J., Kremizki C., Layman D., Leonard S., Sun H., Fulton L., Nash W., Miner T., Minx P., Delehaunty K., Fronick C., Magrini V., Nhan M., Warren W., Florea L., Spieth J., Wilson R.K. Nat. Genet. 36:1268-1274(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 9150 / SARB42.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000026 Genomic DNA. Translation: AAV78593.1.
RefSeq	YP_151905.1. NC_006511.1.
3D structure databases
ProteinModelPortal	Q5PEA9.
SMR	Q5PEA9. Positions 36-139, 167-539.
ModBase	Search...
Protein-protein interaction databases
STRING	295319.SPA2736.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAV78593; AAV78593; SPA2736.
GeneID	3177037.
KEGG	spt:SPA2736.
PATRIC	32355024. VBISalEnt134188_2910.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	NOG74835.
HOGENOM	HOG000028733.
KO	K13740.
OMA	WIDKAST.
ProtClustDB	PRK15375.
Enzyme and pathway databases
BioCyc	SENT295319:GJBZ-2734-MONOMER.
Family and domain databases
Gene3D	1.20.120.260. 1 hit.
InterPro	IPR011070. Globular_prot_asu/bsu. IPR015203. SicP-binding. IPR000387. Tyr/Dual-sp_Pase. IPR016130. Tyr_Pase_AS. IPR000242. Tyr_Pase_rcpt/non-rcpt. IPR003546. Tyr_Pase_SptP/YopH. IPR014773. Uncharacterised_dom_YopE. [Graphical view]
Pfam	PF09119. SicP-binding. 1 hit. PF00102. Y_phosphatase. 1 hit. PF03545. YopE. 1 hit. [Graphical view]
PRINTS	PR01371. BACYPHPHTASE.
SMART	SM00194. PTPc. 1 hit. [Graphical view]
SUPFAM	SSF56568. AlphaBeta_subunt. 1 hit. SSF47233. Vir_YopE. 1 hit.
PROSITE	PS00383. TYR_PHOSPHATASE_1. 1 hit. PS50056. TYR_PHOSPHATASE_2. 1 hit. PS50055. TYR_PHOSPHATASE_PTP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPTP_SALPA

Accession

Primary (citable) accession number: Q5PEA9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	January 4, 2005
Last modified:	May 1, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program