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Q5PEA9 (SPTP_SALPA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Secreted effector protein SptP

Including the following 2 domains:

  1. GTPase-activating protein
    Short name=GAP
  2. Tyrosine-protein phosphatase
    EC=3.1.3.48
Gene names
Name:sptP
Ordered Locus Names:SPA2736
OrganismSalmonella paratyphi A (strain ATCC 9150 / SARB42) [Complete proteome] [HAMAP]
Taxonomic identifier295319 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella

Protein attributes

Sequence length543 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Effector proteins function to alter host cell physiology and promote bacterial survival in host tissues. This protein includes tyrosine phosphatase and GTPase activating protein (GAP) activities. After bacterial internalization, GAP mediates the reversal of the cytoskeletal changes induced by SopE. This function is independent of its tyrosine phosphatase activity, which remains unclear By similarity.

Catalytic activity

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Subunit structure

Forms a complex with SicP By similarity.

Subcellular location

Secreted By similarity. Host cytoplasm By similarity. Note: Secreted via type III secretion system 1 (SPI-1 TTSS), and delivered into the host cytoplasm By similarity.

Miscellaneous

Requires SicP as a chaperone for its stability and secretion By similarity.

Sequence similarities

In the N-terminal section; belongs to the YopE family.

Contains 1 tyrosine-protein phosphatase domain.

Ontologies

Keywords
   Biological processVirulence
   Cellular componentHost cytoplasm
Secreted
   Molecular functionGTPase activation
Hydrolase
Protein phosphatase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processpathogenesis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular space

Inferred from electronic annotation. Source: InterPro

host cell cytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionGTPase activator activity

Inferred from electronic annotation. Source: UniProtKB-KW

protein tyrosine phosphatase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 543543Secreted effector protein SptP
PRO_0000094864

Regions

Domain280 – 543264Tyrosine-protein phosphatase
Region35 – 139105Chaperone-binding By similarity
Region167 – 290124GAP By similarity

Sites

Active site4811Phosphocysteine intermediate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5PEA9 [UniParc].

Last modified January 4, 2005. Version 1.
Checksum: BE20AFE173E8C1D3

FASTA54360,005
        10         20         30         40         50         60 
MLRYEERKLN NLTLSSFSKS GVSSDTRLYI AKENTDKAYV APEKFSSKVL TWLGKMPLFK 

        70         80         90        100        110        120 
NTEVVQKHTE NIRVQNQKIL QTFLQALTEK YGEKAVNNAL YMSSINMNKP LTQRLVVQIT 

       130        140        150        160        170        180 
ECVKGADGGF INIIKNKDNV GVMNAALVIK GGDTKVTEQN NDVGAESKQP LLDIALKGLK 

       190        200        210        220        230        240 
RTIPQLEQMD GNSLRENFQE MASGNGPLRS LMTNLQSLNK IPEAKQLNDY VTTLKNIQIG 

       250        260        270        280        290        300 
ADRFSQWGTC GGEVERWIDK ASTHELTQAV KKIHVIAKEL KNVTAEFEKI KAGASMPQTM 

       310        320        330        340        350        360 
NGPTLGLARF AVSSIPINQQ TQVKLSDGMP VPVNTLTFDG KPVALAGSYP KNTPDALEAH 

       370        380        390        400        410        420 
MKMLLEKECS CLAVLTSEDQ MQAKQLPAYF RGSYTFGEVH TNSQKVSSAS QGGAIDQYNM 

       430        440        450        460        470        480 
QLSCGEKRYT IPVLHVKNWP DHQPLPSTDQ LEYLADRVKN SNQNGAPGRS SSDKHLPMIH 

       490        500        510        520        530        540 
CLGGVGRTGT MAAALVLKDN PHSNLEQVRA DFRNSRNNRM LEDASQFVQL KAMQAQLLMT 


TAS 

« Hide

References

[1]"Comparison of genome degradation in Paratyphi A and Typhi, human-restricted serovars of Salmonella enterica that cause typhoid."
McClelland M., Sanderson K.E., Clifton S.W., Latreille P., Porwollik S., Sabo A., Meyer R., Bieri T., Ozersky P., McLellan M., Harkins C.R., Wang C., Nguyen C., Berghoff A., Elliott G., Kohlberg S., Strong C., Du F. expand/collapse author list , Carter J., Kremizki C., Layman D., Leonard S., Sun H., Fulton L., Nash W., Miner T., Minx P., Delehaunty K., Fronick C., Magrini V., Nhan M., Warren W., Florea L., Spieth J., Wilson R.K.
Nat. Genet. 36:1268-1274(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 9150 / SARB42.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000026 Genomic DNA. Translation: AAV78593.1.
RefSeqYP_151905.1. NC_006511.1.

3D structure databases

ProteinModelPortalQ5PEA9.
SMRQ5PEA9. Positions 36-139, 167-539.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING295319.SPA2736.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAV78593; AAV78593; SPA2736.
PATRIC32355024. VBISalEnt134188_2910.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGNOG74835.
HOGENOMHOG000028733.
OMAWIDKAST.
OrthoDBEOG6423BT.

Enzyme and pathway databases

BioCycSENT295319:GJBZ-2734-MONOMER.

Family and domain databases

Gene3D1.20.120.260. 1 hit.
3.90.190.10. 2 hits.
InterProIPR011070. Globular_prot_asu/bsu.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR015203. SicP-binding.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
IPR003546. Tyr_Pase_SptP/YopH.
IPR014773. Uncharacterised_dom_YopE.
[Graphical view]
PfamPF09119. SicP-binding. 1 hit.
PF00102. Y_phosphatase. 1 hit.
PF03545. YopE. 1 hit.
[Graphical view]
PRINTSPR01371. BACYPHPHTASE.
SMARTSM00194. PTPc. 1 hit.
[Graphical view]
SUPFAMSSF47233. SSF47233. 1 hit.
SSF52799. SSF52799. 1 hit.
SSF56568. SSF56568. 1 hit.
PROSITEPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSPTP_SALPA
AccessionPrimary (citable) accession number: Q5PEA9
Entry history
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: January 4, 2005
Last modified: June 11, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families